Cargando…

Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example

Potato (Solanum tuberosum) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caus...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Eu Sheng, Kieu, Nam Phuong, Lenman, Marit, Andreasson, Erik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356882/
https://www.ncbi.nlm.nih.gov/pubmed/32486039
http://dx.doi.org/10.3390/plants9060695
_version_ 1783558584674549760
author Wang, Eu Sheng
Kieu, Nam Phuong
Lenman, Marit
Andreasson, Erik
author_facet Wang, Eu Sheng
Kieu, Nam Phuong
Lenman, Marit
Andreasson, Erik
author_sort Wang, Eu Sheng
collection PubMed
description Potato (Solanum tuberosum) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caused by Phytophthora infestans. Stacking of resistance (R) genes from wild Solanum relatives are interesting prospects for the sustainable control of late blight. Therefore, we optimized methods for the efficient generation and screening of R-gene-containing transformants in tetraploid and diploid hybrid potato genotypes. Using these methods, a high transformation efficiency was achieved for the transformation of tetraploid and diploid potato lines with a triple resistance (3R) gene construct. Transformation efficiencies were improved by optimizing several factors affecting regeneration, including the quality of the starting plant material, and the composition of the plant growth regulators used during selective regeneration. A refreshment protocol was designed to alleviate in vitro related stress in stock plants, which significantly improved the growth vigor and resulted in a 4- to 10-fold increase in transformation efficiency. Furthermore, long-term exposure to exogenous Indole-3-butyric acid that is usually used for the initiation of roots in vitro, was found to cause aberrant morphological phenotypes in potato.
format Online
Article
Text
id pubmed-7356882
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73568822020-07-22 Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example Wang, Eu Sheng Kieu, Nam Phuong Lenman, Marit Andreasson, Erik Plants (Basel) Article Potato (Solanum tuberosum) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caused by Phytophthora infestans. Stacking of resistance (R) genes from wild Solanum relatives are interesting prospects for the sustainable control of late blight. Therefore, we optimized methods for the efficient generation and screening of R-gene-containing transformants in tetraploid and diploid hybrid potato genotypes. Using these methods, a high transformation efficiency was achieved for the transformation of tetraploid and diploid potato lines with a triple resistance (3R) gene construct. Transformation efficiencies were improved by optimizing several factors affecting regeneration, including the quality of the starting plant material, and the composition of the plant growth regulators used during selective regeneration. A refreshment protocol was designed to alleviate in vitro related stress in stock plants, which significantly improved the growth vigor and resulted in a 4- to 10-fold increase in transformation efficiency. Furthermore, long-term exposure to exogenous Indole-3-butyric acid that is usually used for the initiation of roots in vitro, was found to cause aberrant morphological phenotypes in potato. MDPI 2020-05-29 /pmc/articles/PMC7356882/ /pubmed/32486039 http://dx.doi.org/10.3390/plants9060695 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Eu Sheng
Kieu, Nam Phuong
Lenman, Marit
Andreasson, Erik
Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example
title Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example
title_full Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example
title_fullStr Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example
title_full_unstemmed Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example
title_short Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example
title_sort tissue culture and refreshment techniques for improvement of transformation in local tetraploid and diploid potato with late blight resistance as an example
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356882/
https://www.ncbi.nlm.nih.gov/pubmed/32486039
http://dx.doi.org/10.3390/plants9060695
work_keys_str_mv AT wangeusheng tissuecultureandrefreshmenttechniquesforimprovementoftransformationinlocaltetraploidanddiploidpotatowithlateblightresistanceasanexample
AT kieunamphuong tissuecultureandrefreshmenttechniquesforimprovementoftransformationinlocaltetraploidanddiploidpotatowithlateblightresistanceasanexample
AT lenmanmarit tissuecultureandrefreshmenttechniquesforimprovementoftransformationinlocaltetraploidanddiploidpotatowithlateblightresistanceasanexample
AT andreassonerik tissuecultureandrefreshmenttechniquesforimprovementoftransformationinlocaltetraploidanddiploidpotatowithlateblightresistanceasanexample