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Clinicopathological Significance of RUNX1 in Non-Small Cell Lung Cancer
This study aimed to understand the clinicopathological significance of runt-related transcription factor 1 (RUNX1) in non-small cell lung cancer (NSCLC). The methylation and mRNA levels of RUNX1 in NSCLC were determined using the Infinium HumanMethylation450 BeadChip and the HumanHT-12 expression Be...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356912/ https://www.ncbi.nlm.nih.gov/pubmed/32498288 http://dx.doi.org/10.3390/jcm9061694 |
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author | Kim, Yujin Lee, Bo Bin Kim, Dongho Um, Sangwon Cho, Eun Yoon Han, Joungho Shim, Young Mog Kim, Duk-Hwan |
author_facet | Kim, Yujin Lee, Bo Bin Kim, Dongho Um, Sangwon Cho, Eun Yoon Han, Joungho Shim, Young Mog Kim, Duk-Hwan |
author_sort | Kim, Yujin |
collection | PubMed |
description | This study aimed to understand the clinicopathological significance of runt-related transcription factor 1 (RUNX1) in non-small cell lung cancer (NSCLC). The methylation and mRNA levels of RUNX1 in NSCLC were determined using the Infinium HumanMethylation450 BeadChip and the HumanHT-12 expression BeadChip. RUNX1 protein levels were analyzed using immunohistochemistry of formalin-fixed paraffin-embedded tissues from 409 NSCLC patients. Three CpGs (cg04228935, cg11498607, and cg05000748) in the CpG island of RUNX1 showed significantly different methylation levels (Bonferroni corrected p < 0.05) between tumor and matched normal tissues obtained from 42 NSCLC patients. Methylation levels of the CpGs in the tumor tissues were inversely related to mRNA levels of RUNX1. A logistic regression model based on cg04228935 showed the best performance in predicting NSCLCs in a test dataset (N = 28) with the area under the receiver operating characteristic (ROC) curve (AUC) of 0.96 (95% confidence interval (CI) = 0.81–0.99). The expression of RUNX1 was reduced in 125 (31%) of 409 patients. Adenocarcinoma patients with reduced RUNX1 expression showed 1.97-fold (95% confidence interval = 1.16–3.44, p = 0.01) higher hazard ratio for death than those without. In conclusion, the present study suggests that abnormal methylation of RUNX1 may be a valuable biomarker for detection of NSCLC regardless of race. And, reduced RUNX1 expression may be a prognostic indicator of poor overall survival in lung adenocarcinoma. |
format | Online Article Text |
id | pubmed-7356912 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73569122020-07-22 Clinicopathological Significance of RUNX1 in Non-Small Cell Lung Cancer Kim, Yujin Lee, Bo Bin Kim, Dongho Um, Sangwon Cho, Eun Yoon Han, Joungho Shim, Young Mog Kim, Duk-Hwan J Clin Med Article This study aimed to understand the clinicopathological significance of runt-related transcription factor 1 (RUNX1) in non-small cell lung cancer (NSCLC). The methylation and mRNA levels of RUNX1 in NSCLC were determined using the Infinium HumanMethylation450 BeadChip and the HumanHT-12 expression BeadChip. RUNX1 protein levels were analyzed using immunohistochemistry of formalin-fixed paraffin-embedded tissues from 409 NSCLC patients. Three CpGs (cg04228935, cg11498607, and cg05000748) in the CpG island of RUNX1 showed significantly different methylation levels (Bonferroni corrected p < 0.05) between tumor and matched normal tissues obtained from 42 NSCLC patients. Methylation levels of the CpGs in the tumor tissues were inversely related to mRNA levels of RUNX1. A logistic regression model based on cg04228935 showed the best performance in predicting NSCLCs in a test dataset (N = 28) with the area under the receiver operating characteristic (ROC) curve (AUC) of 0.96 (95% confidence interval (CI) = 0.81–0.99). The expression of RUNX1 was reduced in 125 (31%) of 409 patients. Adenocarcinoma patients with reduced RUNX1 expression showed 1.97-fold (95% confidence interval = 1.16–3.44, p = 0.01) higher hazard ratio for death than those without. In conclusion, the present study suggests that abnormal methylation of RUNX1 may be a valuable biomarker for detection of NSCLC regardless of race. And, reduced RUNX1 expression may be a prognostic indicator of poor overall survival in lung adenocarcinoma. MDPI 2020-06-02 /pmc/articles/PMC7356912/ /pubmed/32498288 http://dx.doi.org/10.3390/jcm9061694 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Yujin Lee, Bo Bin Kim, Dongho Um, Sangwon Cho, Eun Yoon Han, Joungho Shim, Young Mog Kim, Duk-Hwan Clinicopathological Significance of RUNX1 in Non-Small Cell Lung Cancer |
title | Clinicopathological Significance of RUNX1 in Non-Small Cell Lung Cancer |
title_full | Clinicopathological Significance of RUNX1 in Non-Small Cell Lung Cancer |
title_fullStr | Clinicopathological Significance of RUNX1 in Non-Small Cell Lung Cancer |
title_full_unstemmed | Clinicopathological Significance of RUNX1 in Non-Small Cell Lung Cancer |
title_short | Clinicopathological Significance of RUNX1 in Non-Small Cell Lung Cancer |
title_sort | clinicopathological significance of runx1 in non-small cell lung cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356912/ https://www.ncbi.nlm.nih.gov/pubmed/32498288 http://dx.doi.org/10.3390/jcm9061694 |
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