Albumin-Modified Melanin-Silica Hybrid Nanoparticles Target Breast Cancer Cells via a SPARC-Dependent Mechanism

Bioconjugation of a recently developed photoacoustic nanoprobe, based on silica-templated eumelanin-silver hybrid nanoparticles (MelaSil_Ag-NPs), with human serum albumin (HSA) is disclosed herein as an efficient and practical strategy to improve photostability and to perform SPARC mediated internal...

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Autores principales: Sanità, Gennaro, Armanetti, Paolo, Silvestri, Brigida, Carrese, Barbara, Calì, Gaetano, Pota, Giulio, Pezzella, Alessandro, d’Ischia, Marco, Luciani, Giuseppina, Menichetti, Luca, Lamberti, Annalisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360861/
https://www.ncbi.nlm.nih.gov/pubmed/32733871
http://dx.doi.org/10.3389/fbioe.2020.00765
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author Sanità, Gennaro
Armanetti, Paolo
Silvestri, Brigida
Carrese, Barbara
Calì, Gaetano
Pota, Giulio
Pezzella, Alessandro
d’Ischia, Marco
Luciani, Giuseppina
Menichetti, Luca
Lamberti, Annalisa
author_facet Sanità, Gennaro
Armanetti, Paolo
Silvestri, Brigida
Carrese, Barbara
Calì, Gaetano
Pota, Giulio
Pezzella, Alessandro
d’Ischia, Marco
Luciani, Giuseppina
Menichetti, Luca
Lamberti, Annalisa
author_sort Sanità, Gennaro
collection PubMed
description Bioconjugation of a recently developed photoacoustic nanoprobe, based on silica-templated eumelanin-silver hybrid nanoparticles (MelaSil_Ag-NPs), with human serum albumin (HSA) is disclosed herein as an efficient and practical strategy to improve photostability and to perform SPARC mediated internalization in breast cancer cells. Modification of NPs with HSA induced a slight viability decrease in breast cancer cells (HS578T) and normal breast cells (MCF10a) when incubated with HSA-NPs up to 100 μg/mL concentration for 72 h and a complete suppression of hemotoxicity for long incubation times. Uptake experiments with MelaSil_Ag-HSA NPs indicated very high and selective internalization via SPARC in HS578T (SPARC positive cells) but not in MCF10a (SPARC negative cells), as evaluated by using endocytosis inhibitors. The binding of SPARC to HSA was confirmed by Co-IP and Dot-blot assays. Additional studies were performed to analyze the interaction of MelaSil_Ag-HSA NPs with protein corona. Data showed a dramatic diminution of interacting proteins in HSA conjugated NPs compared to bare NPs. HSA-coated MelaSil_Ag-NPs are thus disclosed as a novel functional nanohybrid for potential photoacoustic imaging applications.
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spelling pubmed-73608612020-07-29 Albumin-Modified Melanin-Silica Hybrid Nanoparticles Target Breast Cancer Cells via a SPARC-Dependent Mechanism Sanità, Gennaro Armanetti, Paolo Silvestri, Brigida Carrese, Barbara Calì, Gaetano Pota, Giulio Pezzella, Alessandro d’Ischia, Marco Luciani, Giuseppina Menichetti, Luca Lamberti, Annalisa Front Bioeng Biotechnol Bioengineering and Biotechnology Bioconjugation of a recently developed photoacoustic nanoprobe, based on silica-templated eumelanin-silver hybrid nanoparticles (MelaSil_Ag-NPs), with human serum albumin (HSA) is disclosed herein as an efficient and practical strategy to improve photostability and to perform SPARC mediated internalization in breast cancer cells. Modification of NPs with HSA induced a slight viability decrease in breast cancer cells (HS578T) and normal breast cells (MCF10a) when incubated with HSA-NPs up to 100 μg/mL concentration for 72 h and a complete suppression of hemotoxicity for long incubation times. Uptake experiments with MelaSil_Ag-HSA NPs indicated very high and selective internalization via SPARC in HS578T (SPARC positive cells) but not in MCF10a (SPARC negative cells), as evaluated by using endocytosis inhibitors. The binding of SPARC to HSA was confirmed by Co-IP and Dot-blot assays. Additional studies were performed to analyze the interaction of MelaSil_Ag-HSA NPs with protein corona. Data showed a dramatic diminution of interacting proteins in HSA conjugated NPs compared to bare NPs. HSA-coated MelaSil_Ag-NPs are thus disclosed as a novel functional nanohybrid for potential photoacoustic imaging applications. Frontiers Media S.A. 2020-07-08 /pmc/articles/PMC7360861/ /pubmed/32733871 http://dx.doi.org/10.3389/fbioe.2020.00765 Text en Copyright © 2020 Sanità, Armanetti, Silvestri, Carrese, Calì, Pota, Pezzella, d’Ischia, Luciani, Menichetti and Lamberti. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Sanità, Gennaro
Armanetti, Paolo
Silvestri, Brigida
Carrese, Barbara
Calì, Gaetano
Pota, Giulio
Pezzella, Alessandro
d’Ischia, Marco
Luciani, Giuseppina
Menichetti, Luca
Lamberti, Annalisa
Albumin-Modified Melanin-Silica Hybrid Nanoparticles Target Breast Cancer Cells via a SPARC-Dependent Mechanism
title Albumin-Modified Melanin-Silica Hybrid Nanoparticles Target Breast Cancer Cells via a SPARC-Dependent Mechanism
title_full Albumin-Modified Melanin-Silica Hybrid Nanoparticles Target Breast Cancer Cells via a SPARC-Dependent Mechanism
title_fullStr Albumin-Modified Melanin-Silica Hybrid Nanoparticles Target Breast Cancer Cells via a SPARC-Dependent Mechanism
title_full_unstemmed Albumin-Modified Melanin-Silica Hybrid Nanoparticles Target Breast Cancer Cells via a SPARC-Dependent Mechanism
title_short Albumin-Modified Melanin-Silica Hybrid Nanoparticles Target Breast Cancer Cells via a SPARC-Dependent Mechanism
title_sort albumin-modified melanin-silica hybrid nanoparticles target breast cancer cells via a sparc-dependent mechanism
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360861/
https://www.ncbi.nlm.nih.gov/pubmed/32733871
http://dx.doi.org/10.3389/fbioe.2020.00765
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