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Inhibiting Methanogenesis Stimulated de novo Synthesis of Microbial Amino Acids in Mixed Rumen Batch Cultures Growing on Starch but not on Cellulose
Ameliorating methane (CH(4)) emissions from ruminants would have environmental benefits, but it is necessary to redirect metabolic hydrogen ([H]) toward useful sinks to also benefit animal productivity. We hypothesized that inhibiting rumen methanogenesis would increase de novo synthesis of microbia...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356843/ https://www.ncbi.nlm.nih.gov/pubmed/32466548 http://dx.doi.org/10.3390/microorganisms8060799 |
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author | Ungerfeld, Emilio M. Aedo, M. Fernanda Muñoz, Camila Urrutia, Natalie L. Martínez, Emilio D. Saldivia, Marcelo |
author_facet | Ungerfeld, Emilio M. Aedo, M. Fernanda Muñoz, Camila Urrutia, Natalie L. Martínez, Emilio D. Saldivia, Marcelo |
author_sort | Ungerfeld, Emilio M. |
collection | PubMed |
description | Ameliorating methane (CH(4)) emissions from ruminants would have environmental benefits, but it is necessary to redirect metabolic hydrogen ([H]) toward useful sinks to also benefit animal productivity. We hypothesized that inhibiting rumen methanogenesis would increase de novo synthesis of microbial amino acids (AA) as an alternative [H] sink if sufficient energy and carbon are provided. We examined the effects of inhibiting methanogenesis with 9, 10-anthraquione (AQ) on mixed rumen batch cultures growing on cellulose or starch as sources of energy and carbon contrasting in fermentability, with ammonium (NH(4)(+)) or trypticase (Try) as nitrogen (N) sources. Inhibiting methanogenesis with AQ inhibited digestion with cellulose but not with starch, and decreased propionate and increased butyrate molar percentages with both substrates. Inhibiting methanogenesis with 9, 10-anthraquinone increased de novo synthesis of microbial AA with starch but not with cellulose. The decrease in the recovery of [H] caused by the inhibition of methanogenesis was more moderate with starch due to an enhancement of butyrate and AA as [H] sinks. There may be an opportunity to simultaneously decrease the emissions of CH(4) and N with some ruminant diets and replace plant protein supplements with less expensive non-protein nitrogen sources such as urea. |
format | Online Article Text |
id | pubmed-7356843 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73568432020-07-22 Inhibiting Methanogenesis Stimulated de novo Synthesis of Microbial Amino Acids in Mixed Rumen Batch Cultures Growing on Starch but not on Cellulose Ungerfeld, Emilio M. Aedo, M. Fernanda Muñoz, Camila Urrutia, Natalie L. Martínez, Emilio D. Saldivia, Marcelo Microorganisms Article Ameliorating methane (CH(4)) emissions from ruminants would have environmental benefits, but it is necessary to redirect metabolic hydrogen ([H]) toward useful sinks to also benefit animal productivity. We hypothesized that inhibiting rumen methanogenesis would increase de novo synthesis of microbial amino acids (AA) as an alternative [H] sink if sufficient energy and carbon are provided. We examined the effects of inhibiting methanogenesis with 9, 10-anthraquione (AQ) on mixed rumen batch cultures growing on cellulose or starch as sources of energy and carbon contrasting in fermentability, with ammonium (NH(4)(+)) or trypticase (Try) as nitrogen (N) sources. Inhibiting methanogenesis with AQ inhibited digestion with cellulose but not with starch, and decreased propionate and increased butyrate molar percentages with both substrates. Inhibiting methanogenesis with 9, 10-anthraquinone increased de novo synthesis of microbial AA with starch but not with cellulose. The decrease in the recovery of [H] caused by the inhibition of methanogenesis was more moderate with starch due to an enhancement of butyrate and AA as [H] sinks. There may be an opportunity to simultaneously decrease the emissions of CH(4) and N with some ruminant diets and replace plant protein supplements with less expensive non-protein nitrogen sources such as urea. MDPI 2020-05-26 /pmc/articles/PMC7356843/ /pubmed/32466548 http://dx.doi.org/10.3390/microorganisms8060799 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ungerfeld, Emilio M. Aedo, M. Fernanda Muñoz, Camila Urrutia, Natalie L. Martínez, Emilio D. Saldivia, Marcelo Inhibiting Methanogenesis Stimulated de novo Synthesis of Microbial Amino Acids in Mixed Rumen Batch Cultures Growing on Starch but not on Cellulose |
title | Inhibiting Methanogenesis Stimulated de novo Synthesis of Microbial Amino Acids in Mixed Rumen Batch Cultures Growing on Starch but not on Cellulose |
title_full | Inhibiting Methanogenesis Stimulated de novo Synthesis of Microbial Amino Acids in Mixed Rumen Batch Cultures Growing on Starch but not on Cellulose |
title_fullStr | Inhibiting Methanogenesis Stimulated de novo Synthesis of Microbial Amino Acids in Mixed Rumen Batch Cultures Growing on Starch but not on Cellulose |
title_full_unstemmed | Inhibiting Methanogenesis Stimulated de novo Synthesis of Microbial Amino Acids in Mixed Rumen Batch Cultures Growing on Starch but not on Cellulose |
title_short | Inhibiting Methanogenesis Stimulated de novo Synthesis of Microbial Amino Acids in Mixed Rumen Batch Cultures Growing on Starch but not on Cellulose |
title_sort | inhibiting methanogenesis stimulated de novo synthesis of microbial amino acids in mixed rumen batch cultures growing on starch but not on cellulose |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356843/ https://www.ncbi.nlm.nih.gov/pubmed/32466548 http://dx.doi.org/10.3390/microorganisms8060799 |
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