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The novel features of Plantagoovata seed mucilage accumulation, storage and release

Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis. We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intac...

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Autores principales: Phan, Jana L., Cowley, James M., Neumann, Kylie A., Herliana, Lina, O’Donovan, Lisa A., Burton, Rachel A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366641/
https://www.ncbi.nlm.nih.gov/pubmed/32678191
http://dx.doi.org/10.1038/s41598-020-68685-w
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author Phan, Jana L.
Cowley, James M.
Neumann, Kylie A.
Herliana, Lina
O’Donovan, Lisa A.
Burton, Rachel A.
author_facet Phan, Jana L.
Cowley, James M.
Neumann, Kylie A.
Herliana, Lina
O’Donovan, Lisa A.
Burton, Rachel A.
author_sort Phan, Jana L.
collection PubMed
description Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis. We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae. Instead, dehydrated mucilage is spread in a thin homogenous layer over the entire seed surface and upon wetting expands directly outwards, away from the seed. Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored. The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to ‘jumpstart’ hydration and swelling of the second layer. This next layer, comprising the bulk of the expanded seed mucilage, is predominantly composed of heteroxylan and appears to provide much of the structural integrity. Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species.
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spelling pubmed-73666412020-07-17 The novel features of Plantagoovata seed mucilage accumulation, storage and release Phan, Jana L. Cowley, James M. Neumann, Kylie A. Herliana, Lina O’Donovan, Lisa A. Burton, Rachel A. Sci Rep Article Seed mucilage polysaccharide production, storage and release in Plantago ovata is strikingly different to that of the model plant Arabidopsis. We have used microscopy techniques to track the development of mucilage secretory cells and demonstrate that mature P. ovata seeds do not have an outer intact cell layer within which the polysaccharides surround internal columellae. Instead, dehydrated mucilage is spread in a thin homogenous layer over the entire seed surface and upon wetting expands directly outwards, away from the seed. Observing mucilage expansion in real time combined with compositional analysis allowed mucilage layer definition and the roles they play in mucilage release and architecture upon hydration to be explored. The first emergent layer of hydrated mucilage is rich in pectin, extremely hydrophilic, and forms an expansion front that functions to ‘jumpstart’ hydration and swelling of the second layer. This next layer, comprising the bulk of the expanded seed mucilage, is predominantly composed of heteroxylan and appears to provide much of the structural integrity. Our results indicate that the synthesis, deposition, desiccation, and final storage position of mucilage polysaccharides must be carefully orchestrated, although many of these processes are not yet fully defined and vary widely between myxospermous plant species. Nature Publishing Group UK 2020-07-16 /pmc/articles/PMC7366641/ /pubmed/32678191 http://dx.doi.org/10.1038/s41598-020-68685-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Phan, Jana L.
Cowley, James M.
Neumann, Kylie A.
Herliana, Lina
O’Donovan, Lisa A.
Burton, Rachel A.
The novel features of Plantagoovata seed mucilage accumulation, storage and release
title The novel features of Plantagoovata seed mucilage accumulation, storage and release
title_full The novel features of Plantagoovata seed mucilage accumulation, storage and release
title_fullStr The novel features of Plantagoovata seed mucilage accumulation, storage and release
title_full_unstemmed The novel features of Plantagoovata seed mucilage accumulation, storage and release
title_short The novel features of Plantagoovata seed mucilage accumulation, storage and release
title_sort novel features of plantagoovata seed mucilage accumulation, storage and release
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366641/
https://www.ncbi.nlm.nih.gov/pubmed/32678191
http://dx.doi.org/10.1038/s41598-020-68685-w
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