Cargando…
Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Influences Artemisia annua Plant Parameters and Artemisinin Content under Different Soil Types and Cultivation Methods
Artemisinin extracted from Artemisia annua has been used efficiently in malaria treatment since 2005. In this study, the variations in plant parameters (plant biomass, glandular trichome density, essential oil total chemical content, artemisinin production, and polyphenol oxidase (PPO) activity) wer...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356791/ https://www.ncbi.nlm.nih.gov/pubmed/32549234 http://dx.doi.org/10.3390/microorganisms8060899 |
_version_ | 1783558563937910784 |
---|---|
author | Domokos, Erzsébet Bíró-Janka, Béla Bálint, János Molnár, Katalin Fazakas, Csaba Jakab-Farkas, László Domokos, József Albert, Csilla Mara, Gyöngyvér Balog, Adalbert |
author_facet | Domokos, Erzsébet Bíró-Janka, Béla Bálint, János Molnár, Katalin Fazakas, Csaba Jakab-Farkas, László Domokos, József Albert, Csilla Mara, Gyöngyvér Balog, Adalbert |
author_sort | Domokos, Erzsébet |
collection | PubMed |
description | Artemisinin extracted from Artemisia annua has been used efficiently in malaria treatment since 2005. In this study, the variations in plant parameters (plant biomass, glandular trichome density, essential oil total chemical content, artemisinin production, and polyphenol oxidase (PPO) activity) were tested under different soil types (Luvisol, Gleysol, Anthrosol and sterile peat) and cultivation conditions (potted plants in semi-open field, and open field experiments) for plants inoculated with arbuscular mycorrhizal fungus (AMF) Rizophagus irregularis. Under semi-open field conditions, the AMF colonization of A. annua plant roots varied, and presented the highest percentage in Luvisol and sterile peat. The increase in the root colonization rate positively influenced some plant parameters (biomass, glandular trichome density, artemisinin concentration, essential oil quantity and composition), but no effects on PPO enzyme activity were detected. AMF fungus R. irregularis significantly increased the artemisinin content and essential oil yield of plants cultivated in Luvisol, Gleysol, Anthrosol and in peat. These soil types can offer appropriate conditions for A. annua cultivation and artemisinin production even on a smaller scale. Under open field conditions, low (about 5%) AMF colonization was observed. No differences in artemisin contents were detected, but essential oil yield significantly increased compared to control plants. AMF treatment increased beta-farnesene and germacrene D concentrations in Artemisia plants in the open field experiment. |
format | Online Article Text |
id | pubmed-7356791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73567912020-07-22 Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Influences Artemisia annua Plant Parameters and Artemisinin Content under Different Soil Types and Cultivation Methods Domokos, Erzsébet Bíró-Janka, Béla Bálint, János Molnár, Katalin Fazakas, Csaba Jakab-Farkas, László Domokos, József Albert, Csilla Mara, Gyöngyvér Balog, Adalbert Microorganisms Article Artemisinin extracted from Artemisia annua has been used efficiently in malaria treatment since 2005. In this study, the variations in plant parameters (plant biomass, glandular trichome density, essential oil total chemical content, artemisinin production, and polyphenol oxidase (PPO) activity) were tested under different soil types (Luvisol, Gleysol, Anthrosol and sterile peat) and cultivation conditions (potted plants in semi-open field, and open field experiments) for plants inoculated with arbuscular mycorrhizal fungus (AMF) Rizophagus irregularis. Under semi-open field conditions, the AMF colonization of A. annua plant roots varied, and presented the highest percentage in Luvisol and sterile peat. The increase in the root colonization rate positively influenced some plant parameters (biomass, glandular trichome density, artemisinin concentration, essential oil quantity and composition), but no effects on PPO enzyme activity were detected. AMF fungus R. irregularis significantly increased the artemisinin content and essential oil yield of plants cultivated in Luvisol, Gleysol, Anthrosol and in peat. These soil types can offer appropriate conditions for A. annua cultivation and artemisinin production even on a smaller scale. Under open field conditions, low (about 5%) AMF colonization was observed. No differences in artemisin contents were detected, but essential oil yield significantly increased compared to control plants. AMF treatment increased beta-farnesene and germacrene D concentrations in Artemisia plants in the open field experiment. MDPI 2020-06-15 /pmc/articles/PMC7356791/ /pubmed/32549234 http://dx.doi.org/10.3390/microorganisms8060899 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Domokos, Erzsébet Bíró-Janka, Béla Bálint, János Molnár, Katalin Fazakas, Csaba Jakab-Farkas, László Domokos, József Albert, Csilla Mara, Gyöngyvér Balog, Adalbert Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Influences Artemisia annua Plant Parameters and Artemisinin Content under Different Soil Types and Cultivation Methods |
title | Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Influences Artemisia annua Plant Parameters and Artemisinin Content under Different Soil Types and Cultivation Methods |
title_full | Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Influences Artemisia annua Plant Parameters and Artemisinin Content under Different Soil Types and Cultivation Methods |
title_fullStr | Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Influences Artemisia annua Plant Parameters and Artemisinin Content under Different Soil Types and Cultivation Methods |
title_full_unstemmed | Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Influences Artemisia annua Plant Parameters and Artemisinin Content under Different Soil Types and Cultivation Methods |
title_short | Arbuscular Mycorrhizal Fungus Rhizophagus irregularis Influences Artemisia annua Plant Parameters and Artemisinin Content under Different Soil Types and Cultivation Methods |
title_sort | arbuscular mycorrhizal fungus rhizophagus irregularis influences artemisia annua plant parameters and artemisinin content under different soil types and cultivation methods |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356791/ https://www.ncbi.nlm.nih.gov/pubmed/32549234 http://dx.doi.org/10.3390/microorganisms8060899 |
work_keys_str_mv | AT domokoserzsebet arbuscularmycorrhizalfungusrhizophagusirregularisinfluencesartemisiaannuaplantparametersandartemisinincontentunderdifferentsoiltypesandcultivationmethods AT birojankabela arbuscularmycorrhizalfungusrhizophagusirregularisinfluencesartemisiaannuaplantparametersandartemisinincontentunderdifferentsoiltypesandcultivationmethods AT balintjanos arbuscularmycorrhizalfungusrhizophagusirregularisinfluencesartemisiaannuaplantparametersandartemisinincontentunderdifferentsoiltypesandcultivationmethods AT molnarkatalin arbuscularmycorrhizalfungusrhizophagusirregularisinfluencesartemisiaannuaplantparametersandartemisinincontentunderdifferentsoiltypesandcultivationmethods AT fazakascsaba arbuscularmycorrhizalfungusrhizophagusirregularisinfluencesartemisiaannuaplantparametersandartemisinincontentunderdifferentsoiltypesandcultivationmethods AT jakabfarkaslaszlo arbuscularmycorrhizalfungusrhizophagusirregularisinfluencesartemisiaannuaplantparametersandartemisinincontentunderdifferentsoiltypesandcultivationmethods AT domokosjozsef arbuscularmycorrhizalfungusrhizophagusirregularisinfluencesartemisiaannuaplantparametersandartemisinincontentunderdifferentsoiltypesandcultivationmethods AT albertcsilla arbuscularmycorrhizalfungusrhizophagusirregularisinfluencesartemisiaannuaplantparametersandartemisinincontentunderdifferentsoiltypesandcultivationmethods AT maragyongyver arbuscularmycorrhizalfungusrhizophagusirregularisinfluencesartemisiaannuaplantparametersandartemisinincontentunderdifferentsoiltypesandcultivationmethods AT balogadalbert arbuscularmycorrhizalfungusrhizophagusirregularisinfluencesartemisiaannuaplantparametersandartemisinincontentunderdifferentsoiltypesandcultivationmethods |