Cargando…
Integrated metabolomic and transcriptomic profiling reveals the tissue-specific flavonoid compositions and their biosynthesis pathways in Ziziphora bungeana
BACKGROUND: Ziziphora bungeana Juz. is a folk medicine from the Xinjiang Uygur Autonomous Region. The herb or the aerial parts of it have been used to medicinally treat cardiovascular diseases. Flavonoids are the main pharmacologically active ingredients in Z. bungeana. Identification of the tissue-...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7364582/ https://www.ncbi.nlm.nih.gov/pubmed/32695217 http://dx.doi.org/10.1186/s13020-020-00354-6 |
_version_ | 1783559858865307648 |
---|---|
author | He, Jiang Yang, Weijun Cheng, Bo Ma, Lina Tursunjiang, Dilinuer Ding, Zimian Li, Yong Wang, Zhaofeng Ma, Yimian Li, Guan |
author_facet | He, Jiang Yang, Weijun Cheng, Bo Ma, Lina Tursunjiang, Dilinuer Ding, Zimian Li, Yong Wang, Zhaofeng Ma, Yimian Li, Guan |
author_sort | He, Jiang |
collection | PubMed |
description | BACKGROUND: Ziziphora bungeana Juz. is a folk medicine from the Xinjiang Uygur Autonomous Region. The herb or the aerial parts of it have been used to medicinally treat cardiovascular diseases. Flavonoids are the main pharmacologically active ingredients in Z. bungeana. Identification of the tissue-specific distribution of flavonoids in Z. bungeana is crucial for effective and sustainable medicinal use of the plant. Furthermore, understanding of the biosynthesis pathways of these flavonoids in Z. bungeana is of great biological significance. METHODS: The flavonoids from different tissues of Z. bungeana were identified using liquid chromatography-tandem mass spectrometry (LC–MS/MS). The full-length transcriptome of Z. bungeana was determined using a strategy based on a combination of Illumina and PacBio sequencing techniques. The functions of differentially expressed unigenes were predicted using bioinformatics methods and further investigated by real-time quantitative PCR and phylogenetic relationship analysis. RESULTS: Among the 12 major flavonoid components identified from Z. bungeana extracts, linarin was the most abundant component. Nine flavonoids were identified as characteristic components of specific tissues. Transcriptome profiling and bioinformatic analysis revealed that 18 genes were putatively involved in flavonoid biosynthesis. The gene expression and phylogenetic analysis results indicated that ZbPALs, Zb4CL3, ZbCHS1, and ZbCHI1 may be involved in the biosynthesis of the main flavonoid intermediate. ZbFNSII, ZbANS, and ZbFLS may be involved in the biosynthesis of flavones, anthocyanins, and flavonols, respectively. A map of the biosynthesis pathways of the 12 major flavonoids in Z. bungeana is proposed. CONCLUSIONS: The chemical constituent analysis revealed the compositions of 9 characteristic flavonoids in different tissues of Z. bungeana. Linarin can be hydrolysed into acacetin to exert a pharmaceutical role. Apigenin-7-O-rutinoside is hypothesised to be the precursor of linarin in Z. bungeana. There was greater content of linarin in the aerial parts of the plant than in the whole herb, which provides a theoretical basis for using the aerial parts of Z. bungeana for medicine. These results provide a valuable reference for further research on the flavonoid biosynthesis pathways of Z. bungeana and will be significant for the effective utilisation and ecological protection of Z. bungeana. |
format | Online Article Text |
id | pubmed-7364582 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-73645822020-07-20 Integrated metabolomic and transcriptomic profiling reveals the tissue-specific flavonoid compositions and their biosynthesis pathways in Ziziphora bungeana He, Jiang Yang, Weijun Cheng, Bo Ma, Lina Tursunjiang, Dilinuer Ding, Zimian Li, Yong Wang, Zhaofeng Ma, Yimian Li, Guan Chin Med Research BACKGROUND: Ziziphora bungeana Juz. is a folk medicine from the Xinjiang Uygur Autonomous Region. The herb or the aerial parts of it have been used to medicinally treat cardiovascular diseases. Flavonoids are the main pharmacologically active ingredients in Z. bungeana. Identification of the tissue-specific distribution of flavonoids in Z. bungeana is crucial for effective and sustainable medicinal use of the plant. Furthermore, understanding of the biosynthesis pathways of these flavonoids in Z. bungeana is of great biological significance. METHODS: The flavonoids from different tissues of Z. bungeana were identified using liquid chromatography-tandem mass spectrometry (LC–MS/MS). The full-length transcriptome of Z. bungeana was determined using a strategy based on a combination of Illumina and PacBio sequencing techniques. The functions of differentially expressed unigenes were predicted using bioinformatics methods and further investigated by real-time quantitative PCR and phylogenetic relationship analysis. RESULTS: Among the 12 major flavonoid components identified from Z. bungeana extracts, linarin was the most abundant component. Nine flavonoids were identified as characteristic components of specific tissues. Transcriptome profiling and bioinformatic analysis revealed that 18 genes were putatively involved in flavonoid biosynthesis. The gene expression and phylogenetic analysis results indicated that ZbPALs, Zb4CL3, ZbCHS1, and ZbCHI1 may be involved in the biosynthesis of the main flavonoid intermediate. ZbFNSII, ZbANS, and ZbFLS may be involved in the biosynthesis of flavones, anthocyanins, and flavonols, respectively. A map of the biosynthesis pathways of the 12 major flavonoids in Z. bungeana is proposed. CONCLUSIONS: The chemical constituent analysis revealed the compositions of 9 characteristic flavonoids in different tissues of Z. bungeana. Linarin can be hydrolysed into acacetin to exert a pharmaceutical role. Apigenin-7-O-rutinoside is hypothesised to be the precursor of linarin in Z. bungeana. There was greater content of linarin in the aerial parts of the plant than in the whole herb, which provides a theoretical basis for using the aerial parts of Z. bungeana for medicine. These results provide a valuable reference for further research on the flavonoid biosynthesis pathways of Z. bungeana and will be significant for the effective utilisation and ecological protection of Z. bungeana. BioMed Central 2020-07-16 /pmc/articles/PMC7364582/ /pubmed/32695217 http://dx.doi.org/10.1186/s13020-020-00354-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research He, Jiang Yang, Weijun Cheng, Bo Ma, Lina Tursunjiang, Dilinuer Ding, Zimian Li, Yong Wang, Zhaofeng Ma, Yimian Li, Guan Integrated metabolomic and transcriptomic profiling reveals the tissue-specific flavonoid compositions and their biosynthesis pathways in Ziziphora bungeana |
title | Integrated metabolomic and transcriptomic profiling reveals the tissue-specific flavonoid compositions and their biosynthesis pathways in Ziziphora bungeana |
title_full | Integrated metabolomic and transcriptomic profiling reveals the tissue-specific flavonoid compositions and their biosynthesis pathways in Ziziphora bungeana |
title_fullStr | Integrated metabolomic and transcriptomic profiling reveals the tissue-specific flavonoid compositions and their biosynthesis pathways in Ziziphora bungeana |
title_full_unstemmed | Integrated metabolomic and transcriptomic profiling reveals the tissue-specific flavonoid compositions and their biosynthesis pathways in Ziziphora bungeana |
title_short | Integrated metabolomic and transcriptomic profiling reveals the tissue-specific flavonoid compositions and their biosynthesis pathways in Ziziphora bungeana |
title_sort | integrated metabolomic and transcriptomic profiling reveals the tissue-specific flavonoid compositions and their biosynthesis pathways in ziziphora bungeana |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7364582/ https://www.ncbi.nlm.nih.gov/pubmed/32695217 http://dx.doi.org/10.1186/s13020-020-00354-6 |
work_keys_str_mv | AT hejiang integratedmetabolomicandtranscriptomicprofilingrevealsthetissuespecificflavonoidcompositionsandtheirbiosynthesispathwaysinziziphorabungeana AT yangweijun integratedmetabolomicandtranscriptomicprofilingrevealsthetissuespecificflavonoidcompositionsandtheirbiosynthesispathwaysinziziphorabungeana AT chengbo integratedmetabolomicandtranscriptomicprofilingrevealsthetissuespecificflavonoidcompositionsandtheirbiosynthesispathwaysinziziphorabungeana AT malina integratedmetabolomicandtranscriptomicprofilingrevealsthetissuespecificflavonoidcompositionsandtheirbiosynthesispathwaysinziziphorabungeana AT tursunjiangdilinuer integratedmetabolomicandtranscriptomicprofilingrevealsthetissuespecificflavonoidcompositionsandtheirbiosynthesispathwaysinziziphorabungeana AT dingzimian integratedmetabolomicandtranscriptomicprofilingrevealsthetissuespecificflavonoidcompositionsandtheirbiosynthesispathwaysinziziphorabungeana AT liyong integratedmetabolomicandtranscriptomicprofilingrevealsthetissuespecificflavonoidcompositionsandtheirbiosynthesispathwaysinziziphorabungeana AT wangzhaofeng integratedmetabolomicandtranscriptomicprofilingrevealsthetissuespecificflavonoidcompositionsandtheirbiosynthesispathwaysinziziphorabungeana AT mayimian integratedmetabolomicandtranscriptomicprofilingrevealsthetissuespecificflavonoidcompositionsandtheirbiosynthesispathwaysinziziphorabungeana AT liguan integratedmetabolomicandtranscriptomicprofilingrevealsthetissuespecificflavonoidcompositionsandtheirbiosynthesispathwaysinziziphorabungeana |