Combined Prophylactic and Therapeutic Use Maximizes Hydroxychloroquine Anti-SARS-CoV-2 Effects in vitro

While the SARS-CoV-2 pandemic is heavily hitting the world, it is of extreme importance that significant in vitro observations guide the quick set up of clinical trials. In this study, we evidence that the anti-SARS-CoV2 activity of a clinically achievable hydroxychloroquine concentration is maximiz...

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Autores principales: Clementi, Nicola, Criscuolo, Elena, Diotti, Roberta Antonia, Ferrarese, Roberto, Castelli, Matteo, Dagna, Lorenzo, Burioni, Roberto, Clementi, Massimo, Mancini, Nicasio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365881/
https://www.ncbi.nlm.nih.gov/pubmed/32754147
http://dx.doi.org/10.3389/fmicb.2020.01704
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author Clementi, Nicola
Criscuolo, Elena
Diotti, Roberta Antonia
Ferrarese, Roberto
Castelli, Matteo
Dagna, Lorenzo
Burioni, Roberto
Clementi, Massimo
Mancini, Nicasio
author_facet Clementi, Nicola
Criscuolo, Elena
Diotti, Roberta Antonia
Ferrarese, Roberto
Castelli, Matteo
Dagna, Lorenzo
Burioni, Roberto
Clementi, Massimo
Mancini, Nicasio
author_sort Clementi, Nicola
collection PubMed
description While the SARS-CoV-2 pandemic is heavily hitting the world, it is of extreme importance that significant in vitro observations guide the quick set up of clinical trials. In this study, we evidence that the anti-SARS-CoV2 activity of a clinically achievable hydroxychloroquine concentration is maximized only when administered before and after the infection of Vero E6 and Caco-2 cells. This suggests that only a combined prophylactic and therapeutic use of hydroxychloroquine may be effective in limiting viral replication in patients.
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spelling pubmed-73658812020-08-03 Combined Prophylactic and Therapeutic Use Maximizes Hydroxychloroquine Anti-SARS-CoV-2 Effects in vitro Clementi, Nicola Criscuolo, Elena Diotti, Roberta Antonia Ferrarese, Roberto Castelli, Matteo Dagna, Lorenzo Burioni, Roberto Clementi, Massimo Mancini, Nicasio Front Microbiol Microbiology While the SARS-CoV-2 pandemic is heavily hitting the world, it is of extreme importance that significant in vitro observations guide the quick set up of clinical trials. In this study, we evidence that the anti-SARS-CoV2 activity of a clinically achievable hydroxychloroquine concentration is maximized only when administered before and after the infection of Vero E6 and Caco-2 cells. This suggests that only a combined prophylactic and therapeutic use of hydroxychloroquine may be effective in limiting viral replication in patients. Frontiers Media S.A. 2020-07-10 /pmc/articles/PMC7365881/ /pubmed/32754147 http://dx.doi.org/10.3389/fmicb.2020.01704 Text en Copyright © 2020 Clementi, Criscuolo, Diotti, Ferrarese, Castelli, Dagna, Burioni, Clementi and Mancini. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Clementi, Nicola
Criscuolo, Elena
Diotti, Roberta Antonia
Ferrarese, Roberto
Castelli, Matteo
Dagna, Lorenzo
Burioni, Roberto
Clementi, Massimo
Mancini, Nicasio
Combined Prophylactic and Therapeutic Use Maximizes Hydroxychloroquine Anti-SARS-CoV-2 Effects in vitro
title Combined Prophylactic and Therapeutic Use Maximizes Hydroxychloroquine Anti-SARS-CoV-2 Effects in vitro
title_full Combined Prophylactic and Therapeutic Use Maximizes Hydroxychloroquine Anti-SARS-CoV-2 Effects in vitro
title_fullStr Combined Prophylactic and Therapeutic Use Maximizes Hydroxychloroquine Anti-SARS-CoV-2 Effects in vitro
title_full_unstemmed Combined Prophylactic and Therapeutic Use Maximizes Hydroxychloroquine Anti-SARS-CoV-2 Effects in vitro
title_short Combined Prophylactic and Therapeutic Use Maximizes Hydroxychloroquine Anti-SARS-CoV-2 Effects in vitro
title_sort combined prophylactic and therapeutic use maximizes hydroxychloroquine anti-sars-cov-2 effects in vitro
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365881/
https://www.ncbi.nlm.nih.gov/pubmed/32754147
http://dx.doi.org/10.3389/fmicb.2020.01704
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