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Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue
Advances in neural engineering have brought about a number of implantable devices for improved brain stimulation and recording. Unfortunately, many of these micro-implants have not been adopted due to issues of signal loss, deterioration, and host response to the device. While glial scar characteriz...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358524/ https://www.ncbi.nlm.nih.gov/pubmed/32733179 http://dx.doi.org/10.3389/fnins.2020.00095 |
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author | Esquibel, Corinne R. Wendt, Kristy D. Lee, Heui C. Gaire, Janak Shoffstall, Andrew Urdaneta, Morgan E. Chacko, Jenu V. Brodnick, Sarah K. Otto, Kevin J. Capadona, Jeffrey R. Williams, Justin C. Eliceiri, K. W. |
author_facet | Esquibel, Corinne R. Wendt, Kristy D. Lee, Heui C. Gaire, Janak Shoffstall, Andrew Urdaneta, Morgan E. Chacko, Jenu V. Brodnick, Sarah K. Otto, Kevin J. Capadona, Jeffrey R. Williams, Justin C. Eliceiri, K. W. |
author_sort | Esquibel, Corinne R. |
collection | PubMed |
description | Advances in neural engineering have brought about a number of implantable devices for improved brain stimulation and recording. Unfortunately, many of these micro-implants have not been adopted due to issues of signal loss, deterioration, and host response to the device. While glial scar characterization is critical to better understand the mechanisms that affect device functionality or tissue viability, analysis is frequently hindered by immunohistochemical tissue processing methods that result in device shattering and tissue tearing artifacts. Devices are commonly removed prior to sectioning, which can itself disturb the quality of the study. In this methods implementation study, we use the label free, optical sectioning method of second harmonic generation (SHG) to examine brain slices of various implanted intracortical electrodes and demonstrate collagen fiber distribution not found in normal brain tissue. SHG can easily be used in conjunction with multiphoton microscopy to allow direct intrinsic visualization of collagen-containing glial scars on the surface of cortically implanted electrode probes without imposing the physical strain of tissue sectioning methods required for other high resolution light microscopy modalities. Identification and future measurements of these collagen fibers may be useful in predicting host immune response and device signal fidelity. |
format | Online Article Text |
id | pubmed-7358524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73585242020-07-29 Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue Esquibel, Corinne R. Wendt, Kristy D. Lee, Heui C. Gaire, Janak Shoffstall, Andrew Urdaneta, Morgan E. Chacko, Jenu V. Brodnick, Sarah K. Otto, Kevin J. Capadona, Jeffrey R. Williams, Justin C. Eliceiri, K. W. Front Neurosci Neuroscience Advances in neural engineering have brought about a number of implantable devices for improved brain stimulation and recording. Unfortunately, many of these micro-implants have not been adopted due to issues of signal loss, deterioration, and host response to the device. While glial scar characterization is critical to better understand the mechanisms that affect device functionality or tissue viability, analysis is frequently hindered by immunohistochemical tissue processing methods that result in device shattering and tissue tearing artifacts. Devices are commonly removed prior to sectioning, which can itself disturb the quality of the study. In this methods implementation study, we use the label free, optical sectioning method of second harmonic generation (SHG) to examine brain slices of various implanted intracortical electrodes and demonstrate collagen fiber distribution not found in normal brain tissue. SHG can easily be used in conjunction with multiphoton microscopy to allow direct intrinsic visualization of collagen-containing glial scars on the surface of cortically implanted electrode probes without imposing the physical strain of tissue sectioning methods required for other high resolution light microscopy modalities. Identification and future measurements of these collagen fibers may be useful in predicting host immune response and device signal fidelity. Frontiers Media S.A. 2020-07-07 /pmc/articles/PMC7358524/ /pubmed/32733179 http://dx.doi.org/10.3389/fnins.2020.00095 Text en Copyright © 2020 Esquibel, Wendt, Lee, Gaire, Shoffstall, Urdaneta, Chacko, Brodnick, Otto, Capadona, Williams and Eliceiri. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Esquibel, Corinne R. Wendt, Kristy D. Lee, Heui C. Gaire, Janak Shoffstall, Andrew Urdaneta, Morgan E. Chacko, Jenu V. Brodnick, Sarah K. Otto, Kevin J. Capadona, Jeffrey R. Williams, Justin C. Eliceiri, K. W. Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue |
title | Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue |
title_full | Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue |
title_fullStr | Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue |
title_full_unstemmed | Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue |
title_short | Second Harmonic Generation Imaging of Collagen in Chronically Implantable Electrodes in Brain Tissue |
title_sort | second harmonic generation imaging of collagen in chronically implantable electrodes in brain tissue |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358524/ https://www.ncbi.nlm.nih.gov/pubmed/32733179 http://dx.doi.org/10.3389/fnins.2020.00095 |
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