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初诊t(8;21)急性髓系白血病中白血病干细胞相关抗原的表达特征及预后意义
OBJECTIVE: To investigate the characteristic and prognostic significance of leukemia stem cells associated antigens expressions including CD34, CD38, CD123, CD96 and TIM-3 in t(8;21) AML. METHODS: Bone marrow samples of 47 t(8;21) AML patients were collected at diagnosis from October 2015 to April 2...
Formato: | Online Artículo Texto |
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Lenguaje: | English |
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Editorial office of Chinese Journal of Hematology
2019
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7364990/ https://www.ncbi.nlm.nih.gov/pubmed/31775482 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2019.10.007 |
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collection | PubMed |
description | OBJECTIVE: To investigate the characteristic and prognostic significance of leukemia stem cells associated antigens expressions including CD34, CD38, CD123, CD96 and TIM-3 in t(8;21) AML. METHODS: Bone marrow samples of 47 t(8;21) AML patients were collected at diagnosis from October 2015 to April 2018 in Peking University Peoples' Hospital, then flow cytometry method was performed to detect the expression frequencies of CD34, CD38, CD123, CD96 and TIM-3 to analyze the relationship between leukemia stem cells associated antigens expressions and relapse. RESULTS: Of 47 t(8;21) AML patients tested, the median percentages of CD34(+)CD38(−), CD34(+) CD38(−)CD123(+), CD34(+)CD38(−) CD96(+) and CD34(+) CD38(−) TIM-3(+) cells among nucleated cells were 2.37%, 0.24%, 0.27% and 0.06%, respectively. All the frequencies of CD34(+)CD38(−), CD34(+)CD38(−)CD123(+), CD34(+)CD38(−)CD96(+) and CD34(+) CD38(−)TIM-3(+)cells had no impact on the achievement of CR after the first course of induction. All higher frequencies of CD34(+)CD38(−), CD34(+)CD38(−)CD123(+), CD34(+)CD38(−)CD96(+) cells were related to higher 2-year CIR rate. Whereas, the frequency of CD34(+) CD38(−) TIM-3(+) cells had no impact on CIR rate. Both high frequency of CD34(+) CD38(−) cells and the high level of minimal residual diseases (patients with <3-log reduction in the RUNX1-RUNX1T1 transcript level after the second consolidation therapy) were independent poor prognostic factors of CIR[P=0.025, HR=6.9 (95%CI1.3–37.4) ; P=0.031, HR=11.1 (95%CI 1.2–99.2) ]. CONCLUSION: Different leukemia stem cells associated antigens had distinct prognostic significance in t(8;21) AML. High frequencies of CD34(+) CD38(−), CD34(+) CD38(−) CD123(+) and CD34(+)CD38(−)CD96(+) cells at diagnosis predicted relapse in patients with t(8;21) AML. |
format | Online Article Text |
id | pubmed-7364990 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Editorial office of Chinese Journal of Hematology |
record_format | MEDLINE/PubMed |
spelling | pubmed-73649902020-07-16 初诊t(8;21)急性髓系白血病中白血病干细胞相关抗原的表达特征及预后意义 Zhonghua Xue Ye Xue Za Zhi 论著 OBJECTIVE: To investigate the characteristic and prognostic significance of leukemia stem cells associated antigens expressions including CD34, CD38, CD123, CD96 and TIM-3 in t(8;21) AML. METHODS: Bone marrow samples of 47 t(8;21) AML patients were collected at diagnosis from October 2015 to April 2018 in Peking University Peoples' Hospital, then flow cytometry method was performed to detect the expression frequencies of CD34, CD38, CD123, CD96 and TIM-3 to analyze the relationship between leukemia stem cells associated antigens expressions and relapse. RESULTS: Of 47 t(8;21) AML patients tested, the median percentages of CD34(+)CD38(−), CD34(+) CD38(−)CD123(+), CD34(+)CD38(−) CD96(+) and CD34(+) CD38(−) TIM-3(+) cells among nucleated cells were 2.37%, 0.24%, 0.27% and 0.06%, respectively. All the frequencies of CD34(+)CD38(−), CD34(+)CD38(−)CD123(+), CD34(+)CD38(−)CD96(+) and CD34(+) CD38(−)TIM-3(+)cells had no impact on the achievement of CR after the first course of induction. All higher frequencies of CD34(+)CD38(−), CD34(+)CD38(−)CD123(+), CD34(+)CD38(−)CD96(+) cells were related to higher 2-year CIR rate. Whereas, the frequency of CD34(+) CD38(−) TIM-3(+) cells had no impact on CIR rate. Both high frequency of CD34(+) CD38(−) cells and the high level of minimal residual diseases (patients with <3-log reduction in the RUNX1-RUNX1T1 transcript level after the second consolidation therapy) were independent poor prognostic factors of CIR[P=0.025, HR=6.9 (95%CI1.3–37.4) ; P=0.031, HR=11.1 (95%CI 1.2–99.2) ]. CONCLUSION: Different leukemia stem cells associated antigens had distinct prognostic significance in t(8;21) AML. High frequencies of CD34(+) CD38(−), CD34(+) CD38(−) CD123(+) and CD34(+)CD38(−)CD96(+) cells at diagnosis predicted relapse in patients with t(8;21) AML. Editorial office of Chinese Journal of Hematology 2019-10 /pmc/articles/PMC7364990/ /pubmed/31775482 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2019.10.007 Text en 2019年版权归中华医学会所有 http://creativecommons.org/licenses/by-nc-sa/3.0/ This work is licensed under a Creative Commons Attribution 3.0 License (CC-BY-NC). The Copyright own by Publisher. Without authorization, shall not reprint, except this publication article, shall not use this publication format design. Unless otherwise stated, all articles published in this journal do not represent the views of the Chinese Medical Association or the editorial board of this journal. |
spellingShingle | 论著 初诊t(8;21)急性髓系白血病中白血病干细胞相关抗原的表达特征及预后意义 |
title | 初诊t(8;21)急性髓系白血病中白血病干细胞相关抗原的表达特征及预后意义 |
title_full | 初诊t(8;21)急性髓系白血病中白血病干细胞相关抗原的表达特征及预后意义 |
title_fullStr | 初诊t(8;21)急性髓系白血病中白血病干细胞相关抗原的表达特征及预后意义 |
title_full_unstemmed | 初诊t(8;21)急性髓系白血病中白血病干细胞相关抗原的表达特征及预后意义 |
title_short | 初诊t(8;21)急性髓系白血病中白血病干细胞相关抗原的表达特征及预后意义 |
title_sort | 初诊t(8;21)急性髓系白血病中白血病干细胞相关抗原的表达特征及预后意义 |
topic | 论著 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7364990/ https://www.ncbi.nlm.nih.gov/pubmed/31775482 http://dx.doi.org/10.3760/cma.j.issn.0253-2727.2019.10.007 |
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