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Phylogenomic Classification and Biosynthetic Potential of the Fossil Fuel-Biodesulfurizing Rhodococcus Strain IGTS8

Rhodococcus strain IGTS8 is the most extensively studied model bacterium for biodesulfurization of fossil fuels via the non–destructive sulfur–specific 4S pathway. This strain was initially assigned to Rhodococcus rhodochrous and later to Rhodococcus erythropolis thus making its taxonomic status deb...

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Autores principales: Thompson, Dean, Cognat, Valérie, Goodfellow, Michael, Koechler, Sandrine, Heintz, Dimitri, Carapito, Christine, Van Dorsselaer, Alain, Mahmoud, Huda, Sangal, Vartul, Ismail, Wael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358434/
https://www.ncbi.nlm.nih.gov/pubmed/32733398
http://dx.doi.org/10.3389/fmicb.2020.01417
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author Thompson, Dean
Cognat, Valérie
Goodfellow, Michael
Koechler, Sandrine
Heintz, Dimitri
Carapito, Christine
Van Dorsselaer, Alain
Mahmoud, Huda
Sangal, Vartul
Ismail, Wael
author_facet Thompson, Dean
Cognat, Valérie
Goodfellow, Michael
Koechler, Sandrine
Heintz, Dimitri
Carapito, Christine
Van Dorsselaer, Alain
Mahmoud, Huda
Sangal, Vartul
Ismail, Wael
author_sort Thompson, Dean
collection PubMed
description Rhodococcus strain IGTS8 is the most extensively studied model bacterium for biodesulfurization of fossil fuels via the non–destructive sulfur–specific 4S pathway. This strain was initially assigned to Rhodococcus rhodochrous and later to Rhodococcus erythropolis thus making its taxonomic status debatable and reflecting the limited resolution of methods available at the time. In this study, phylogenomic analyses of the whole genome sequences of strain IGTS8 and closely related rhodococci showed that R. erythropolis and Rhodococcus qingshengii are very closely related species, that Rhodococcus strain IGTS8 is a R. qingshengii strain and that several strains identified as R. erythropolis should be re-classified as R. qingshengii. The genomes of strains assigned to these species contain potentially novel biosynthetic gene clusters showing that members of these taxa should be given greater importance in the search for new antimicrobials and other industrially important biomolecules. The plasmid-borne dsz operon encoding fossil fuel desulfurization enzymes was present in R. qingshengii IGTS8 and R. erythropolis XP suggesting that it might be transferable between members of these species.
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spelling pubmed-73584342020-07-29 Phylogenomic Classification and Biosynthetic Potential of the Fossil Fuel-Biodesulfurizing Rhodococcus Strain IGTS8 Thompson, Dean Cognat, Valérie Goodfellow, Michael Koechler, Sandrine Heintz, Dimitri Carapito, Christine Van Dorsselaer, Alain Mahmoud, Huda Sangal, Vartul Ismail, Wael Front Microbiol Microbiology Rhodococcus strain IGTS8 is the most extensively studied model bacterium for biodesulfurization of fossil fuels via the non–destructive sulfur–specific 4S pathway. This strain was initially assigned to Rhodococcus rhodochrous and later to Rhodococcus erythropolis thus making its taxonomic status debatable and reflecting the limited resolution of methods available at the time. In this study, phylogenomic analyses of the whole genome sequences of strain IGTS8 and closely related rhodococci showed that R. erythropolis and Rhodococcus qingshengii are very closely related species, that Rhodococcus strain IGTS8 is a R. qingshengii strain and that several strains identified as R. erythropolis should be re-classified as R. qingshengii. The genomes of strains assigned to these species contain potentially novel biosynthetic gene clusters showing that members of these taxa should be given greater importance in the search for new antimicrobials and other industrially important biomolecules. The plasmid-borne dsz operon encoding fossil fuel desulfurization enzymes was present in R. qingshengii IGTS8 and R. erythropolis XP suggesting that it might be transferable between members of these species. Frontiers Media S.A. 2020-07-07 /pmc/articles/PMC7358434/ /pubmed/32733398 http://dx.doi.org/10.3389/fmicb.2020.01417 Text en Copyright © 2020 Thompson, Cognat, Goodfellow, Koechler, Heintz, Carapito, Van Dorsselaer, Mahmoud, Sangal and Ismail. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Thompson, Dean
Cognat, Valérie
Goodfellow, Michael
Koechler, Sandrine
Heintz, Dimitri
Carapito, Christine
Van Dorsselaer, Alain
Mahmoud, Huda
Sangal, Vartul
Ismail, Wael
Phylogenomic Classification and Biosynthetic Potential of the Fossil Fuel-Biodesulfurizing Rhodococcus Strain IGTS8
title Phylogenomic Classification and Biosynthetic Potential of the Fossil Fuel-Biodesulfurizing Rhodococcus Strain IGTS8
title_full Phylogenomic Classification and Biosynthetic Potential of the Fossil Fuel-Biodesulfurizing Rhodococcus Strain IGTS8
title_fullStr Phylogenomic Classification and Biosynthetic Potential of the Fossil Fuel-Biodesulfurizing Rhodococcus Strain IGTS8
title_full_unstemmed Phylogenomic Classification and Biosynthetic Potential of the Fossil Fuel-Biodesulfurizing Rhodococcus Strain IGTS8
title_short Phylogenomic Classification and Biosynthetic Potential of the Fossil Fuel-Biodesulfurizing Rhodococcus Strain IGTS8
title_sort phylogenomic classification and biosynthetic potential of the fossil fuel-biodesulfurizing rhodococcus strain igts8
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358434/
https://www.ncbi.nlm.nih.gov/pubmed/32733398
http://dx.doi.org/10.3389/fmicb.2020.01417
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