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Estudio de las variantes alélicas CYP2C9*2 y CYP2C9*3 en muestras de población mestiza peruana
INTRODUCTION: CYP2C9 metabolizes approximately 15% of the prescribed drugs. Its gene has alleles whose frequencies differ between ethnic groups and populations. The alleles CYP2C9*2 and CYP2C9*3 account for an enzyme with decreased activity and their frequencies have not been determined in the Peruv...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Nacional de Salud
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357368/ https://www.ncbi.nlm.nih.gov/pubmed/31584773 http://dx.doi.org/10.7705/biomedica.4636 |
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author | Alvarado, Ángel Tito Muñoz, Ana María Loja, Berta Miyasato, Jessica Michiko García, Jorge Antonio Cerro, Roberto Andrés Quiñones, Luis Abel Varela, Nelson Miguel |
author_facet | Alvarado, Ángel Tito Muñoz, Ana María Loja, Berta Miyasato, Jessica Michiko García, Jorge Antonio Cerro, Roberto Andrés Quiñones, Luis Abel Varela, Nelson Miguel |
author_sort | Alvarado, Ángel Tito |
collection | PubMed |
description | INTRODUCTION: CYP2C9 metabolizes approximately 15% of the prescribed drugs. Its gene has alleles whose frequencies differ between ethnic groups and populations. The alleles CYP2C9*2 and CYP2C9*3 account for an enzyme with decreased activity and their frequencies have not been determined in the Peruvian mestizo population. OBJECTIVE: To characterize the frequencies of the allelic variants *2 (rs1799853) and *3 (rs1057910) of CYP2C9 gen in the Peruvian mestizo population from Lima, Tacna y Junín. MATERIALS AND METHODS: We conducted an observational, prospective cross-sectional study with non-probabilistic, by convenience, and incidental sampling. We included 218 subjects according to the inclusion and exclusion criteria, all of whom had signed the informed consent. We obtained the genomic DNA from oral mucosa swab. For the detection of the CYP2C9*2 and CYP2C9*3 genotypes, we used real-time-polymerase chain reaction with TaqMan® probes. RESULTS: The genotyping revealed that CYP2C9*2 and CYP2C9*3 variants have low frequencies (0.046 and 0.062, respectively). The frequency of intermediate metabolizers was 15.13% (CYP2C9*1/*2: 5.96%; CYP2C9*1/*3: 9.17%) and that of slow metabolizers was 3.22% (CYP2C9*2/*2: 1.38%; CYP2C9*3/*3: 1.38%; CYP2C9*2/*3: 0.46%). CONCLUSIONS: It was possible to determine the genotypic and allelic frequencies for the variants *2 and *3 of the CYP2C9 gene in a non-probabilistic sample of the Peruvian mestizo population. The frequencies obtained (0.046 and 0.062, respectively) corresponded to those expected for a South American mestizo population with Amerindian, European, African and Asian ancestry. |
format | Online Article Text |
id | pubmed-7357368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Instituto Nacional de Salud |
record_format | MEDLINE/PubMed |
spelling | pubmed-73573682020-07-20 Estudio de las variantes alélicas CYP2C9*2 y CYP2C9*3 en muestras de población mestiza peruana Alvarado, Ángel Tito Muñoz, Ana María Loja, Berta Miyasato, Jessica Michiko García, Jorge Antonio Cerro, Roberto Andrés Quiñones, Luis Abel Varela, Nelson Miguel Biomedica Comunicación Breve INTRODUCTION: CYP2C9 metabolizes approximately 15% of the prescribed drugs. Its gene has alleles whose frequencies differ between ethnic groups and populations. The alleles CYP2C9*2 and CYP2C9*3 account for an enzyme with decreased activity and their frequencies have not been determined in the Peruvian mestizo population. OBJECTIVE: To characterize the frequencies of the allelic variants *2 (rs1799853) and *3 (rs1057910) of CYP2C9 gen in the Peruvian mestizo population from Lima, Tacna y Junín. MATERIALS AND METHODS: We conducted an observational, prospective cross-sectional study with non-probabilistic, by convenience, and incidental sampling. We included 218 subjects according to the inclusion and exclusion criteria, all of whom had signed the informed consent. We obtained the genomic DNA from oral mucosa swab. For the detection of the CYP2C9*2 and CYP2C9*3 genotypes, we used real-time-polymerase chain reaction with TaqMan® probes. RESULTS: The genotyping revealed that CYP2C9*2 and CYP2C9*3 variants have low frequencies (0.046 and 0.062, respectively). The frequency of intermediate metabolizers was 15.13% (CYP2C9*1/*2: 5.96%; CYP2C9*1/*3: 9.17%) and that of slow metabolizers was 3.22% (CYP2C9*2/*2: 1.38%; CYP2C9*3/*3: 1.38%; CYP2C9*2/*3: 0.46%). CONCLUSIONS: It was possible to determine the genotypic and allelic frequencies for the variants *2 and *3 of the CYP2C9 gene in a non-probabilistic sample of the Peruvian mestizo population. The frequencies obtained (0.046 and 0.062, respectively) corresponded to those expected for a South American mestizo population with Amerindian, European, African and Asian ancestry. Instituto Nacional de Salud 2019-09-01 /pmc/articles/PMC7357368/ /pubmed/31584773 http://dx.doi.org/10.7705/biomedica.4636 Text en https://creativecommons.org/licenses/by/4.0/ Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons |
spellingShingle | Comunicación Breve Alvarado, Ángel Tito Muñoz, Ana María Loja, Berta Miyasato, Jessica Michiko García, Jorge Antonio Cerro, Roberto Andrés Quiñones, Luis Abel Varela, Nelson Miguel Estudio de las variantes alélicas CYP2C9*2 y CYP2C9*3 en muestras de población mestiza peruana |
title | Estudio de las variantes alélicas CYP2C9*2 y CYP2C9*3 en muestras de población mestiza peruana |
title_full | Estudio de las variantes alélicas CYP2C9*2 y CYP2C9*3 en muestras de población mestiza peruana |
title_fullStr | Estudio de las variantes alélicas CYP2C9*2 y CYP2C9*3 en muestras de población mestiza peruana |
title_full_unstemmed | Estudio de las variantes alélicas CYP2C9*2 y CYP2C9*3 en muestras de población mestiza peruana |
title_short | Estudio de las variantes alélicas CYP2C9*2 y CYP2C9*3 en muestras de población mestiza peruana |
title_sort | estudio de las variantes alélicas cyp2c9*2 y cyp2c9*3 en muestras de población mestiza peruana |
topic | Comunicación Breve |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357368/ https://www.ncbi.nlm.nih.gov/pubmed/31584773 http://dx.doi.org/10.7705/biomedica.4636 |
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