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Phosphorylation of ADP-Glucose Pyrophosphorylase During Wheat Seeds Development

Starch is the dominant reserve polysaccharide accumulated in the seed of grasses (like wheat). It is the most common carbohydrate in the human diet and a material applied to the bioplastics and biofuels industry. Hence, the complete understanding of starch metabolism is critical to design rational s...

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Autores principales: Ferrero, Danisa M. L., Piattoni, Claudia V., Asencion Diez, Matías D., Rojas, Bruno E., Hartman, Matías D., Ballicora, Miguel A., Iglesias, Alberto A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366821/
https://www.ncbi.nlm.nih.gov/pubmed/32754189
http://dx.doi.org/10.3389/fpls.2020.01058
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author Ferrero, Danisa M. L.
Piattoni, Claudia V.
Asencion Diez, Matías D.
Rojas, Bruno E.
Hartman, Matías D.
Ballicora, Miguel A.
Iglesias, Alberto A.
author_facet Ferrero, Danisa M. L.
Piattoni, Claudia V.
Asencion Diez, Matías D.
Rojas, Bruno E.
Hartman, Matías D.
Ballicora, Miguel A.
Iglesias, Alberto A.
author_sort Ferrero, Danisa M. L.
collection PubMed
description Starch is the dominant reserve polysaccharide accumulated in the seed of grasses (like wheat). It is the most common carbohydrate in the human diet and a material applied to the bioplastics and biofuels industry. Hence, the complete understanding of starch metabolism is critical to design rational strategies to improve its allocation in plant reserve tissues. ADP-glucose pyrophosphorylase (ADP-Glc PPase) catalyzes the key (regulated) step in the synthetic starch pathway. The enzyme comprises a small (S) and a large (L) subunit forming an S(2)L(2) heterotetramer, which is allosterically regulated by orthophosphate, fructose-6P, and 3P-glycerate. ADP-Glc PPase was found in a phosphorylated state in extracts from wheat seeds. The amount of the phosphorylated protein increased along with the development of the seed and correlated with relative increases of the enzyme activity and starch content. Conversely, this post-translational modification was absent in seeds from Ricinus communis. In vitro, the recombinant ADP-Glc PPase from wheat endosperm was phosphorylated by wheat seed extracts as well as by recombinant Ca(2+)-dependent plant protein kinases. Further analysis showed that the preferential phosphorylation takes place on the L subunit. Results suggest that the ADP-Glc PPase is a phosphorylation target in seeds from grasses but not from oleaginous plants. Accompanying seed maturation and starch accumulation, a combined regulation of ADP-Glc PPase by metabolites and phosphorylation may provide an enzyme with stable levels of activity. Such concerted modulation would drive carbon skeletons to the synthesis of starch for its long-term storage, which later support seed germination.
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spelling pubmed-73668212020-08-03 Phosphorylation of ADP-Glucose Pyrophosphorylase During Wheat Seeds Development Ferrero, Danisa M. L. Piattoni, Claudia V. Asencion Diez, Matías D. Rojas, Bruno E. Hartman, Matías D. Ballicora, Miguel A. Iglesias, Alberto A. Front Plant Sci Plant Science Starch is the dominant reserve polysaccharide accumulated in the seed of grasses (like wheat). It is the most common carbohydrate in the human diet and a material applied to the bioplastics and biofuels industry. Hence, the complete understanding of starch metabolism is critical to design rational strategies to improve its allocation in plant reserve tissues. ADP-glucose pyrophosphorylase (ADP-Glc PPase) catalyzes the key (regulated) step in the synthetic starch pathway. The enzyme comprises a small (S) and a large (L) subunit forming an S(2)L(2) heterotetramer, which is allosterically regulated by orthophosphate, fructose-6P, and 3P-glycerate. ADP-Glc PPase was found in a phosphorylated state in extracts from wheat seeds. The amount of the phosphorylated protein increased along with the development of the seed and correlated with relative increases of the enzyme activity and starch content. Conversely, this post-translational modification was absent in seeds from Ricinus communis. In vitro, the recombinant ADP-Glc PPase from wheat endosperm was phosphorylated by wheat seed extracts as well as by recombinant Ca(2+)-dependent plant protein kinases. Further analysis showed that the preferential phosphorylation takes place on the L subunit. Results suggest that the ADP-Glc PPase is a phosphorylation target in seeds from grasses but not from oleaginous plants. Accompanying seed maturation and starch accumulation, a combined regulation of ADP-Glc PPase by metabolites and phosphorylation may provide an enzyme with stable levels of activity. Such concerted modulation would drive carbon skeletons to the synthesis of starch for its long-term storage, which later support seed germination. Frontiers Media S.A. 2020-07-10 /pmc/articles/PMC7366821/ /pubmed/32754189 http://dx.doi.org/10.3389/fpls.2020.01058 Text en Copyright © 2020 Ferrero, Piattoni, Asencion Diez, Rojas, Hartman, Ballicora and Iglesias http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Ferrero, Danisa M. L.
Piattoni, Claudia V.
Asencion Diez, Matías D.
Rojas, Bruno E.
Hartman, Matías D.
Ballicora, Miguel A.
Iglesias, Alberto A.
Phosphorylation of ADP-Glucose Pyrophosphorylase During Wheat Seeds Development
title Phosphorylation of ADP-Glucose Pyrophosphorylase During Wheat Seeds Development
title_full Phosphorylation of ADP-Glucose Pyrophosphorylase During Wheat Seeds Development
title_fullStr Phosphorylation of ADP-Glucose Pyrophosphorylase During Wheat Seeds Development
title_full_unstemmed Phosphorylation of ADP-Glucose Pyrophosphorylase During Wheat Seeds Development
title_short Phosphorylation of ADP-Glucose Pyrophosphorylase During Wheat Seeds Development
title_sort phosphorylation of adp-glucose pyrophosphorylase during wheat seeds development
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7366821/
https://www.ncbi.nlm.nih.gov/pubmed/32754189
http://dx.doi.org/10.3389/fpls.2020.01058
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