Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahy...

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Autores principales: Wang, Yangxin, Zhang, Ningning, Tan, Deming, Qi, Zhenhui, Wu, Changzhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358279/
https://www.ncbi.nlm.nih.gov/pubmed/32733866
http://dx.doi.org/10.3389/fbioe.2020.00714
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author Wang, Yangxin
Zhang, Ningning
Tan, Deming
Qi, Zhenhui
Wu, Changzhu
author_facet Wang, Yangxin
Zhang, Ningning
Tan, Deming
Qi, Zhenhui
Wu, Changzhu
author_sort Wang, Yangxin
collection PubMed
description In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO(3))(2)⋅6H(2)O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.
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spelling pubmed-73582792020-07-29 Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent Wang, Yangxin Zhang, Ningning Tan, Deming Qi, Zhenhui Wu, Changzhu Front Bioeng Biotechnol Bioengineering and Biotechnology In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO(3))(2)⋅6H(2)O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability. Frontiers Media S.A. 2020-07-07 /pmc/articles/PMC7358279/ /pubmed/32733866 http://dx.doi.org/10.3389/fbioe.2020.00714 Text en Copyright © 2020 Wang, Zhang, Tan, Qi and Wu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Wang, Yangxin
Zhang, Ningning
Tan, Deming
Qi, Zhenhui
Wu, Changzhu
Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
title Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
title_full Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
title_fullStr Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
title_full_unstemmed Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
title_short Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
title_sort facile synthesis of enzyme-embedded metal–organic frameworks for size-selective biocatalysis in organic solvent
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358279/
https://www.ncbi.nlm.nih.gov/pubmed/32733866
http://dx.doi.org/10.3389/fbioe.2020.00714
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