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Response of Brassica napus to Plasmodiophora brassicae Involves Salicylic Acid-Mediated Immunity: An RNA-Seq-Based Study

Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is an important disease of the Brassicaceae and poses a significant threat to the $26.7 billion canola/oilseed rape (Brassica napus) industry in western Canada. While clubroot is managed most effectively by planting resistant host v...

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Autores principales: Galindo-González, Leonardo, Manolii, Victor, Hwang, Sheau-Fang, Strelkov, Stephen E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367028/
https://www.ncbi.nlm.nih.gov/pubmed/32754180
http://dx.doi.org/10.3389/fpls.2020.01025
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author Galindo-González, Leonardo
Manolii, Victor
Hwang, Sheau-Fang
Strelkov, Stephen E.
author_facet Galindo-González, Leonardo
Manolii, Victor
Hwang, Sheau-Fang
Strelkov, Stephen E.
author_sort Galindo-González, Leonardo
collection PubMed
description Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is an important disease of the Brassicaceae and poses a significant threat to the $26.7 billion canola/oilseed rape (Brassica napus) industry in western Canada. While clubroot is managed most effectively by planting resistant host varieties, new pathotypes of P. brassicae have emerged recently that can overcome this resistance. Whole genome analyses provide both a toolbox and a systemic view of molecular mechanisms in host-pathogen interactions, which can be used to design new breeding strategies to increase P. brassicae resistance. We used RNA-seq to evaluate differential gene expression at 7, 14 and 21 days after inoculation (dai) of two B. napus genotypes with differential responses to P. brassicae pathotype 5X. Gall development was evident at 14 dai in the susceptible genotype (the oilseed rape ‘Brutor’), while gall development in the resistant genotype (the rutabaga (B. napus) ‘Laurentian’) was limited and not visible until 21 dai. Immune responses were better sustained through the time-course in ‘Laurentian’, and numerous genes from immune-related functional categories were associated with salicylic acid (SA)-mediated responses. Jasmonic acid (JA)-mediated responses seemed to be mostly inhibited, especially in the resistant genotype. The upregulation of standard defense-related proteins, like chitinases and thaumatins, was evident in ‘Laurentian’. The enrichment, in both host genotypes, of functional categories for syncytium formation and response to nematodes indicated that cell enlargement during P. brassicae infection, and the metabolic processes therein, share similarities with the response to infection by nematodes that produce similar anatomical symptoms. An analysis of shared genes between the two genotypes at different time-points, confirmed that the nematode-like responses occurred earlier for ‘Brutor’, along with cell metabolism and growth changes. Additionally, the susceptible cultivar turned off defense mechanisms earlier than ‘Laurentian’. Collectively, this study showed the importance of SA in triggering immune responses and suggested some key resistance and susceptibility factors that can be used in future studies for resistance breeding through gene-editing approaches.
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spelling pubmed-73670282020-08-03 Response of Brassica napus to Plasmodiophora brassicae Involves Salicylic Acid-Mediated Immunity: An RNA-Seq-Based Study Galindo-González, Leonardo Manolii, Victor Hwang, Sheau-Fang Strelkov, Stephen E. Front Plant Sci Plant Science Clubroot, caused by the obligate parasite Plasmodiophora brassicae, is an important disease of the Brassicaceae and poses a significant threat to the $26.7 billion canola/oilseed rape (Brassica napus) industry in western Canada. While clubroot is managed most effectively by planting resistant host varieties, new pathotypes of P. brassicae have emerged recently that can overcome this resistance. Whole genome analyses provide both a toolbox and a systemic view of molecular mechanisms in host-pathogen interactions, which can be used to design new breeding strategies to increase P. brassicae resistance. We used RNA-seq to evaluate differential gene expression at 7, 14 and 21 days after inoculation (dai) of two B. napus genotypes with differential responses to P. brassicae pathotype 5X. Gall development was evident at 14 dai in the susceptible genotype (the oilseed rape ‘Brutor’), while gall development in the resistant genotype (the rutabaga (B. napus) ‘Laurentian’) was limited and not visible until 21 dai. Immune responses were better sustained through the time-course in ‘Laurentian’, and numerous genes from immune-related functional categories were associated with salicylic acid (SA)-mediated responses. Jasmonic acid (JA)-mediated responses seemed to be mostly inhibited, especially in the resistant genotype. The upregulation of standard defense-related proteins, like chitinases and thaumatins, was evident in ‘Laurentian’. The enrichment, in both host genotypes, of functional categories for syncytium formation and response to nematodes indicated that cell enlargement during P. brassicae infection, and the metabolic processes therein, share similarities with the response to infection by nematodes that produce similar anatomical symptoms. An analysis of shared genes between the two genotypes at different time-points, confirmed that the nematode-like responses occurred earlier for ‘Brutor’, along with cell metabolism and growth changes. Additionally, the susceptible cultivar turned off defense mechanisms earlier than ‘Laurentian’. Collectively, this study showed the importance of SA in triggering immune responses and suggested some key resistance and susceptibility factors that can be used in future studies for resistance breeding through gene-editing approaches. Frontiers Media S.A. 2020-07-09 /pmc/articles/PMC7367028/ /pubmed/32754180 http://dx.doi.org/10.3389/fpls.2020.01025 Text en Copyright © 2020 Galindo-González, Manolii, Hwang and Strelkov http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Galindo-González, Leonardo
Manolii, Victor
Hwang, Sheau-Fang
Strelkov, Stephen E.
Response of Brassica napus to Plasmodiophora brassicae Involves Salicylic Acid-Mediated Immunity: An RNA-Seq-Based Study
title Response of Brassica napus to Plasmodiophora brassicae Involves Salicylic Acid-Mediated Immunity: An RNA-Seq-Based Study
title_full Response of Brassica napus to Plasmodiophora brassicae Involves Salicylic Acid-Mediated Immunity: An RNA-Seq-Based Study
title_fullStr Response of Brassica napus to Plasmodiophora brassicae Involves Salicylic Acid-Mediated Immunity: An RNA-Seq-Based Study
title_full_unstemmed Response of Brassica napus to Plasmodiophora brassicae Involves Salicylic Acid-Mediated Immunity: An RNA-Seq-Based Study
title_short Response of Brassica napus to Plasmodiophora brassicae Involves Salicylic Acid-Mediated Immunity: An RNA-Seq-Based Study
title_sort response of brassica napus to plasmodiophora brassicae involves salicylic acid-mediated immunity: an rna-seq-based study
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367028/
https://www.ncbi.nlm.nih.gov/pubmed/32754180
http://dx.doi.org/10.3389/fpls.2020.01025
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