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Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis
BACKGROUND: Osteosarcoma (OS) is the most common bone malignant tumor in children, youth, and adolescents. Circular RNA hsa_circ_0005909 (circ_0005909) is involved in the progression of OS. Nevertheless, there are few reports on the role and mechanism of circ_0005909 in OS. METHODS: Quantitative rea...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359231/ https://www.ncbi.nlm.nih.gov/pubmed/32684842 http://dx.doi.org/10.1186/s12935-020-01399-1 |
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author | Ding, Shuai Zhang, Guangquan Gao, Yanzheng Chen, Shulian Cao, Chen |
author_facet | Ding, Shuai Zhang, Guangquan Gao, Yanzheng Chen, Shulian Cao, Chen |
author_sort | Ding, Shuai |
collection | PubMed |
description | BACKGROUND: Osteosarcoma (OS) is the most common bone malignant tumor in children, youth, and adolescents. Circular RNA hsa_circ_0005909 (circ_0005909) is involved in the progression of OS. Nevertheless, there are few reports on the role and mechanism of circ_0005909 in OS. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was executed to examine the expression of circ_0005909, miR-936, and High Mobility Group Box 1 (HMGB1) mRNA in OS tissues and cells. Cell viability, colony formation, migration, and invasion were evaluated by Cell Counting Kit-8 (CCK-8), cell colony formation, or transwell assays. Cell epithelial-mesenchymal transition (EMT) and HMGB1 protein levels were assessed through western blot analysis. The role of circ_0005909 on tumor growth in vivo was verified by xenograft assay. The relationship between circ_0005909 or HMGB1 and miR-936 was confirmed with the dual-luciferase reporter or RNA pull-down assays. RESULTS: Circ_0005909 level was upregulated in OS tissues and cells. OS patients with high circ_0005909 expression had a lower survival rate. Circ_0005909 inhibition reduced tumor growth in vivo and constrained cell viability, colony formation, migration, invasion, and EMT of OS cells in vitro. Furthermore, circ_0005909 served as a sponge for miR-936 and the repressive impacts of circ_0005909 silencing on malignant behaviors of OS cells were abolished by miR-936 inhibitors. Also, HMGB1 acted as a target for miR-936 and was modulated by circ_0005909 via miR-936. Additionally, HMGB1 overexpression restored the inhibitory influence on the malignant behaviors of OS cells mediated by circ_0005909 inhibition. CONCLUSIONS: Circ_0005909 inhibition impeded the progression of OS via downregulating HMGB1 via sponging miR-936. |
format | Online Article Text |
id | pubmed-7359231 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-73592312020-07-17 Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis Ding, Shuai Zhang, Guangquan Gao, Yanzheng Chen, Shulian Cao, Chen Cancer Cell Int Primary Research BACKGROUND: Osteosarcoma (OS) is the most common bone malignant tumor in children, youth, and adolescents. Circular RNA hsa_circ_0005909 (circ_0005909) is involved in the progression of OS. Nevertheless, there are few reports on the role and mechanism of circ_0005909 in OS. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was executed to examine the expression of circ_0005909, miR-936, and High Mobility Group Box 1 (HMGB1) mRNA in OS tissues and cells. Cell viability, colony formation, migration, and invasion were evaluated by Cell Counting Kit-8 (CCK-8), cell colony formation, or transwell assays. Cell epithelial-mesenchymal transition (EMT) and HMGB1 protein levels were assessed through western blot analysis. The role of circ_0005909 on tumor growth in vivo was verified by xenograft assay. The relationship between circ_0005909 or HMGB1 and miR-936 was confirmed with the dual-luciferase reporter or RNA pull-down assays. RESULTS: Circ_0005909 level was upregulated in OS tissues and cells. OS patients with high circ_0005909 expression had a lower survival rate. Circ_0005909 inhibition reduced tumor growth in vivo and constrained cell viability, colony formation, migration, invasion, and EMT of OS cells in vitro. Furthermore, circ_0005909 served as a sponge for miR-936 and the repressive impacts of circ_0005909 silencing on malignant behaviors of OS cells were abolished by miR-936 inhibitors. Also, HMGB1 acted as a target for miR-936 and was modulated by circ_0005909 via miR-936. Additionally, HMGB1 overexpression restored the inhibitory influence on the malignant behaviors of OS cells mediated by circ_0005909 inhibition. CONCLUSIONS: Circ_0005909 inhibition impeded the progression of OS via downregulating HMGB1 via sponging miR-936. BioMed Central 2020-07-13 /pmc/articles/PMC7359231/ /pubmed/32684842 http://dx.doi.org/10.1186/s12935-020-01399-1 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Primary Research Ding, Shuai Zhang, Guangquan Gao, Yanzheng Chen, Shulian Cao, Chen Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis |
title | Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis |
title_full | Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis |
title_fullStr | Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis |
title_full_unstemmed | Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis |
title_short | Circular RNA hsa_circ_0005909 modulates osteosarcoma progression via the miR-936/HMGB1 axis |
title_sort | circular rna hsa_circ_0005909 modulates osteosarcoma progression via the mir-936/hmgb1 axis |
topic | Primary Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359231/ https://www.ncbi.nlm.nih.gov/pubmed/32684842 http://dx.doi.org/10.1186/s12935-020-01399-1 |
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