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Temperature Dependent Control of the R27 Conjugative Plasmid Genes

Conjugation of R27 plasmid is thermoregulated, being promoted at 25°C and repressed at 37°C. Previous studies identified plasmid-encoded regulators, HtdA, TrhR and TrhY, that control expression of conjugation-related genes (tra). Moreover, the nucleoid-associated protein H-NS represses conjugation a...

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Autores principales: Gibert, Marta, Paytubi, Sonia, Madrid, Cristina, Balsalobre, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366339/
https://www.ncbi.nlm.nih.gov/pubmed/32754612
http://dx.doi.org/10.3389/fmolb.2020.00124
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author Gibert, Marta
Paytubi, Sonia
Madrid, Cristina
Balsalobre, Carlos
author_facet Gibert, Marta
Paytubi, Sonia
Madrid, Cristina
Balsalobre, Carlos
author_sort Gibert, Marta
collection PubMed
description Conjugation of R27 plasmid is thermoregulated, being promoted at 25°C and repressed at 37°C. Previous studies identified plasmid-encoded regulators, HtdA, TrhR and TrhY, that control expression of conjugation-related genes (tra). Moreover, the nucleoid-associated protein H-NS represses conjugation at non-permissive temperature. A transcriptomic approach has been used to characterize the effect of temperature on the expression of the 205 R27 genes. Many of the 35 tra genes, directly involved in plasmid-conjugation, were upregulated at 25°C. However, the majority of the non-tra R27 genes—many of them with unknown function—were more actively expressed at 37°C. The role of HtdA, a regulator that causes repression of the R27 conjugation by counteracting TrhR/TrhY mediated activation of tra genes, has been investigated. Most of the R27 genes are severely derepressed at 25°C in an htdA mutant, suggesting that HtdA is involved also in the repression of R27 genes other than the tra genes. Interestingly, the effect of htdA mutation was abolished at non-permissive temperature, indicating that the HtdA-TrhR/TrhY regulatory circuit mediates the environmental regulation of R27 gene expression. The role of H-NS in the proposed model is discussed.
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spelling pubmed-73663392020-08-03 Temperature Dependent Control of the R27 Conjugative Plasmid Genes Gibert, Marta Paytubi, Sonia Madrid, Cristina Balsalobre, Carlos Front Mol Biosci Molecular Biosciences Conjugation of R27 plasmid is thermoregulated, being promoted at 25°C and repressed at 37°C. Previous studies identified plasmid-encoded regulators, HtdA, TrhR and TrhY, that control expression of conjugation-related genes (tra). Moreover, the nucleoid-associated protein H-NS represses conjugation at non-permissive temperature. A transcriptomic approach has been used to characterize the effect of temperature on the expression of the 205 R27 genes. Many of the 35 tra genes, directly involved in plasmid-conjugation, were upregulated at 25°C. However, the majority of the non-tra R27 genes—many of them with unknown function—were more actively expressed at 37°C. The role of HtdA, a regulator that causes repression of the R27 conjugation by counteracting TrhR/TrhY mediated activation of tra genes, has been investigated. Most of the R27 genes are severely derepressed at 25°C in an htdA mutant, suggesting that HtdA is involved also in the repression of R27 genes other than the tra genes. Interestingly, the effect of htdA mutation was abolished at non-permissive temperature, indicating that the HtdA-TrhR/TrhY regulatory circuit mediates the environmental regulation of R27 gene expression. The role of H-NS in the proposed model is discussed. Frontiers Media S.A. 2020-07-10 /pmc/articles/PMC7366339/ /pubmed/32754612 http://dx.doi.org/10.3389/fmolb.2020.00124 Text en Copyright © 2020 Gibert, Paytubi, Madrid and Balsalobre. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Gibert, Marta
Paytubi, Sonia
Madrid, Cristina
Balsalobre, Carlos
Temperature Dependent Control of the R27 Conjugative Plasmid Genes
title Temperature Dependent Control of the R27 Conjugative Plasmid Genes
title_full Temperature Dependent Control of the R27 Conjugative Plasmid Genes
title_fullStr Temperature Dependent Control of the R27 Conjugative Plasmid Genes
title_full_unstemmed Temperature Dependent Control of the R27 Conjugative Plasmid Genes
title_short Temperature Dependent Control of the R27 Conjugative Plasmid Genes
title_sort temperature dependent control of the r27 conjugative plasmid genes
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366339/
https://www.ncbi.nlm.nih.gov/pubmed/32754612
http://dx.doi.org/10.3389/fmolb.2020.00124
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