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Differential Expression of Heat Shock Protein 27 in Oral Epithelial Dysplasias and Squamous Cell Carcinoma
BACKGROUND: Oral squamous cell carcinoma (OSCC) is the most devastating neoplasm with dramatic increase in morbidity and mortality. The detection and prognostic evaluation of precancerous lesions could aid in early control of cancer. Heat shock protein (HSP) 27 has found to be a biomarker and therap...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365515/ https://www.ncbi.nlm.nih.gov/pubmed/32766120 http://dx.doi.org/10.4103/JMAU.JMAU_48_19 |
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author | Karri, Roja Lakshmi Subramanyam, R. Venkata Venigella, Aparna Babburi, Suresh Pinisetti, Soujanya Rudraraju, Amrutha |
author_facet | Karri, Roja Lakshmi Subramanyam, R. Venkata Venigella, Aparna Babburi, Suresh Pinisetti, Soujanya Rudraraju, Amrutha |
author_sort | Karri, Roja Lakshmi |
collection | PubMed |
description | BACKGROUND: Oral squamous cell carcinoma (OSCC) is the most devastating neoplasm with dramatic increase in morbidity and mortality. The detection and prognostic evaluation of precancerous lesions could aid in early control of cancer. Heat shock protein (HSP) 27 has found to be a biomarker and therapeutic target in different types of cancer. AIM: This study aims to investigate the role of HSP 27 as prognostic molecular indicator of malignant transformation in oral epithelial dysplasias. MATERIALS AND METHODS: Thirty samples of epithelial dysplasia (10 mild dysplasia, 10 moderate dysplasia, and 10 severe dysplasia/carcinoma in situ cases), 10 samples each of well-differentiated OSCC and normal oral mucosa were routinely processed, formalin-fixed, paraffin-embedded, and analyzed for HSP27 expression by immunohistochemistry. Statistical analysis was done by one way-ANOVA and Mann–Whitney test to assess the differences between two individual groups. RESULTS: Normal mucosa showed intense, but nonuniform, expression of HSP27. An initial decline was noted in dysplasias. A significant correlation of HSP27 expression was observed with the severity of dysplasia and well-differentiated OSCC (P < 0.05). CONCLUSION: Low HSP 27 expression can be considered as early molecular indicator of initial dysplastic change in normal mucosa. An overexpression of HSP 27 in clinically and histologically confirmed dysplasia could indicate likely transformation to well-differentiated OSCC and could be of prognostic value. However, further studies with a larger sample size are required to confirm the role of HSP 27 as predictive indicator. |
format | Online Article Text |
id | pubmed-7365515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-73655152020-08-05 Differential Expression of Heat Shock Protein 27 in Oral Epithelial Dysplasias and Squamous Cell Carcinoma Karri, Roja Lakshmi Subramanyam, R. Venkata Venigella, Aparna Babburi, Suresh Pinisetti, Soujanya Rudraraju, Amrutha J Microsc Ultrastruct Original Article BACKGROUND: Oral squamous cell carcinoma (OSCC) is the most devastating neoplasm with dramatic increase in morbidity and mortality. The detection and prognostic evaluation of precancerous lesions could aid in early control of cancer. Heat shock protein (HSP) 27 has found to be a biomarker and therapeutic target in different types of cancer. AIM: This study aims to investigate the role of HSP 27 as prognostic molecular indicator of malignant transformation in oral epithelial dysplasias. MATERIALS AND METHODS: Thirty samples of epithelial dysplasia (10 mild dysplasia, 10 moderate dysplasia, and 10 severe dysplasia/carcinoma in situ cases), 10 samples each of well-differentiated OSCC and normal oral mucosa were routinely processed, formalin-fixed, paraffin-embedded, and analyzed for HSP27 expression by immunohistochemistry. Statistical analysis was done by one way-ANOVA and Mann–Whitney test to assess the differences between two individual groups. RESULTS: Normal mucosa showed intense, but nonuniform, expression of HSP27. An initial decline was noted in dysplasias. A significant correlation of HSP27 expression was observed with the severity of dysplasia and well-differentiated OSCC (P < 0.05). CONCLUSION: Low HSP 27 expression can be considered as early molecular indicator of initial dysplastic change in normal mucosa. An overexpression of HSP 27 in clinically and histologically confirmed dysplasia could indicate likely transformation to well-differentiated OSCC and could be of prognostic value. However, further studies with a larger sample size are required to confirm the role of HSP 27 as predictive indicator. Wolters Kluwer - Medknow 2020-05-12 /pmc/articles/PMC7365515/ /pubmed/32766120 http://dx.doi.org/10.4103/JMAU.JMAU_48_19 Text en Copyright: © 2020 Journal of Microscopy and Ultrastructure http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Karri, Roja Lakshmi Subramanyam, R. Venkata Venigella, Aparna Babburi, Suresh Pinisetti, Soujanya Rudraraju, Amrutha Differential Expression of Heat Shock Protein 27 in Oral Epithelial Dysplasias and Squamous Cell Carcinoma |
title | Differential Expression of Heat Shock Protein 27 in Oral Epithelial Dysplasias and Squamous Cell Carcinoma |
title_full | Differential Expression of Heat Shock Protein 27 in Oral Epithelial Dysplasias and Squamous Cell Carcinoma |
title_fullStr | Differential Expression of Heat Shock Protein 27 in Oral Epithelial Dysplasias and Squamous Cell Carcinoma |
title_full_unstemmed | Differential Expression of Heat Shock Protein 27 in Oral Epithelial Dysplasias and Squamous Cell Carcinoma |
title_short | Differential Expression of Heat Shock Protein 27 in Oral Epithelial Dysplasias and Squamous Cell Carcinoma |
title_sort | differential expression of heat shock protein 27 in oral epithelial dysplasias and squamous cell carcinoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365515/ https://www.ncbi.nlm.nih.gov/pubmed/32766120 http://dx.doi.org/10.4103/JMAU.JMAU_48_19 |
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