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Cellular Contact Guidance Emerges from Gap Avoidance
In the presence of anisotropic biochemical or topographical patterns, cells tend to align in the direction of these cues—a widely reported phenomenon known as “contact guidance.” To investigate the origins of contact guidance, here, we created substrates micropatterned with parallel lines of fibrone...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357833/ https://www.ncbi.nlm.nih.gov/pubmed/32685934 http://dx.doi.org/10.1016/j.xcrp.2020.100055 |
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author | Buskermolen, Antonetta B.C. Ristori, Tommaso Mostert, Dylan van Turnhout, Mark C. Shishvan, Siamak S. Loerakker, Sandra Kurniawan, Nicholas A. Deshpande, Vikram S. Bouten, Carlijn V.C. |
author_facet | Buskermolen, Antonetta B.C. Ristori, Tommaso Mostert, Dylan van Turnhout, Mark C. Shishvan, Siamak S. Loerakker, Sandra Kurniawan, Nicholas A. Deshpande, Vikram S. Bouten, Carlijn V.C. |
author_sort | Buskermolen, Antonetta B.C. |
collection | PubMed |
description | In the presence of anisotropic biochemical or topographical patterns, cells tend to align in the direction of these cues—a widely reported phenomenon known as “contact guidance.” To investigate the origins of contact guidance, here, we created substrates micropatterned with parallel lines of fibronectin with dimensions spanning multiple orders of magnitude. Quantitative morphometric analysis of our experimental data reveals two regimes of contact guidance governed by the length scale of the cues that cannot be explained by enforced alignment of focal adhesions. Adopting computational simulations of cell remodeling on inhomogeneous substrates based on a statistical mechanics framework for living cells, we show that contact guidance emerges from anisotropic cell shape fluctuation and “gap avoidance,” i.e., the energetic penalty of cell adhesions on non-adhesive gaps. Our findings therefore point to general biophysical mechanisms underlying cellular contact guidance, without the necessity of invoking specific molecular pathways. |
format | Online Article Text |
id | pubmed-7357833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-73578332020-07-17 Cellular Contact Guidance Emerges from Gap Avoidance Buskermolen, Antonetta B.C. Ristori, Tommaso Mostert, Dylan van Turnhout, Mark C. Shishvan, Siamak S. Loerakker, Sandra Kurniawan, Nicholas A. Deshpande, Vikram S. Bouten, Carlijn V.C. Cell Rep Phys Sci Article In the presence of anisotropic biochemical or topographical patterns, cells tend to align in the direction of these cues—a widely reported phenomenon known as “contact guidance.” To investigate the origins of contact guidance, here, we created substrates micropatterned with parallel lines of fibronectin with dimensions spanning multiple orders of magnitude. Quantitative morphometric analysis of our experimental data reveals two regimes of contact guidance governed by the length scale of the cues that cannot be explained by enforced alignment of focal adhesions. Adopting computational simulations of cell remodeling on inhomogeneous substrates based on a statistical mechanics framework for living cells, we show that contact guidance emerges from anisotropic cell shape fluctuation and “gap avoidance,” i.e., the energetic penalty of cell adhesions on non-adhesive gaps. Our findings therefore point to general biophysical mechanisms underlying cellular contact guidance, without the necessity of invoking specific molecular pathways. Cell Press 2020-05-20 /pmc/articles/PMC7357833/ /pubmed/32685934 http://dx.doi.org/10.1016/j.xcrp.2020.100055 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Buskermolen, Antonetta B.C. Ristori, Tommaso Mostert, Dylan van Turnhout, Mark C. Shishvan, Siamak S. Loerakker, Sandra Kurniawan, Nicholas A. Deshpande, Vikram S. Bouten, Carlijn V.C. Cellular Contact Guidance Emerges from Gap Avoidance |
title | Cellular Contact Guidance Emerges from Gap Avoidance |
title_full | Cellular Contact Guidance Emerges from Gap Avoidance |
title_fullStr | Cellular Contact Guidance Emerges from Gap Avoidance |
title_full_unstemmed | Cellular Contact Guidance Emerges from Gap Avoidance |
title_short | Cellular Contact Guidance Emerges from Gap Avoidance |
title_sort | cellular contact guidance emerges from gap avoidance |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7357833/ https://www.ncbi.nlm.nih.gov/pubmed/32685934 http://dx.doi.org/10.1016/j.xcrp.2020.100055 |
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