Depletion of microglia exacerbates injury and impairs function recovery after spinal cord injury in mice

The role of microglia in spinal cord injury (SCI) remains ambiguous, partially due to the paucity of efficient methods to discriminate these resident microglia with blood-derived monocytes/macrophages. Here, we used pharmacological treatments to specifically eliminate microglia and subsequently to i...

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Autores principales: Fu, Haitao, Zhao, Yanpeng, Hu, Die, Wang, Song, Yu, Tengbo, Zhang, Licheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359318/
https://www.ncbi.nlm.nih.gov/pubmed/32661227
http://dx.doi.org/10.1038/s41419-020-2733-4
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author Fu, Haitao
Zhao, Yanpeng
Hu, Die
Wang, Song
Yu, Tengbo
Zhang, Licheng
author_facet Fu, Haitao
Zhao, Yanpeng
Hu, Die
Wang, Song
Yu, Tengbo
Zhang, Licheng
author_sort Fu, Haitao
collection PubMed
description The role of microglia in spinal cord injury (SCI) remains ambiguous, partially due to the paucity of efficient methods to discriminate these resident microglia with blood-derived monocytes/macrophages. Here, we used pharmacological treatments to specifically eliminate microglia and subsequently to investigate the response of microglia after SCI in mice. We showed that treatment with colony stimulating factor 1 receptor (CSF1R) inhibitor PLX3397 eliminated ~90% microglia and did not affect other cell types in mouse spinal cord. PLX3397 treatment also induced a strong decrease in microglial proliferation induced by SCI. Depletion of microglia after SCI disrupted glial scar formation, enhanced immune cell infiltrates, reduced neuronal survival, delayed astrocyte repopulation, exacerbated axonal dieback, and impaired locomotor recovery. Therefore, our findings suggest microglia may play a protective role after SCI in mice.
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spelling pubmed-73593182020-07-20 Depletion of microglia exacerbates injury and impairs function recovery after spinal cord injury in mice Fu, Haitao Zhao, Yanpeng Hu, Die Wang, Song Yu, Tengbo Zhang, Licheng Cell Death Dis Article The role of microglia in spinal cord injury (SCI) remains ambiguous, partially due to the paucity of efficient methods to discriminate these resident microglia with blood-derived monocytes/macrophages. Here, we used pharmacological treatments to specifically eliminate microglia and subsequently to investigate the response of microglia after SCI in mice. We showed that treatment with colony stimulating factor 1 receptor (CSF1R) inhibitor PLX3397 eliminated ~90% microglia and did not affect other cell types in mouse spinal cord. PLX3397 treatment also induced a strong decrease in microglial proliferation induced by SCI. Depletion of microglia after SCI disrupted glial scar formation, enhanced immune cell infiltrates, reduced neuronal survival, delayed astrocyte repopulation, exacerbated axonal dieback, and impaired locomotor recovery. Therefore, our findings suggest microglia may play a protective role after SCI in mice. Nature Publishing Group UK 2020-07-13 /pmc/articles/PMC7359318/ /pubmed/32661227 http://dx.doi.org/10.1038/s41419-020-2733-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Fu, Haitao
Zhao, Yanpeng
Hu, Die
Wang, Song
Yu, Tengbo
Zhang, Licheng
Depletion of microglia exacerbates injury and impairs function recovery after spinal cord injury in mice
title Depletion of microglia exacerbates injury and impairs function recovery after spinal cord injury in mice
title_full Depletion of microglia exacerbates injury and impairs function recovery after spinal cord injury in mice
title_fullStr Depletion of microglia exacerbates injury and impairs function recovery after spinal cord injury in mice
title_full_unstemmed Depletion of microglia exacerbates injury and impairs function recovery after spinal cord injury in mice
title_short Depletion of microglia exacerbates injury and impairs function recovery after spinal cord injury in mice
title_sort depletion of microglia exacerbates injury and impairs function recovery after spinal cord injury in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359318/
https://www.ncbi.nlm.nih.gov/pubmed/32661227
http://dx.doi.org/10.1038/s41419-020-2733-4
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