Cargando…

Untargeted Characterization of Chestnut (Castanea sativa Mill.) Shell Polyphenol Extract: A Valued Bioresource for Prostate Cancer Cell Growth Inhibition

Chestnut seeds are used for fresh consumption and for the industrial preparation of derivatives, such as chestnut flour. During industrial processing, large amounts of by-products are generally produced, such as leaves, flowers, shells and burs. In the present study, chestnut shells were extracted b...

Descripción completa

Detalles Bibliográficos
Autores principales: Cacciola, Nunzio Antonio, Cerrato, Andrea, Capriotti, Anna Laura, Cavaliere, Chiara, D’Apolito, Maria, Montone, Carmela Maria, Piovesana, Susy, Squillaci, Giuseppe, Peluso, Gianfranco, Laganà, Aldo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357160/
https://www.ncbi.nlm.nih.gov/pubmed/32545546
http://dx.doi.org/10.3390/molecules25122730
_version_ 1783558647469572096
author Cacciola, Nunzio Antonio
Cerrato, Andrea
Capriotti, Anna Laura
Cavaliere, Chiara
D’Apolito, Maria
Montone, Carmela Maria
Piovesana, Susy
Squillaci, Giuseppe
Peluso, Gianfranco
Laganà, Aldo
author_facet Cacciola, Nunzio Antonio
Cerrato, Andrea
Capriotti, Anna Laura
Cavaliere, Chiara
D’Apolito, Maria
Montone, Carmela Maria
Piovesana, Susy
Squillaci, Giuseppe
Peluso, Gianfranco
Laganà, Aldo
author_sort Cacciola, Nunzio Antonio
collection PubMed
description Chestnut seeds are used for fresh consumption and for the industrial preparation of derivatives, such as chestnut flour. During industrial processing, large amounts of by-products are generally produced, such as leaves, flowers, shells and burs. In the present study, chestnut shells were extracted by boiling water in order to obtain polyphenol-rich extracts. Moreover, for the removal or non-phenolic compounds, a separation by preparative reverse phase chromatography in ten fractions was carried out. The richest fractions in terms of phenolic content were characterized by means of untargeted high-resolution mass spectrometric analysis together with a dedicated and customized data processing workflow. A total of 243 flavonoids, phenolic acids, proanthocyanidins and ellagitannins were tentatively identified in the five richest fractions. Due its high phenolic content (450.03 µg GAE per mg of fraction), one tumor cell line (DU 145) and one normal prostate epithelial cell line (PNT2) were exposed to increasing concentration of fraction 3 dry extract for 24, 48 and 72 h. Moreover, for DU 145 cell lines, increase of apoptotic cells and perturbation of cell cycle was demonstrated for the same extract. Those outcomes suggest that chestnut industrial by-products could be potentially employed as a source of bioresources.
format Online
Article
Text
id pubmed-7357160
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73571602020-07-23 Untargeted Characterization of Chestnut (Castanea sativa Mill.) Shell Polyphenol Extract: A Valued Bioresource for Prostate Cancer Cell Growth Inhibition Cacciola, Nunzio Antonio Cerrato, Andrea Capriotti, Anna Laura Cavaliere, Chiara D’Apolito, Maria Montone, Carmela Maria Piovesana, Susy Squillaci, Giuseppe Peluso, Gianfranco Laganà, Aldo Molecules Article Chestnut seeds are used for fresh consumption and for the industrial preparation of derivatives, such as chestnut flour. During industrial processing, large amounts of by-products are generally produced, such as leaves, flowers, shells and burs. In the present study, chestnut shells were extracted by boiling water in order to obtain polyphenol-rich extracts. Moreover, for the removal or non-phenolic compounds, a separation by preparative reverse phase chromatography in ten fractions was carried out. The richest fractions in terms of phenolic content were characterized by means of untargeted high-resolution mass spectrometric analysis together with a dedicated and customized data processing workflow. A total of 243 flavonoids, phenolic acids, proanthocyanidins and ellagitannins were tentatively identified in the five richest fractions. Due its high phenolic content (450.03 µg GAE per mg of fraction), one tumor cell line (DU 145) and one normal prostate epithelial cell line (PNT2) were exposed to increasing concentration of fraction 3 dry extract for 24, 48 and 72 h. Moreover, for DU 145 cell lines, increase of apoptotic cells and perturbation of cell cycle was demonstrated for the same extract. Those outcomes suggest that chestnut industrial by-products could be potentially employed as a source of bioresources. MDPI 2020-06-12 /pmc/articles/PMC7357160/ /pubmed/32545546 http://dx.doi.org/10.3390/molecules25122730 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cacciola, Nunzio Antonio
Cerrato, Andrea
Capriotti, Anna Laura
Cavaliere, Chiara
D’Apolito, Maria
Montone, Carmela Maria
Piovesana, Susy
Squillaci, Giuseppe
Peluso, Gianfranco
Laganà, Aldo
Untargeted Characterization of Chestnut (Castanea sativa Mill.) Shell Polyphenol Extract: A Valued Bioresource for Prostate Cancer Cell Growth Inhibition
title Untargeted Characterization of Chestnut (Castanea sativa Mill.) Shell Polyphenol Extract: A Valued Bioresource for Prostate Cancer Cell Growth Inhibition
title_full Untargeted Characterization of Chestnut (Castanea sativa Mill.) Shell Polyphenol Extract: A Valued Bioresource for Prostate Cancer Cell Growth Inhibition
title_fullStr Untargeted Characterization of Chestnut (Castanea sativa Mill.) Shell Polyphenol Extract: A Valued Bioresource for Prostate Cancer Cell Growth Inhibition
title_full_unstemmed Untargeted Characterization of Chestnut (Castanea sativa Mill.) Shell Polyphenol Extract: A Valued Bioresource for Prostate Cancer Cell Growth Inhibition
title_short Untargeted Characterization of Chestnut (Castanea sativa Mill.) Shell Polyphenol Extract: A Valued Bioresource for Prostate Cancer Cell Growth Inhibition
title_sort untargeted characterization of chestnut (castanea sativa mill.) shell polyphenol extract: a valued bioresource for prostate cancer cell growth inhibition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357160/
https://www.ncbi.nlm.nih.gov/pubmed/32545546
http://dx.doi.org/10.3390/molecules25122730
work_keys_str_mv AT cacciolanunzioantonio untargetedcharacterizationofchestnutcastaneasativamillshellpolyphenolextractavaluedbioresourceforprostatecancercellgrowthinhibition
AT cerratoandrea untargetedcharacterizationofchestnutcastaneasativamillshellpolyphenolextractavaluedbioresourceforprostatecancercellgrowthinhibition
AT capriottiannalaura untargetedcharacterizationofchestnutcastaneasativamillshellpolyphenolextractavaluedbioresourceforprostatecancercellgrowthinhibition
AT cavalierechiara untargetedcharacterizationofchestnutcastaneasativamillshellpolyphenolextractavaluedbioresourceforprostatecancercellgrowthinhibition
AT dapolitomaria untargetedcharacterizationofchestnutcastaneasativamillshellpolyphenolextractavaluedbioresourceforprostatecancercellgrowthinhibition
AT montonecarmelamaria untargetedcharacterizationofchestnutcastaneasativamillshellpolyphenolextractavaluedbioresourceforprostatecancercellgrowthinhibition
AT piovesanasusy untargetedcharacterizationofchestnutcastaneasativamillshellpolyphenolextractavaluedbioresourceforprostatecancercellgrowthinhibition
AT squillacigiuseppe untargetedcharacterizationofchestnutcastaneasativamillshellpolyphenolextractavaluedbioresourceforprostatecancercellgrowthinhibition
AT pelusogianfranco untargetedcharacterizationofchestnutcastaneasativamillshellpolyphenolextractavaluedbioresourceforprostatecancercellgrowthinhibition
AT laganaaldo untargetedcharacterizationofchestnutcastaneasativamillshellpolyphenolextractavaluedbioresourceforprostatecancercellgrowthinhibition