Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

Applications of adenine base editors (ABEs) have been constrained by the limited compatibility of the deoxyadenosine deaminase component with Cas homologs other than SpCas9. We evolved the deaminase component of ABE7.10 using phage-assisted non-continuous and continuous evolution (PANCE and PACE), r...

Descripción completa

Detalles Bibliográficos
Autores principales: Richter, Michelle F., Zhao, Kevin T., Eton, Elliot, Lapinaite, Audrone, Newby, Gregory A., Thuronyi, Benjamin W., Wilson, Christopher, Koblan, Luke W., Zeng, Jing, Bauer, Daniel E., Doudna, Jennifer A., Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357821/
https://www.ncbi.nlm.nih.gov/pubmed/32433547
http://dx.doi.org/10.1038/s41587-020-0453-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357821/
https://www.ncbi.nlm.nih.gov/pubmed/32433547
http://dx.doi.org/10.1038/s41587-020-0453-z

Ejemplares similares