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Differentially Abundant Bacterial Taxa Associated with Prognostic Variables of Crohn’s Disease: Results from the IMPACT Study
Limited studies have examined the intestinal microbiota composition in relation to Crohn’s disease (CD) prognosis. We analyzed the differences in microbial communities and relevant metabolic pathways associated with prognostic variables in patients with CD. We applied 16S rRNA gene sequencing to ana...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357029/ https://www.ncbi.nlm.nih.gov/pubmed/32516912 http://dx.doi.org/10.3390/jcm9061748 |
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author | Park, Soo-kyung Kim, Han-Na Choi, Chang Hwan Im, Jong Pil Cha, Jae Myung Eun, Chang Soo Kim, Tae-Oh Kang, Sang-Bum Bang, Ki Bae Kim, Hyun Gun Jung, Yunho Yoon, Hyuk Han, Dong-Soo Lee, Chil-Woo Ahn, Kwangsung Kim, Hyung-Lae Park, Dong Il |
author_facet | Park, Soo-kyung Kim, Han-Na Choi, Chang Hwan Im, Jong Pil Cha, Jae Myung Eun, Chang Soo Kim, Tae-Oh Kang, Sang-Bum Bang, Ki Bae Kim, Hyun Gun Jung, Yunho Yoon, Hyuk Han, Dong-Soo Lee, Chil-Woo Ahn, Kwangsung Kim, Hyung-Lae Park, Dong Il |
author_sort | Park, Soo-kyung |
collection | PubMed |
description | Limited studies have examined the intestinal microbiota composition in relation to Crohn’s disease (CD) prognosis. We analyzed the differences in microbial communities and relevant metabolic pathways associated with prognostic variables in patients with CD. We applied 16S rRNA gene sequencing to analyze a cohort of 1110 CD and healthy control (HC) fecal samples. We categorized patients with CD into good (CD-G), intermediate (CD-I) and poor (CD-P) prognosis groups, according to the history of using biologics and intestinal resection. Microbiota α-diversity decreased more in CD-P than CD-G and CD-I. Microbiota ß-diversity in CD-P differed from that in CD-G and CD-I. Thirteen genera and 10 species showed differential abundance between CD-G and CD-P groups. Escherichia coli (p = 0.001) and species Producta (p = 0.01) and genera Lactobacillus (p = 0.003) and Coprococcus (p = 0.01) consistently showed differences between CD-G and CD-P groups after adjusting for confounding variables. Functional profiling suggested that the microbial catabolic pathways and pathways related to enterobacterial common antigen and lipopolysaccharide biosynthesis were better represented in the CD-P group than in the CD-G group, and E. coli were the top contributors to these pathways. CD prognosis is associated with altered microbiota composition and decreased diversity, and E. coli might be causally involved in CD progression, and may have adapted to live in inflammatory environments. |
format | Online Article Text |
id | pubmed-7357029 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73570292020-07-23 Differentially Abundant Bacterial Taxa Associated with Prognostic Variables of Crohn’s Disease: Results from the IMPACT Study Park, Soo-kyung Kim, Han-Na Choi, Chang Hwan Im, Jong Pil Cha, Jae Myung Eun, Chang Soo Kim, Tae-Oh Kang, Sang-Bum Bang, Ki Bae Kim, Hyun Gun Jung, Yunho Yoon, Hyuk Han, Dong-Soo Lee, Chil-Woo Ahn, Kwangsung Kim, Hyung-Lae Park, Dong Il J Clin Med Article Limited studies have examined the intestinal microbiota composition in relation to Crohn’s disease (CD) prognosis. We analyzed the differences in microbial communities and relevant metabolic pathways associated with prognostic variables in patients with CD. We applied 16S rRNA gene sequencing to analyze a cohort of 1110 CD and healthy control (HC) fecal samples. We categorized patients with CD into good (CD-G), intermediate (CD-I) and poor (CD-P) prognosis groups, according to the history of using biologics and intestinal resection. Microbiota α-diversity decreased more in CD-P than CD-G and CD-I. Microbiota ß-diversity in CD-P differed from that in CD-G and CD-I. Thirteen genera and 10 species showed differential abundance between CD-G and CD-P groups. Escherichia coli (p = 0.001) and species Producta (p = 0.01) and genera Lactobacillus (p = 0.003) and Coprococcus (p = 0.01) consistently showed differences between CD-G and CD-P groups after adjusting for confounding variables. Functional profiling suggested that the microbial catabolic pathways and pathways related to enterobacterial common antigen and lipopolysaccharide biosynthesis were better represented in the CD-P group than in the CD-G group, and E. coli were the top contributors to these pathways. CD prognosis is associated with altered microbiota composition and decreased diversity, and E. coli might be causally involved in CD progression, and may have adapted to live in inflammatory environments. MDPI 2020-06-05 /pmc/articles/PMC7357029/ /pubmed/32516912 http://dx.doi.org/10.3390/jcm9061748 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Park, Soo-kyung Kim, Han-Na Choi, Chang Hwan Im, Jong Pil Cha, Jae Myung Eun, Chang Soo Kim, Tae-Oh Kang, Sang-Bum Bang, Ki Bae Kim, Hyun Gun Jung, Yunho Yoon, Hyuk Han, Dong-Soo Lee, Chil-Woo Ahn, Kwangsung Kim, Hyung-Lae Park, Dong Il Differentially Abundant Bacterial Taxa Associated with Prognostic Variables of Crohn’s Disease: Results from the IMPACT Study |
title | Differentially Abundant Bacterial Taxa Associated with Prognostic Variables of Crohn’s Disease: Results from the IMPACT Study |
title_full | Differentially Abundant Bacterial Taxa Associated with Prognostic Variables of Crohn’s Disease: Results from the IMPACT Study |
title_fullStr | Differentially Abundant Bacterial Taxa Associated with Prognostic Variables of Crohn’s Disease: Results from the IMPACT Study |
title_full_unstemmed | Differentially Abundant Bacterial Taxa Associated with Prognostic Variables of Crohn’s Disease: Results from the IMPACT Study |
title_short | Differentially Abundant Bacterial Taxa Associated with Prognostic Variables of Crohn’s Disease: Results from the IMPACT Study |
title_sort | differentially abundant bacterial taxa associated with prognostic variables of crohn’s disease: results from the impact study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357029/ https://www.ncbi.nlm.nih.gov/pubmed/32516912 http://dx.doi.org/10.3390/jcm9061748 |
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