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First report of begomoviruses infecting Cucumis sativus L. in North America and identification of a proposed new begomovirus species

BACKGROUND: Members of the Begomovirus genus are phytopathogens that infect dicotyledonous plants, producing economic losses in tropical and subtropical regions. To date, only seven species of begomoviruses (BGVs) infecting cucumber have been described. Most cucumber infections were reported in Sout...

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Autores principales: Sanchez-Chavez, Sarahi, Regla-Marquez, Carlos Fernando, Cardenas-Conejo, Zugey Elizabeth, Garcia-Rodriguez, Daniel Alejandro, Centeno-Leija, Sara, Serrano-Posada, Hugo, Liñan-Rico, Andromeda, Partida-Palacios, Brenda Lizet, Cardenas-Conejo, Yair
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357562/
https://www.ncbi.nlm.nih.gov/pubmed/32728488
http://dx.doi.org/10.7717/peerj.9245
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author Sanchez-Chavez, Sarahi
Regla-Marquez, Carlos Fernando
Cardenas-Conejo, Zugey Elizabeth
Garcia-Rodriguez, Daniel Alejandro
Centeno-Leija, Sara
Serrano-Posada, Hugo
Liñan-Rico, Andromeda
Partida-Palacios, Brenda Lizet
Cardenas-Conejo, Yair
author_facet Sanchez-Chavez, Sarahi
Regla-Marquez, Carlos Fernando
Cardenas-Conejo, Zugey Elizabeth
Garcia-Rodriguez, Daniel Alejandro
Centeno-Leija, Sara
Serrano-Posada, Hugo
Liñan-Rico, Andromeda
Partida-Palacios, Brenda Lizet
Cardenas-Conejo, Yair
author_sort Sanchez-Chavez, Sarahi
collection PubMed
description BACKGROUND: Members of the Begomovirus genus are phytopathogens that infect dicotyledonous plants, producing economic losses in tropical and subtropical regions. To date, only seven species of begomoviruses (BGVs) infecting cucumber have been described. Most cucumber infections were reported in South Asia. In the Americas, begomoviral infections affecting cucumber are scarce; just one report of begomovirus has been described in South America. The presence of whitefly and typical symptoms of viral infections observed in a cucumber field in Colima, Mexico, suggested that plants in this field were affected by BGVs. METHODS: To identify the BGVs infecting cucumber, we performed a high-throughput sequencing and compared the assembled contigs against the GenBank nucleic acid sequence database. To confirm the presence of viruses in cucumber samples, we performed a PCR detection using specific oligonucleotides. We cloned and sequenced by Sanger method the complete genome of a potential new begomovirus. Begomovirus species demarcation was performed according to the International Committee on Taxonomy of Viruses. The evolutionary relationship of the new virus was inferred using phylogenetic and recombination analyses. RESULTS: We identified five species of begomovirus infecting plants in a field. None of these have been previously reported infecting cucumber. One of the five species of viruses here reported is a new begomovirus species. Cucumber chlorotic leaf virus, the new species, is a bipartite begomovirus that has distinctive features of viruses belonging to the squash leaf curl virus clade. CONCLUSIONS: The findings here described represent the first report of begomoviral infection affecting cucumber plants in North America. Previous to this report, only seven begomovirus species have been reported in the world, here we found five species infecting cucumber plants in a small sample suggesting that cucumber is vulnerable to BGVs. One of these viruses is a new species of begomovirus which is the first begomovirus originally isolated from the cucumber. The findings of this report could help to develop strategies to fight the begomoviral infections that affect cucumber crops.
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spelling pubmed-73575622020-07-28 First report of begomoviruses infecting Cucumis sativus L. in North America and identification of a proposed new begomovirus species Sanchez-Chavez, Sarahi Regla-Marquez, Carlos Fernando Cardenas-Conejo, Zugey Elizabeth Garcia-Rodriguez, Daniel Alejandro Centeno-Leija, Sara Serrano-Posada, Hugo Liñan-Rico, Andromeda Partida-Palacios, Brenda Lizet Cardenas-Conejo, Yair PeerJ Agricultural Science BACKGROUND: Members of the Begomovirus genus are phytopathogens that infect dicotyledonous plants, producing economic losses in tropical and subtropical regions. To date, only seven species of begomoviruses (BGVs) infecting cucumber have been described. Most cucumber infections were reported in South Asia. In the Americas, begomoviral infections affecting cucumber are scarce; just one report of begomovirus has been described in South America. The presence of whitefly and typical symptoms of viral infections observed in a cucumber field in Colima, Mexico, suggested that plants in this field were affected by BGVs. METHODS: To identify the BGVs infecting cucumber, we performed a high-throughput sequencing and compared the assembled contigs against the GenBank nucleic acid sequence database. To confirm the presence of viruses in cucumber samples, we performed a PCR detection using specific oligonucleotides. We cloned and sequenced by Sanger method the complete genome of a potential new begomovirus. Begomovirus species demarcation was performed according to the International Committee on Taxonomy of Viruses. The evolutionary relationship of the new virus was inferred using phylogenetic and recombination analyses. RESULTS: We identified five species of begomovirus infecting plants in a field. None of these have been previously reported infecting cucumber. One of the five species of viruses here reported is a new begomovirus species. Cucumber chlorotic leaf virus, the new species, is a bipartite begomovirus that has distinctive features of viruses belonging to the squash leaf curl virus clade. CONCLUSIONS: The findings here described represent the first report of begomoviral infection affecting cucumber plants in North America. Previous to this report, only seven begomovirus species have been reported in the world, here we found five species infecting cucumber plants in a small sample suggesting that cucumber is vulnerable to BGVs. One of these viruses is a new species of begomovirus which is the first begomovirus originally isolated from the cucumber. The findings of this report could help to develop strategies to fight the begomoviral infections that affect cucumber crops. PeerJ Inc. 2020-07-10 /pmc/articles/PMC7357562/ /pubmed/32728488 http://dx.doi.org/10.7717/peerj.9245 Text en © 2020 Sanchez-Chavez et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Sanchez-Chavez, Sarahi
Regla-Marquez, Carlos Fernando
Cardenas-Conejo, Zugey Elizabeth
Garcia-Rodriguez, Daniel Alejandro
Centeno-Leija, Sara
Serrano-Posada, Hugo
Liñan-Rico, Andromeda
Partida-Palacios, Brenda Lizet
Cardenas-Conejo, Yair
First report of begomoviruses infecting Cucumis sativus L. in North America and identification of a proposed new begomovirus species
title First report of begomoviruses infecting Cucumis sativus L. in North America and identification of a proposed new begomovirus species
title_full First report of begomoviruses infecting Cucumis sativus L. in North America and identification of a proposed new begomovirus species
title_fullStr First report of begomoviruses infecting Cucumis sativus L. in North America and identification of a proposed new begomovirus species
title_full_unstemmed First report of begomoviruses infecting Cucumis sativus L. in North America and identification of a proposed new begomovirus species
title_short First report of begomoviruses infecting Cucumis sativus L. in North America and identification of a proposed new begomovirus species
title_sort first report of begomoviruses infecting cucumis sativus l. in north america and identification of a proposed new begomovirus species
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357562/
https://www.ncbi.nlm.nih.gov/pubmed/32728488
http://dx.doi.org/10.7717/peerj.9245
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