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Insulin Resistance in Apolipoprotein M Knockout Mice is Mediated by the Protein Kinase Akt Signaling Pathway
BACKGROUND: Previous clinical studies have suggested that apolipoprotein M (apoM) is involved in glucose metabolism and plays a causative role in insulin sensitivity. OBJECTIVE: The potential mechanism of apoM on modulating glucose homeostasis is explored and differentially expressed genes are anal...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bentham Science Publishers
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360917/ https://www.ncbi.nlm.nih.gov/pubmed/31702495 http://dx.doi.org/10.2174/1871530319666191023125820 |
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author | Yao, Shuang Zhang, Jun Zhan, Yuxia Shi, Yuanping Yu, Yang Zheng, Lu Xu, Ning Luo, Guanghua |
author_facet | Yao, Shuang Zhang, Jun Zhan, Yuxia Shi, Yuanping Yu, Yang Zheng, Lu Xu, Ning Luo, Guanghua |
author_sort | Yao, Shuang |
collection | PubMed |
description | BACKGROUND: Previous clinical studies have suggested that apolipoprotein M (apoM) is involved in glucose metabolism and plays a causative role in insulin sensitivity. OBJECTIVE: The potential mechanism of apoM on modulating glucose homeostasis is explored and
differentially expressed genes are analyzed by employing ApoM deficient (ApoM(-/-)) and wild type (WT) mice. METHODS: The metabolism of glucose in the hepatic tissues of high-fat diet ApoM(-/-) and WT mice was measured by a glycomics approach. Bioinformatic analysis was applied for analyzing the levels of differentially expressed mRNAs in the liver tissues of these mice. The insulin sensitivity of ApoM(-/-) and WT mice was compared using the insulin tolerance test and the phosphorylation levels of protein kinase Akt (AKT) and insulin stimulation in different tissues were examined by Western blot. RESULTS: The majority of the hepatic glucose metabolites exhibited lower concentration levels in the ApoM(-/-) mice compared with those of the WT mice. Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that ApoM deficiency affected the genes associated with the metabolism of glucose. The insulin tolerance test suggested that insulin sensitivity was impaired in ApoM(-/-) mice. The phosphorylation levels of AKT in muscle and adipose tissues of ApoM(-/-) mice were significantly diminished in response to insulin stimulation compared with those noted in WT mice. CONCLUSION: ApoM deficiency led to the disorders of glucose metabolism and altered genes related to glucose metabolism in mice liver. In vivo data indicated that apoM might augment insulin sensitivity by AKT-dependent mechanism. |
format | Online Article Text |
id | pubmed-7360917 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Bentham Science Publishers |
record_format | MEDLINE/PubMed |
spelling | pubmed-73609172020-07-30 Insulin Resistance in Apolipoprotein M Knockout Mice is Mediated by the Protein Kinase Akt Signaling Pathway Yao, Shuang Zhang, Jun Zhan, Yuxia Shi, Yuanping Yu, Yang Zheng, Lu Xu, Ning Luo, Guanghua Endocr Metab Immune Disord Drug Targets Article BACKGROUND: Previous clinical studies have suggested that apolipoprotein M (apoM) is involved in glucose metabolism and plays a causative role in insulin sensitivity. OBJECTIVE: The potential mechanism of apoM on modulating glucose homeostasis is explored and
differentially expressed genes are analyzed by employing ApoM deficient (ApoM(-/-)) and wild type (WT) mice. METHODS: The metabolism of glucose in the hepatic tissues of high-fat diet ApoM(-/-) and WT mice was measured by a glycomics approach. Bioinformatic analysis was applied for analyzing the levels of differentially expressed mRNAs in the liver tissues of these mice. The insulin sensitivity of ApoM(-/-) and WT mice was compared using the insulin tolerance test and the phosphorylation levels of protein kinase Akt (AKT) and insulin stimulation in different tissues were examined by Western blot. RESULTS: The majority of the hepatic glucose metabolites exhibited lower concentration levels in the ApoM(-/-) mice compared with those of the WT mice. Gene Ontology (GO) classification and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that ApoM deficiency affected the genes associated with the metabolism of glucose. The insulin tolerance test suggested that insulin sensitivity was impaired in ApoM(-/-) mice. The phosphorylation levels of AKT in muscle and adipose tissues of ApoM(-/-) mice were significantly diminished in response to insulin stimulation compared with those noted in WT mice. CONCLUSION: ApoM deficiency led to the disorders of glucose metabolism and altered genes related to glucose metabolism in mice liver. In vivo data indicated that apoM might augment insulin sensitivity by AKT-dependent mechanism. Bentham Science Publishers 2020-06 2020-06 /pmc/articles/PMC7360917/ /pubmed/31702495 http://dx.doi.org/10.2174/1871530319666191023125820 Text en © 2020 Bentham Science Publishers https://creativecommons.org/licenses/by-nc/4.0/legalcode This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited. |
spellingShingle | Article Yao, Shuang Zhang, Jun Zhan, Yuxia Shi, Yuanping Yu, Yang Zheng, Lu Xu, Ning Luo, Guanghua Insulin Resistance in Apolipoprotein M Knockout Mice is Mediated by the Protein Kinase Akt Signaling Pathway |
title | Insulin Resistance in Apolipoprotein M Knockout Mice is Mediated by the Protein Kinase Akt Signaling Pathway |
title_full | Insulin Resistance in Apolipoprotein M Knockout Mice is Mediated by the Protein Kinase Akt Signaling Pathway |
title_fullStr | Insulin Resistance in Apolipoprotein M Knockout Mice is Mediated by the Protein Kinase Akt Signaling Pathway |
title_full_unstemmed | Insulin Resistance in Apolipoprotein M Knockout Mice is Mediated by the Protein Kinase Akt Signaling Pathway |
title_short | Insulin Resistance in Apolipoprotein M Knockout Mice is Mediated by the Protein Kinase Akt Signaling Pathway |
title_sort | insulin resistance in apolipoprotein m knockout mice is mediated by the protein kinase akt signaling pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360917/ https://www.ncbi.nlm.nih.gov/pubmed/31702495 http://dx.doi.org/10.2174/1871530319666191023125820 |
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