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MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells

Periodontal ligament (PDL) stem cells (PDLSCs) have been reported as a useful cell source for periodontal tissue regeneration. However, one of the issues is the difficulty of obtaining a sufficient number of PDLSCs for clinical application because very few PDLSCs can be isolated from PDL tissue of d...

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Autores principales: Hasegawa, Daigaku, Hasegawa, Kana, Kaneko, Hiroshi, Yoshida, Shinichiro, Mitarai, Hiromi, Arima, Mai, Tomokiyo, Atsushi, Hamano, Sayuri, Sugii, Hideki, Wada, Naohisa, Kiyoshima, Tamotsu, Maeda, Hidefumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366229/
https://www.ncbi.nlm.nih.gov/pubmed/32724317
http://dx.doi.org/10.1155/2020/9672673
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author Hasegawa, Daigaku
Hasegawa, Kana
Kaneko, Hiroshi
Yoshida, Shinichiro
Mitarai, Hiromi
Arima, Mai
Tomokiyo, Atsushi
Hamano, Sayuri
Sugii, Hideki
Wada, Naohisa
Kiyoshima, Tamotsu
Maeda, Hidefumi
author_facet Hasegawa, Daigaku
Hasegawa, Kana
Kaneko, Hiroshi
Yoshida, Shinichiro
Mitarai, Hiromi
Arima, Mai
Tomokiyo, Atsushi
Hamano, Sayuri
Sugii, Hideki
Wada, Naohisa
Kiyoshima, Tamotsu
Maeda, Hidefumi
author_sort Hasegawa, Daigaku
collection PubMed
description Periodontal ligament (PDL) stem cells (PDLSCs) have been reported as a useful cell source for periodontal tissue regeneration. However, one of the issues is the difficulty of obtaining a sufficient number of PDLSCs for clinical application because very few PDLSCs can be isolated from PDL tissue of donors. Therefore, we aimed to identify a specific factor that converts human PDL cells into stem-like cells. In this study, microarray analysis comparing the gene profiles of human PDLSC lines (2-14 and 2-23) with those of a cell line with a low differentiation potential (2-52) identified the imprinted gene mesoderm-specific transcript (MEST). MEST was expressed in the cytoplasm of 2-23 cells. Knockdown of MEST by siRNA in 2-23 cells inhibited the expression of stem cell markers, such as CD105, CD146, p75NTR, N-cadherin, and NANOG; the proliferative potential; and multidifferentiation capacity for osteoblasts, adipocytes, and chondrocytes. On the other hand, overexpression of MEST in 2-52 cells enhanced the expression of stem cell markers and PDL-related markers and the multidifferentiation capacity. In addition, MEST-overexpressing 2-52 cells exhibited a change in morphology from a spindle shape to a stem cell-like round shape that was similar to 2-14 and 2-23 cell morphologies. These results suggest that MEST plays a critical role in the maintenance of stemness in PDLSCs and converts PDL cells into PDLSC-like cells. Therefore, this study indicates that MEST may be a therapeutic factor for periodontal tissue regeneration by inducing PDLSCs.
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spelling pubmed-73662292020-07-27 MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells Hasegawa, Daigaku Hasegawa, Kana Kaneko, Hiroshi Yoshida, Shinichiro Mitarai, Hiromi Arima, Mai Tomokiyo, Atsushi Hamano, Sayuri Sugii, Hideki Wada, Naohisa Kiyoshima, Tamotsu Maeda, Hidefumi Stem Cells Int Research Article Periodontal ligament (PDL) stem cells (PDLSCs) have been reported as a useful cell source for periodontal tissue regeneration. However, one of the issues is the difficulty of obtaining a sufficient number of PDLSCs for clinical application because very few PDLSCs can be isolated from PDL tissue of donors. Therefore, we aimed to identify a specific factor that converts human PDL cells into stem-like cells. In this study, microarray analysis comparing the gene profiles of human PDLSC lines (2-14 and 2-23) with those of a cell line with a low differentiation potential (2-52) identified the imprinted gene mesoderm-specific transcript (MEST). MEST was expressed in the cytoplasm of 2-23 cells. Knockdown of MEST by siRNA in 2-23 cells inhibited the expression of stem cell markers, such as CD105, CD146, p75NTR, N-cadherin, and NANOG; the proliferative potential; and multidifferentiation capacity for osteoblasts, adipocytes, and chondrocytes. On the other hand, overexpression of MEST in 2-52 cells enhanced the expression of stem cell markers and PDL-related markers and the multidifferentiation capacity. In addition, MEST-overexpressing 2-52 cells exhibited a change in morphology from a spindle shape to a stem cell-like round shape that was similar to 2-14 and 2-23 cell morphologies. These results suggest that MEST plays a critical role in the maintenance of stemness in PDLSCs and converts PDL cells into PDLSC-like cells. Therefore, this study indicates that MEST may be a therapeutic factor for periodontal tissue regeneration by inducing PDLSCs. Hindawi 2020-07-08 /pmc/articles/PMC7366229/ /pubmed/32724317 http://dx.doi.org/10.1155/2020/9672673 Text en Copyright © 2020 Daigaku Hasegawa et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hasegawa, Daigaku
Hasegawa, Kana
Kaneko, Hiroshi
Yoshida, Shinichiro
Mitarai, Hiromi
Arima, Mai
Tomokiyo, Atsushi
Hamano, Sayuri
Sugii, Hideki
Wada, Naohisa
Kiyoshima, Tamotsu
Maeda, Hidefumi
MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells
title MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells
title_full MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells
title_fullStr MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells
title_full_unstemmed MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells
title_short MEST Regulates the Stemness of Human Periodontal Ligament Stem Cells
title_sort mest regulates the stemness of human periodontal ligament stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366229/
https://www.ncbi.nlm.nih.gov/pubmed/32724317
http://dx.doi.org/10.1155/2020/9672673
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