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Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription
Sperm contributes genetic and epigenetic information to the embryo to efficiently support development. However, the mechanism underlying such developmental competence remains elusive. Here, we investigated whether all sperm cells have a common epigenetic configuration that primes transcriptional pro...
| Autores principales: | , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2020
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359334/ https://www.ncbi.nlm.nih.gov/pubmed/32661239 http://dx.doi.org/10.1038/s41467-020-17238-w |
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| author | Oikawa, Mami Simeone, Angela Hormanseder, Eva Teperek, Marta Gaggioli, Vincent O’Doherty, Alan Falk, Emma Sporniak, Matthieu D’Santos, Clive Franklin, Valar Nila Roamio Kishore, Kamal Bradshaw, Charles R. Keane, Declan Freour, Thomas David, Laurent Grzybowski, Adrian T. Ruthenburg, Alexander J. Gurdon, John Jullien, Jerome |
| author_facet | Oikawa, Mami Simeone, Angela Hormanseder, Eva Teperek, Marta Gaggioli, Vincent O’Doherty, Alan Falk, Emma Sporniak, Matthieu D’Santos, Clive Franklin, Valar Nila Roamio Kishore, Kamal Bradshaw, Charles R. Keane, Declan Freour, Thomas David, Laurent Grzybowski, Adrian T. Ruthenburg, Alexander J. Gurdon, John Jullien, Jerome |
| author_sort | Oikawa, Mami |
| collection | PubMed |
| description | Sperm contributes genetic and epigenetic information to the embryo to efficiently support development. However, the mechanism underlying such developmental competence remains elusive. Here, we investigated whether all sperm cells have a common epigenetic configuration that primes transcriptional program for embryonic development. Using calibrated ChIP-seq, we show that remodelling of histones during spermiogenesis results in the retention of methylated histone H3 at the same genomic location in most sperm cell. This homogeneously methylated fraction of histone H3 in the sperm genome is maintained during early embryonic replication. Such methylated histone fraction resisting post-fertilisation reprogramming marks developmental genes whose expression is perturbed upon experimental reduction of histone methylation. A similar homogeneously methylated histone H3 fraction is detected in human sperm. Altogether, we uncover a conserved mechanism of paternal epigenetic information transmission to the embryo through the homogeneous retention of methylated histone in a sperm cells population. |
| format | Online Article Text |
| id | pubmed-7359334 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-73593342020-07-20 Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription Oikawa, Mami Simeone, Angela Hormanseder, Eva Teperek, Marta Gaggioli, Vincent O’Doherty, Alan Falk, Emma Sporniak, Matthieu D’Santos, Clive Franklin, Valar Nila Roamio Kishore, Kamal Bradshaw, Charles R. Keane, Declan Freour, Thomas David, Laurent Grzybowski, Adrian T. Ruthenburg, Alexander J. Gurdon, John Jullien, Jerome Nat Commun Article Sperm contributes genetic and epigenetic information to the embryo to efficiently support development. However, the mechanism underlying such developmental competence remains elusive. Here, we investigated whether all sperm cells have a common epigenetic configuration that primes transcriptional program for embryonic development. Using calibrated ChIP-seq, we show that remodelling of histones during spermiogenesis results in the retention of methylated histone H3 at the same genomic location in most sperm cell. This homogeneously methylated fraction of histone H3 in the sperm genome is maintained during early embryonic replication. Such methylated histone fraction resisting post-fertilisation reprogramming marks developmental genes whose expression is perturbed upon experimental reduction of histone methylation. A similar homogeneously methylated histone H3 fraction is detected in human sperm. Altogether, we uncover a conserved mechanism of paternal epigenetic information transmission to the embryo through the homogeneous retention of methylated histone in a sperm cells population. Nature Publishing Group UK 2020-07-13 /pmc/articles/PMC7359334/ /pubmed/32661239 http://dx.doi.org/10.1038/s41467-020-17238-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
| spellingShingle | Article Oikawa, Mami Simeone, Angela Hormanseder, Eva Teperek, Marta Gaggioli, Vincent O’Doherty, Alan Falk, Emma Sporniak, Matthieu D’Santos, Clive Franklin, Valar Nila Roamio Kishore, Kamal Bradshaw, Charles R. Keane, Declan Freour, Thomas David, Laurent Grzybowski, Adrian T. Ruthenburg, Alexander J. Gurdon, John Jullien, Jerome Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription |
| title | Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription |
| title_full | Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription |
| title_fullStr | Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription |
| title_full_unstemmed | Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription |
| title_short | Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription |
| title_sort | epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359334/ https://www.ncbi.nlm.nih.gov/pubmed/32661239 http://dx.doi.org/10.1038/s41467-020-17238-w |
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