SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro

Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time rever...

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Detalles Bibliográficos
Autores principales: Cipitelli, Marcio da Costa, Valentin, Elizabeth, da Cruz, Nadia Vaez Gonçalves, Nogueira, Tatiana LS, de Melo, Elaine Cristina Amaro, da Silva, Rebeca Araujo, Serra, Marcelo M, Meriano, André L, Colares, Alberto ML, Dornelas-Ribeiro, Marcos, Santos, Caleb GM
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357545/
https://www.ncbi.nlm.nih.gov/pubmed/32667461
http://dx.doi.org/10.1590/0074-02760200200
Descripción
Sumario:Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.

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