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Identification and Fine Mapping of RppM, a Southern Corn Rust Resistance Gene in Maize

Southern corn rust (SCR) caused by Puccinia polysora Underw. is a major disease causing severe yield losses during maize production. Here, we identified and mapped the SCR resistance gene RppM from the near-isogenic line Kangxiujing2416 (Jing2416K), which harbors RppM in the genetic background of th...

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Autores principales: Wang, Shuai, Zhang, Ruyang, Shi, Zi, Zhao, Yanxin, Su, Aiguo, Wang, Yuandong, Xing, Jinfeng, Ge, Jianrong, Li, Chunhui, Wang, Xiaqing, Wang, Jidong, Sun, Xuan, Liu, Qian, Chen, Yining, Zhang, Yunxia, Wang, Shuaishuai, Song, Wei, Zhao, Jiuran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7363983/
https://www.ncbi.nlm.nih.gov/pubmed/32733529
http://dx.doi.org/10.3389/fpls.2020.01057
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author Wang, Shuai
Zhang, Ruyang
Shi, Zi
Zhao, Yanxin
Su, Aiguo
Wang, Yuandong
Xing, Jinfeng
Ge, Jianrong
Li, Chunhui
Wang, Xiaqing
Wang, Jidong
Sun, Xuan
Liu, Qian
Chen, Yining
Zhang, Yunxia
Wang, Shuaishuai
Song, Wei
Zhao, Jiuran
author_facet Wang, Shuai
Zhang, Ruyang
Shi, Zi
Zhao, Yanxin
Su, Aiguo
Wang, Yuandong
Xing, Jinfeng
Ge, Jianrong
Li, Chunhui
Wang, Xiaqing
Wang, Jidong
Sun, Xuan
Liu, Qian
Chen, Yining
Zhang, Yunxia
Wang, Shuaishuai
Song, Wei
Zhao, Jiuran
author_sort Wang, Shuai
collection PubMed
description Southern corn rust (SCR) caused by Puccinia polysora Underw. is a major disease causing severe yield losses during maize production. Here, we identified and mapped the SCR resistance gene RppM from the near-isogenic line Kangxiujing2416 (Jing2416K), which harbors RppM in the genetic background of the susceptible inbred line Jing2416. In this study, the inheritance of SCR resistance was investigated in F(2) and F(3) populations derived from a cross between Jing2416K and Jing2416. The observed 3:1 segregation ratio of resistant to susceptible plants indicated that the SCR resistance is controlled by a single dominant gene. Using an F(2) population, we performed bulked segregant analysis (BSA) sequencing and mapped RppM to a 3.69-Mb region on chromosome arm 10S. To further narrow down the region harboring RppM, we developed 13 insertion/deletion (InDel) markers based on the sequencing data. Finally, RppM was mapped to a region spanning 110-kb using susceptible individuals from a large F(2) population. Two genes (Zm00001d023265 and Zm00001d023267) encoding putative CC-NBS-LRR (coiled-coiled, nucleotide-binding site, and leucine-rich repeat) proteins, a common characteristic of R genes, were located in this region (B73 RefGen_v4 reference genome). Sequencing and comparison of the two genes cloned from Jing2416K and Jing2416 revealed sequence variations in their coding regions. The relative expression levels of these two genes in Jing2416K were found to be significantly higher than those in Jing2416. Zm00001d023265 and Zm00001d023267 are thus potential RppM candidates.
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spelling pubmed-73639832020-07-29 Identification and Fine Mapping of RppM, a Southern Corn Rust Resistance Gene in Maize Wang, Shuai Zhang, Ruyang Shi, Zi Zhao, Yanxin Su, Aiguo Wang, Yuandong Xing, Jinfeng Ge, Jianrong Li, Chunhui Wang, Xiaqing Wang, Jidong Sun, Xuan Liu, Qian Chen, Yining Zhang, Yunxia Wang, Shuaishuai Song, Wei Zhao, Jiuran Front Plant Sci Plant Science Southern corn rust (SCR) caused by Puccinia polysora Underw. is a major disease causing severe yield losses during maize production. Here, we identified and mapped the SCR resistance gene RppM from the near-isogenic line Kangxiujing2416 (Jing2416K), which harbors RppM in the genetic background of the susceptible inbred line Jing2416. In this study, the inheritance of SCR resistance was investigated in F(2) and F(3) populations derived from a cross between Jing2416K and Jing2416. The observed 3:1 segregation ratio of resistant to susceptible plants indicated that the SCR resistance is controlled by a single dominant gene. Using an F(2) population, we performed bulked segregant analysis (BSA) sequencing and mapped RppM to a 3.69-Mb region on chromosome arm 10S. To further narrow down the region harboring RppM, we developed 13 insertion/deletion (InDel) markers based on the sequencing data. Finally, RppM was mapped to a region spanning 110-kb using susceptible individuals from a large F(2) population. Two genes (Zm00001d023265 and Zm00001d023267) encoding putative CC-NBS-LRR (coiled-coiled, nucleotide-binding site, and leucine-rich repeat) proteins, a common characteristic of R genes, were located in this region (B73 RefGen_v4 reference genome). Sequencing and comparison of the two genes cloned from Jing2416K and Jing2416 revealed sequence variations in their coding regions. The relative expression levels of these two genes in Jing2416K were found to be significantly higher than those in Jing2416. Zm00001d023265 and Zm00001d023267 are thus potential RppM candidates. Frontiers Media S.A. 2020-07-09 /pmc/articles/PMC7363983/ /pubmed/32733529 http://dx.doi.org/10.3389/fpls.2020.01057 Text en Copyright © 2020 Wang, Zhang, Shi, Zhao, Su, Wang, Xing, Ge, Li, Wang, Wang, Sun, Liu, Chen, Zhang, Wang, Song and Zhao http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Shuai
Zhang, Ruyang
Shi, Zi
Zhao, Yanxin
Su, Aiguo
Wang, Yuandong
Xing, Jinfeng
Ge, Jianrong
Li, Chunhui
Wang, Xiaqing
Wang, Jidong
Sun, Xuan
Liu, Qian
Chen, Yining
Zhang, Yunxia
Wang, Shuaishuai
Song, Wei
Zhao, Jiuran
Identification and Fine Mapping of RppM, a Southern Corn Rust Resistance Gene in Maize
title Identification and Fine Mapping of RppM, a Southern Corn Rust Resistance Gene in Maize
title_full Identification and Fine Mapping of RppM, a Southern Corn Rust Resistance Gene in Maize
title_fullStr Identification and Fine Mapping of RppM, a Southern Corn Rust Resistance Gene in Maize
title_full_unstemmed Identification and Fine Mapping of RppM, a Southern Corn Rust Resistance Gene in Maize
title_short Identification and Fine Mapping of RppM, a Southern Corn Rust Resistance Gene in Maize
title_sort identification and fine mapping of rppm, a southern corn rust resistance gene in maize
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7363983/
https://www.ncbi.nlm.nih.gov/pubmed/32733529
http://dx.doi.org/10.3389/fpls.2020.01057
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