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Microbiome Variation Across Two Hemlock Species With Hemlock Woolly Adelgid Infestation

The hemlock woolly adelgid (Adelges tsugae, HWA), an invasive insect, is devastating native hemlock populations in eastern North America, and management outcomes have so far had limited success. While many plant microbiomes influence and even support plant immune responses to insect herbivory, relat...

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Autores principales: Dove, Nicholas C., Rogers, Timothy J., Leppanen, Christy, Simberloff, Daniel, Fordyce, James A., Brown, Veronica A., LeBude, Anthony V., Ranney, Thomas G., Cregger, Melissa A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358439/
https://www.ncbi.nlm.nih.gov/pubmed/32733417
http://dx.doi.org/10.3389/fmicb.2020.01528
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author Dove, Nicholas C.
Rogers, Timothy J.
Leppanen, Christy
Simberloff, Daniel
Fordyce, James A.
Brown, Veronica A.
LeBude, Anthony V.
Ranney, Thomas G.
Cregger, Melissa A.
author_facet Dove, Nicholas C.
Rogers, Timothy J.
Leppanen, Christy
Simberloff, Daniel
Fordyce, James A.
Brown, Veronica A.
LeBude, Anthony V.
Ranney, Thomas G.
Cregger, Melissa A.
author_sort Dove, Nicholas C.
collection PubMed
description The hemlock woolly adelgid (Adelges tsugae, HWA), an invasive insect, is devastating native hemlock populations in eastern North America, and management outcomes have so far had limited success. While many plant microbiomes influence and even support plant immune responses to insect herbivory, relatively little is known about the hemlock microbiome and its interactions with pathogens or herbivores such as HWA. Using 16S rRNA and ITS gene amplicon sequencing, we characterized the needle, branch, root, and rhizosphere microbiome of two hemlock species, Tsuga canadensis and T. sieboldii, that displayed low and high levels of HWA populations. We found that both archaeal/bacterial and fungal needle communities, as well as the archaeal/bacterial branch and root communities, varied in composition in both hemlock species relative to HWA population levels. While host species and plant-associated habitats explained a greater proportion of the variance in the microbiome than did HWA population level, high HWA populations were associated with enrichment of 100 likely fungal pathogen sequence variants across the four plant-associated habitats (e.g., needle, branch, root, rhizosphere) compared to trees with lower HWA populations. This work contributes to a growing body of literature linking plant pathogens and pests with the changes in the associated plant microbiome and host health. Furthermore, this work demonstrates the need to further investigate plant microbiome effects across multiple plant tissues to understand their influences on host health.
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spelling pubmed-73584392020-07-29 Microbiome Variation Across Two Hemlock Species With Hemlock Woolly Adelgid Infestation Dove, Nicholas C. Rogers, Timothy J. Leppanen, Christy Simberloff, Daniel Fordyce, James A. Brown, Veronica A. LeBude, Anthony V. Ranney, Thomas G. Cregger, Melissa A. Front Microbiol Microbiology The hemlock woolly adelgid (Adelges tsugae, HWA), an invasive insect, is devastating native hemlock populations in eastern North America, and management outcomes have so far had limited success. While many plant microbiomes influence and even support plant immune responses to insect herbivory, relatively little is known about the hemlock microbiome and its interactions with pathogens or herbivores such as HWA. Using 16S rRNA and ITS gene amplicon sequencing, we characterized the needle, branch, root, and rhizosphere microbiome of two hemlock species, Tsuga canadensis and T. sieboldii, that displayed low and high levels of HWA populations. We found that both archaeal/bacterial and fungal needle communities, as well as the archaeal/bacterial branch and root communities, varied in composition in both hemlock species relative to HWA population levels. While host species and plant-associated habitats explained a greater proportion of the variance in the microbiome than did HWA population level, high HWA populations were associated with enrichment of 100 likely fungal pathogen sequence variants across the four plant-associated habitats (e.g., needle, branch, root, rhizosphere) compared to trees with lower HWA populations. This work contributes to a growing body of literature linking plant pathogens and pests with the changes in the associated plant microbiome and host health. Furthermore, this work demonstrates the need to further investigate plant microbiome effects across multiple plant tissues to understand their influences on host health. Frontiers Media S.A. 2020-07-07 /pmc/articles/PMC7358439/ /pubmed/32733417 http://dx.doi.org/10.3389/fmicb.2020.01528 Text en Copyright © 2020 Dove, Rogers, Leppanen, Simberloff, Fordyce, Brown, LeBude, Ranney and Cregger. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Dove, Nicholas C.
Rogers, Timothy J.
Leppanen, Christy
Simberloff, Daniel
Fordyce, James A.
Brown, Veronica A.
LeBude, Anthony V.
Ranney, Thomas G.
Cregger, Melissa A.
Microbiome Variation Across Two Hemlock Species With Hemlock Woolly Adelgid Infestation
title Microbiome Variation Across Two Hemlock Species With Hemlock Woolly Adelgid Infestation
title_full Microbiome Variation Across Two Hemlock Species With Hemlock Woolly Adelgid Infestation
title_fullStr Microbiome Variation Across Two Hemlock Species With Hemlock Woolly Adelgid Infestation
title_full_unstemmed Microbiome Variation Across Two Hemlock Species With Hemlock Woolly Adelgid Infestation
title_short Microbiome Variation Across Two Hemlock Species With Hemlock Woolly Adelgid Infestation
title_sort microbiome variation across two hemlock species with hemlock woolly adelgid infestation
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358439/
https://www.ncbi.nlm.nih.gov/pubmed/32733417
http://dx.doi.org/10.3389/fmicb.2020.01528
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