Cargando…
Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example
Potato (Solanum tuberosum) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caus...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356882/ https://www.ncbi.nlm.nih.gov/pubmed/32486039 http://dx.doi.org/10.3390/plants9060695 |
_version_ | 1783558584674549760 |
---|---|
author | Wang, Eu Sheng Kieu, Nam Phuong Lenman, Marit Andreasson, Erik |
author_facet | Wang, Eu Sheng Kieu, Nam Phuong Lenman, Marit Andreasson, Erik |
author_sort | Wang, Eu Sheng |
collection | PubMed |
description | Potato (Solanum tuberosum) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caused by Phytophthora infestans. Stacking of resistance (R) genes from wild Solanum relatives are interesting prospects for the sustainable control of late blight. Therefore, we optimized methods for the efficient generation and screening of R-gene-containing transformants in tetraploid and diploid hybrid potato genotypes. Using these methods, a high transformation efficiency was achieved for the transformation of tetraploid and diploid potato lines with a triple resistance (3R) gene construct. Transformation efficiencies were improved by optimizing several factors affecting regeneration, including the quality of the starting plant material, and the composition of the plant growth regulators used during selective regeneration. A refreshment protocol was designed to alleviate in vitro related stress in stock plants, which significantly improved the growth vigor and resulted in a 4- to 10-fold increase in transformation efficiency. Furthermore, long-term exposure to exogenous Indole-3-butyric acid that is usually used for the initiation of roots in vitro, was found to cause aberrant morphological phenotypes in potato. |
format | Online Article Text |
id | pubmed-7356882 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73568822020-07-22 Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example Wang, Eu Sheng Kieu, Nam Phuong Lenman, Marit Andreasson, Erik Plants (Basel) Article Potato (Solanum tuberosum) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caused by Phytophthora infestans. Stacking of resistance (R) genes from wild Solanum relatives are interesting prospects for the sustainable control of late blight. Therefore, we optimized methods for the efficient generation and screening of R-gene-containing transformants in tetraploid and diploid hybrid potato genotypes. Using these methods, a high transformation efficiency was achieved for the transformation of tetraploid and diploid potato lines with a triple resistance (3R) gene construct. Transformation efficiencies were improved by optimizing several factors affecting regeneration, including the quality of the starting plant material, and the composition of the plant growth regulators used during selective regeneration. A refreshment protocol was designed to alleviate in vitro related stress in stock plants, which significantly improved the growth vigor and resulted in a 4- to 10-fold increase in transformation efficiency. Furthermore, long-term exposure to exogenous Indole-3-butyric acid that is usually used for the initiation of roots in vitro, was found to cause aberrant morphological phenotypes in potato. MDPI 2020-05-29 /pmc/articles/PMC7356882/ /pubmed/32486039 http://dx.doi.org/10.3390/plants9060695 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Eu Sheng Kieu, Nam Phuong Lenman, Marit Andreasson, Erik Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example |
title | Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example |
title_full | Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example |
title_fullStr | Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example |
title_full_unstemmed | Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example |
title_short | Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example |
title_sort | tissue culture and refreshment techniques for improvement of transformation in local tetraploid and diploid potato with late blight resistance as an example |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356882/ https://www.ncbi.nlm.nih.gov/pubmed/32486039 http://dx.doi.org/10.3390/plants9060695 |
work_keys_str_mv | AT wangeusheng tissuecultureandrefreshmenttechniquesforimprovementoftransformationinlocaltetraploidanddiploidpotatowithlateblightresistanceasanexample AT kieunamphuong tissuecultureandrefreshmenttechniquesforimprovementoftransformationinlocaltetraploidanddiploidpotatowithlateblightresistanceasanexample AT lenmanmarit tissuecultureandrefreshmenttechniquesforimprovementoftransformationinlocaltetraploidanddiploidpotatowithlateblightresistanceasanexample AT andreassonerik tissuecultureandrefreshmenttechniquesforimprovementoftransformationinlocaltetraploidanddiploidpotatowithlateblightresistanceasanexample |