Deep three-photon imaging of the brain in intact adult zebrafish

Behaviors emerge from activity throughout the brain, but non-invasive optical access in adult vertebrate brains is limited. We show that three-photon (3P) imaging through the head of intact adult zebrafish allows structural and functional imaging at cellular resolution throughout the telencephalon a...

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Autores principales: Chow, Dawnis M., Sinefeld, David, Kolkman, Kristine E., Ouzounov, Dimitre G., Akbari, Najva, Tatarsky, Rose, Bass, Andrew, Xu, Chris, Fetcho, Joseph R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359951/
https://www.ncbi.nlm.nih.gov/pubmed/32341543
http://dx.doi.org/10.1038/s41592-020-0819-7
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author Chow, Dawnis M.
Sinefeld, David
Kolkman, Kristine E.
Ouzounov, Dimitre G.
Akbari, Najva
Tatarsky, Rose
Bass, Andrew
Xu, Chris
Fetcho, Joseph R.
author_facet Chow, Dawnis M.
Sinefeld, David
Kolkman, Kristine E.
Ouzounov, Dimitre G.
Akbari, Najva
Tatarsky, Rose
Bass, Andrew
Xu, Chris
Fetcho, Joseph R.
author_sort Chow, Dawnis M.
collection PubMed
description Behaviors emerge from activity throughout the brain, but non-invasive optical access in adult vertebrate brains is limited. We show that three-photon (3P) imaging through the head of intact adult zebrafish allows structural and functional imaging at cellular resolution throughout the telencephalon and deep into the cerebellum and optic tectum. With 3P imaging, considerable portions of the brain become non-invasively accessible from embryo to sexually mature adult in a vertebrate model.
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spelling pubmed-73599512020-10-27 Deep three-photon imaging of the brain in intact adult zebrafish Chow, Dawnis M. Sinefeld, David Kolkman, Kristine E. Ouzounov, Dimitre G. Akbari, Najva Tatarsky, Rose Bass, Andrew Xu, Chris Fetcho, Joseph R. Nat Methods Article Behaviors emerge from activity throughout the brain, but non-invasive optical access in adult vertebrate brains is limited. We show that three-photon (3P) imaging through the head of intact adult zebrafish allows structural and functional imaging at cellular resolution throughout the telencephalon and deep into the cerebellum and optic tectum. With 3P imaging, considerable portions of the brain become non-invasively accessible from embryo to sexually mature adult in a vertebrate model. 2020-04-27 2020-06 /pmc/articles/PMC7359951/ /pubmed/32341543 http://dx.doi.org/10.1038/s41592-020-0819-7 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Chow, Dawnis M.
Sinefeld, David
Kolkman, Kristine E.
Ouzounov, Dimitre G.
Akbari, Najva
Tatarsky, Rose
Bass, Andrew
Xu, Chris
Fetcho, Joseph R.
Deep three-photon imaging of the brain in intact adult zebrafish
title Deep three-photon imaging of the brain in intact adult zebrafish
title_full Deep three-photon imaging of the brain in intact adult zebrafish
title_fullStr Deep three-photon imaging of the brain in intact adult zebrafish
title_full_unstemmed Deep three-photon imaging of the brain in intact adult zebrafish
title_short Deep three-photon imaging of the brain in intact adult zebrafish
title_sort deep three-photon imaging of the brain in intact adult zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7359951/
https://www.ncbi.nlm.nih.gov/pubmed/32341543
http://dx.doi.org/10.1038/s41592-020-0819-7
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