Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling

Amine-reactive Tandem Mass Tag 10plex (TMT10plex) labeling permits multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS/MS). We have used this technology to label 20 Saccharomyces cerevisiae samples collected in a time-resolved manner from a wild-type and phos...

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Detalles Bibliográficos
Autores principales: Jones, Andrew W., Flynn, Helen R., Uhlmann, Frank, Snijders, Ambrosius P., Touati, Sandra A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357674/
https://www.ncbi.nlm.nih.gov/pubmed/32685930
http://dx.doi.org/10.1016/j.xpro.2020.100022
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author Jones, Andrew W.
Flynn, Helen R.
Uhlmann, Frank
Snijders, Ambrosius P.
Touati, Sandra A.
author_facet Jones, Andrew W.
Flynn, Helen R.
Uhlmann, Frank
Snijders, Ambrosius P.
Touati, Sandra A.
author_sort Jones, Andrew W.
collection PubMed
description Amine-reactive Tandem Mass Tag 10plex (TMT10plex) labeling permits multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS/MS). We have used this technology to label 20 Saccharomyces cerevisiae samples collected in a time-resolved manner from a wild-type and phosphatase mutant background to characterize phosphoproteome dynamics. Here, we provide a detailed protocol for biological and mass spectrometry sample preparation and analysis. For complete details on the use and execution of this protocol, please refer to Touati et al. (2019).
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spelling pubmed-73576742020-07-17 Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling Jones, Andrew W. Flynn, Helen R. Uhlmann, Frank Snijders, Ambrosius P. Touati, Sandra A. STAR Protoc Protocol Amine-reactive Tandem Mass Tag 10plex (TMT10plex) labeling permits multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS/MS). We have used this technology to label 20 Saccharomyces cerevisiae samples collected in a time-resolved manner from a wild-type and phosphatase mutant background to characterize phosphoproteome dynamics. Here, we provide a detailed protocol for biological and mass spectrometry sample preparation and analysis. For complete details on the use and execution of this protocol, please refer to Touati et al. (2019). Elsevier 2020-06-03 /pmc/articles/PMC7357674/ /pubmed/32685930 http://dx.doi.org/10.1016/j.xpro.2020.100022 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Jones, Andrew W.
Flynn, Helen R.
Uhlmann, Frank
Snijders, Ambrosius P.
Touati, Sandra A.
Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling
title Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling
title_full Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling
title_fullStr Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling
title_full_unstemmed Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling
title_short Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling
title_sort assessing budding yeast phosphoproteome dynamics in a time-resolved manner using tmt10plex mass tag labeling
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357674/
https://www.ncbi.nlm.nih.gov/pubmed/32685930
http://dx.doi.org/10.1016/j.xpro.2020.100022
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