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Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding

One of the limitations in obtaining the genetic diversity of doubled haploid (DH) lines via anther culture is the development of families of regenerants, and each family represents a clone. This work examines the results of studying this phenomenon in anther culture of alloplasmic (H. vulgare)–T. ae...

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Autores principales: Pershina, Lidiya, Trubacheeva, Nataliya, Badaeva, Ekaterina, Belan, Igor, Rosseeva, Ludmila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356915/
https://www.ncbi.nlm.nih.gov/pubmed/32570980
http://dx.doi.org/10.3390/plants9060764
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author Pershina, Lidiya
Trubacheeva, Nataliya
Badaeva, Ekaterina
Belan, Igor
Rosseeva, Ludmila
author_facet Pershina, Lidiya
Trubacheeva, Nataliya
Badaeva, Ekaterina
Belan, Igor
Rosseeva, Ludmila
author_sort Pershina, Lidiya
collection PubMed
description One of the limitations in obtaining the genetic diversity of doubled haploid (DH) lines via anther culture is the development of families of regenerants, and each family represents a clone. This work examines the results of studying this phenomenon in anther culture of alloplasmic (H. vulgare)–T. aestivum and euplasmic lines with 1RS.1BL and 7DL-7Ai translocations and hybrids between them. Parameters of androgenesis such as the number of embryo-like structures, the total number of regenerants, and the number of green regenerants per 100 anthers varied depending on the genotype. In all genotypes from embryo-like structures, predominant development of families of plantlets rather than single plantlets was found. The source of family plantlets was polyembryos. About 75% of families consisted of regenerants at the same fertility level. On average, 37.74%4% of the R0 plants were fertile. The sister DH lines of three hybrid combinations were formed from seeds of R1 plants (2n = 42) with high fertility and in the presence of wheat–alien translocations. After four years of breeding trials, the sister DH lines of three families with fungal disease resistance increased yield, and some parameters of grain quality exceeding the controls were identified as promising for breeding.
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spelling pubmed-73569152020-07-22 Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding Pershina, Lidiya Trubacheeva, Nataliya Badaeva, Ekaterina Belan, Igor Rosseeva, Ludmila Plants (Basel) Article One of the limitations in obtaining the genetic diversity of doubled haploid (DH) lines via anther culture is the development of families of regenerants, and each family represents a clone. This work examines the results of studying this phenomenon in anther culture of alloplasmic (H. vulgare)–T. aestivum and euplasmic lines with 1RS.1BL and 7DL-7Ai translocations and hybrids between them. Parameters of androgenesis such as the number of embryo-like structures, the total number of regenerants, and the number of green regenerants per 100 anthers varied depending on the genotype. In all genotypes from embryo-like structures, predominant development of families of plantlets rather than single plantlets was found. The source of family plantlets was polyembryos. About 75% of families consisted of regenerants at the same fertility level. On average, 37.74%4% of the R0 plants were fertile. The sister DH lines of three hybrid combinations were formed from seeds of R1 plants (2n = 42) with high fertility and in the presence of wheat–alien translocations. After four years of breeding trials, the sister DH lines of three families with fungal disease resistance increased yield, and some parameters of grain quality exceeding the controls were identified as promising for breeding. MDPI 2020-06-18 /pmc/articles/PMC7356915/ /pubmed/32570980 http://dx.doi.org/10.3390/plants9060764 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pershina, Lidiya
Trubacheeva, Nataliya
Badaeva, Ekaterina
Belan, Igor
Rosseeva, Ludmila
Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding
title Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding
title_full Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding
title_fullStr Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding
title_full_unstemmed Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding
title_short Study of Androgenic Plant Families of Alloplasmic Introgression Lines (H. vulgare) –T. aestivum and the Use of Sister DH Lines in Breeding
title_sort study of androgenic plant families of alloplasmic introgression lines (h. vulgare) –t. aestivum and the use of sister dh lines in breeding
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7356915/
https://www.ncbi.nlm.nih.gov/pubmed/32570980
http://dx.doi.org/10.3390/plants9060764
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