Cargando…

微RNA-181a在核型正常急性髓系白血病患者中的表达及临床意义

OBJECTIVE: To study the expression of miRNA-181a in acute myeloid leukemia (AML) patients with normal karyotype to probe its prognosis significance. METHODS: The expression level of miRNA-181a in bone marrow mononuclear cells of 120 de novo AML patients with normal karyotype was detected by real tim...

Descripción completa

Detalles Bibliográficos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Editorial office of Chinese Journal of Hematology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7364963/
https://www.ncbi.nlm.nih.gov/pubmed/29166738
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2017.10.007
_version_ 1783559948618170368
collection PubMed
description OBJECTIVE: To study the expression of miRNA-181a in acute myeloid leukemia (AML) patients with normal karyotype to probe its prognosis significance. METHODS: The expression level of miRNA-181a in bone marrow mononuclear cells of 120 de novo AML patients with normal karyotype was detected by real time fluorescence quantitative PCR. The direct sequencing method was used to detect IDH1, IDH2, NPM1, FLT3-ITD, DNMT3A and CEBPα mutations in CN-AML patients after PCR. The relationship between miRNA-181a expression and gene mutation, the clinical parameters and prognosis were analyzed. RESULTS: The rates of overall surviva1 (OS) in high expression and low expression groups were 25.0 months and 15.0 months, respectively (P<0.05). Relapse free survival (RFS) in high expression and low expression groups were 21.4 months and 11.2 months, respectively (P<0.05). Significantly higher level hemoglobin, complete remission rate and proportion of wild type NPM1 expression in the high expression of miRNA-181a group were observed when compared with the lower expression of miRNA-181a group (P<0.05). Multivariate Cox regression analysis showed miRNA-181a overexpression was an independent prognostic factor for CN-AML (HR=2.219, 95%CI 1.601~2.432, P=0.018). CONCLUSION: Higher expression of miRNA-181a was a good prognostic factor independent of clinical parameters and high frequency gene mutations, which implicated that the miRNA-181a expression level could be used as an important prognostic indicator of AML patients with normal karyotype.
format Online
Article
Text
id pubmed-7364963
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Editorial office of Chinese Journal of Hematology
record_format MEDLINE/PubMed
spelling pubmed-73649632020-07-16 微RNA-181a在核型正常急性髓系白血病患者中的表达及临床意义 Zhonghua Xue Ye Xue Za Zhi 论著 OBJECTIVE: To study the expression of miRNA-181a in acute myeloid leukemia (AML) patients with normal karyotype to probe its prognosis significance. METHODS: The expression level of miRNA-181a in bone marrow mononuclear cells of 120 de novo AML patients with normal karyotype was detected by real time fluorescence quantitative PCR. The direct sequencing method was used to detect IDH1, IDH2, NPM1, FLT3-ITD, DNMT3A and CEBPα mutations in CN-AML patients after PCR. The relationship between miRNA-181a expression and gene mutation, the clinical parameters and prognosis were analyzed. RESULTS: The rates of overall surviva1 (OS) in high expression and low expression groups were 25.0 months and 15.0 months, respectively (P<0.05). Relapse free survival (RFS) in high expression and low expression groups were 21.4 months and 11.2 months, respectively (P<0.05). Significantly higher level hemoglobin, complete remission rate and proportion of wild type NPM1 expression in the high expression of miRNA-181a group were observed when compared with the lower expression of miRNA-181a group (P<0.05). Multivariate Cox regression analysis showed miRNA-181a overexpression was an independent prognostic factor for CN-AML (HR=2.219, 95%CI 1.601~2.432, P=0.018). CONCLUSION: Higher expression of miRNA-181a was a good prognostic factor independent of clinical parameters and high frequency gene mutations, which implicated that the miRNA-181a expression level could be used as an important prognostic indicator of AML patients with normal karyotype. Editorial office of Chinese Journal of Hematology 2017-10 /pmc/articles/PMC7364963/ /pubmed/29166738 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2017.10.007 Text en 2017年版权归中华医学会所有 http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License (CC-BY-NC). The Copyright own by Publisher. Without authorization, shall not reprint, except this publication article, shall not use this publication format design. Unless otherwise stated, all articles published in this journal do not represent the views of the Chinese Medical Association or the editorial board of this journal.
spellingShingle 论著
微RNA-181a在核型正常急性髓系白血病患者中的表达及临床意义
title 微RNA-181a在核型正常急性髓系白血病患者中的表达及临床意义
title_full 微RNA-181a在核型正常急性髓系白血病患者中的表达及临床意义
title_fullStr 微RNA-181a在核型正常急性髓系白血病患者中的表达及临床意义
title_full_unstemmed 微RNA-181a在核型正常急性髓系白血病患者中的表达及临床意义
title_short 微RNA-181a在核型正常急性髓系白血病患者中的表达及临床意义
title_sort 微rna-181a在核型正常急性髓系白血病患者中的表达及临床意义
topic 论著
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7364963/
https://www.ncbi.nlm.nih.gov/pubmed/29166738
http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2017.10.007
work_keys_str_mv AT wēirna181azàihéxíngzhèngchángjíxìngsuǐxìbáixuèbìnghuànzhězhōngdebiǎodájílínchuángyìyì
AT wēirna181azàihéxíngzhèngchángjíxìngsuǐxìbáixuèbìnghuànzhězhōngdebiǎodájílínchuángyìyì
AT wēirna181azàihéxíngzhèngchángjíxìngsuǐxìbáixuèbìnghuànzhězhōngdebiǎodájílínchuángyìyì
AT wēirna181azàihéxíngzhèngchángjíxìngsuǐxìbáixuèbìnghuànzhězhōngdebiǎodájílínchuángyìyì
AT wēirna181azàihéxíngzhèngchángjíxìngsuǐxìbáixuèbìnghuànzhězhōngdebiǎodájílínchuángyìyì
AT wēirna181azàihéxíngzhèngchángjíxìngsuǐxìbáixuèbìnghuànzhězhōngdebiǎodájílínchuángyìyì
AT wēirna181azàihéxíngzhèngchángjíxìngsuǐxìbáixuèbìnghuànzhězhōngdebiǎodájílínchuángyìyì
AT wēirna181azàihéxíngzhèngchángjíxìngsuǐxìbáixuèbìnghuànzhězhōngdebiǎodájílínchuángyìyì
AT wēirna181azàihéxíngzhèngchángjíxìngsuǐxìbáixuèbìnghuànzhězhōngdebiǎodájílínchuángyìyì
AT wēirna181azàihéxíngzhèngchángjíxìngsuǐxìbáixuèbìnghuànzhězhōngdebiǎodájílínchuángyìyì