SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro

Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time rever...

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Autores principales: Cipitelli, Marcio da Costa, Valentin, Elizabeth, da Cruz, Nadia Vaez Gonçalves, Nogueira, Tatiana LS, de Melo, Elaine Cristina Amaro, da Silva, Rebeca Araujo, Serra, Marcelo M, Meriano, André L, Colares, Alberto ML, Dornelas-Ribeiro, Marcos, Santos, Caleb GM
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357545/
https://www.ncbi.nlm.nih.gov/pubmed/32667461
http://dx.doi.org/10.1590/0074-02760200200
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author Cipitelli, Marcio da Costa
Valentin, Elizabeth
da Cruz, Nadia Vaez Gonçalves
Nogueira, Tatiana LS
de Melo, Elaine Cristina Amaro
da Silva, Rebeca Araujo
Serra, Marcelo M
Meriano, André L
Colares, Alberto ML
Dornelas-Ribeiro, Marcos
Santos, Caleb GM
author_facet Cipitelli, Marcio da Costa
Valentin, Elizabeth
da Cruz, Nadia Vaez Gonçalves
Nogueira, Tatiana LS
de Melo, Elaine Cristina Amaro
da Silva, Rebeca Araujo
Serra, Marcelo M
Meriano, André L
Colares, Alberto ML
Dornelas-Ribeiro, Marcos
Santos, Caleb GM
author_sort Cipitelli, Marcio da Costa
collection PubMed
description Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved.
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spelling pubmed-73575452020-07-21 SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro Cipitelli, Marcio da Costa Valentin, Elizabeth da Cruz, Nadia Vaez Gonçalves Nogueira, Tatiana LS de Melo, Elaine Cristina Amaro da Silva, Rebeca Araujo Serra, Marcelo M Meriano, André L Colares, Alberto ML Dornelas-Ribeiro, Marcos Santos, Caleb GM Mem Inst Oswaldo Cruz Short Communication Corona virus disease (COVID-19) presents a serious threat to global health. A historical timeline of early molecular diagnostics from government alert (January 22) (D) was presented. After in silico analysis, Brazilian Army Institute of Biology (IBEx-RJ) tested samples in house using real-time reverse transcriptase polymerase chain reaction (RT-PCR) (fast mode) based on Centers for Disease Control and Prevention (CDC) recommendations. First cases from Brazil, Rio de Janeiro, IBEx, and diagnosis team were reported in D36, D44, D66, and D74 respectively. Therefore, after 1300 tests, we recommend N1/N2 primer sets (CDC) for preliminary and Charité protocol confirmation in case of positive results. Moreover, every professional should be tested before starting work, in addition to weekly tests for everyone involved. Instituto Oswaldo Cruz, Ministério da Saúde 2020-07-13 /pmc/articles/PMC7357545/ /pubmed/32667461 http://dx.doi.org/10.1590/0074-02760200200 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
Cipitelli, Marcio da Costa
Valentin, Elizabeth
da Cruz, Nadia Vaez Gonçalves
Nogueira, Tatiana LS
de Melo, Elaine Cristina Amaro
da Silva, Rebeca Araujo
Serra, Marcelo M
Meriano, André L
Colares, Alberto ML
Dornelas-Ribeiro, Marcos
Santos, Caleb GM
SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro
title SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro
title_full SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro
title_fullStr SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro
title_full_unstemmed SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro
title_short SARS-CoV-2 diagnostic diary: from rumors to the first case. Early reports of molecular tests from the military research and diagnostic institute of Rio de Janeiro
title_sort sars-cov-2 diagnostic diary: from rumors to the first case. early reports of molecular tests from the military research and diagnostic institute of rio de janeiro
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357545/
https://www.ncbi.nlm.nih.gov/pubmed/32667461
http://dx.doi.org/10.1590/0074-02760200200
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