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New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki

Larvae of the sleeping chironomid Polypedilum vanderplanki are known for their extraordinary ability to survive complete desiccation in an ametabolic state called “anhydrobiosis”. The unique feature of P. vanderplanki genome is the presence of expanded gene clusters associated with anhydrobiosis. Wh...

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Autores principales: Voronina, Taisiya A., Nesmelov, Alexander A., Kondratyeva, Sabina A., Deviatiiarov, Ruslan M., Miyata, Yugo, Tokumoto, Shoko, Cornette, Richard, Gusev, Oleg A., Kikawada, Takahiro, Shagimardanova, Elena I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7363813/
https://www.ncbi.nlm.nih.gov/pubmed/32669703
http://dx.doi.org/10.1038/s41598-020-68330-6
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author Voronina, Taisiya A.
Nesmelov, Alexander A.
Kondratyeva, Sabina A.
Deviatiiarov, Ruslan M.
Miyata, Yugo
Tokumoto, Shoko
Cornette, Richard
Gusev, Oleg A.
Kikawada, Takahiro
Shagimardanova, Elena I.
author_facet Voronina, Taisiya A.
Nesmelov, Alexander A.
Kondratyeva, Sabina A.
Deviatiiarov, Ruslan M.
Miyata, Yugo
Tokumoto, Shoko
Cornette, Richard
Gusev, Oleg A.
Kikawada, Takahiro
Shagimardanova, Elena I.
author_sort Voronina, Taisiya A.
collection PubMed
description Larvae of the sleeping chironomid Polypedilum vanderplanki are known for their extraordinary ability to survive complete desiccation in an ametabolic state called “anhydrobiosis”. The unique feature of P. vanderplanki genome is the presence of expanded gene clusters associated with anhydrobiosis. While several such clusters represent orthologues of known genes, there is a distinct set of genes unique for P. vanderplanki. These include Lea-Island-Located (LIL) genes with no known orthologues except two of LEA genes of P. vanderplanki, PvLea1 and PvLea3. However, PvLIL proteins lack typical features of LEA such as the state of intrinsic disorder, hydrophilicity and characteristic LEA_4 motif. They possess four to five transmembrane domains each and we confirmed membrane targeting for three PvLILs. Conserved amino acids in PvLIL are located in transmembrane domains or nearby. PvLEA1 and PvLEA3 proteins are chimeras combining LEA-like parts and transmembrane domains, shared with PvLIL proteins. We have found that PvLil genes are highly upregulated during anhydrobiosis induction both in larvae of P. vanderplanki and P. vanderplanki-derived cultured cell line, Pv11. Thus, PvLil are a new intriguing group of genes that are likely to be associated with anhydrobiosis due to their common origin with some LEA genes and their induction during anhydrobiosis.
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spelling pubmed-73638132020-07-16 New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki Voronina, Taisiya A. Nesmelov, Alexander A. Kondratyeva, Sabina A. Deviatiiarov, Ruslan M. Miyata, Yugo Tokumoto, Shoko Cornette, Richard Gusev, Oleg A. Kikawada, Takahiro Shagimardanova, Elena I. Sci Rep Article Larvae of the sleeping chironomid Polypedilum vanderplanki are known for their extraordinary ability to survive complete desiccation in an ametabolic state called “anhydrobiosis”. The unique feature of P. vanderplanki genome is the presence of expanded gene clusters associated with anhydrobiosis. While several such clusters represent orthologues of known genes, there is a distinct set of genes unique for P. vanderplanki. These include Lea-Island-Located (LIL) genes with no known orthologues except two of LEA genes of P. vanderplanki, PvLea1 and PvLea3. However, PvLIL proteins lack typical features of LEA such as the state of intrinsic disorder, hydrophilicity and characteristic LEA_4 motif. They possess four to five transmembrane domains each and we confirmed membrane targeting for three PvLILs. Conserved amino acids in PvLIL are located in transmembrane domains or nearby. PvLEA1 and PvLEA3 proteins are chimeras combining LEA-like parts and transmembrane domains, shared with PvLIL proteins. We have found that PvLil genes are highly upregulated during anhydrobiosis induction both in larvae of P. vanderplanki and P. vanderplanki-derived cultured cell line, Pv11. Thus, PvLil are a new intriguing group of genes that are likely to be associated with anhydrobiosis due to their common origin with some LEA genes and their induction during anhydrobiosis. Nature Publishing Group UK 2020-07-15 /pmc/articles/PMC7363813/ /pubmed/32669703 http://dx.doi.org/10.1038/s41598-020-68330-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Voronina, Taisiya A.
Nesmelov, Alexander A.
Kondratyeva, Sabina A.
Deviatiiarov, Ruslan M.
Miyata, Yugo
Tokumoto, Shoko
Cornette, Richard
Gusev, Oleg A.
Kikawada, Takahiro
Shagimardanova, Elena I.
New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
title New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
title_full New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
title_fullStr New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
title_full_unstemmed New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
title_short New group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge Polypedilum vanderplanki
title_sort new group of transmembrane proteins associated with desiccation tolerance in the anhydrobiotic midge polypedilum vanderplanki
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7363813/
https://www.ncbi.nlm.nih.gov/pubmed/32669703
http://dx.doi.org/10.1038/s41598-020-68330-6
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