Cargando…

Antimicrobial activity of mesenchymal stem cells against Staphylococcus aureus

INTRODUCTION: There have been limited advances in the treatment of bone and joint infections, which currently involves a combination of surgery and antibiotic administration. There is a timely need in orthopedics to develop more effective and less invasive forms of antimicrobial prophylaxis and trea...

Descripción completa

Detalles Bibliográficos
Autores principales: Yagi, Haruyo, Chen, Antonia F., Hirsch, David, Rothenberg, Adam C., Tan, Jian, Alexander, Peter G., Tuan, Rocky S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367313/
https://www.ncbi.nlm.nih.gov/pubmed/32680544
http://dx.doi.org/10.1186/s13287-020-01807-3
_version_ 1783560399596027904
author Yagi, Haruyo
Chen, Antonia F.
Hirsch, David
Rothenberg, Adam C.
Tan, Jian
Alexander, Peter G.
Tuan, Rocky S.
author_facet Yagi, Haruyo
Chen, Antonia F.
Hirsch, David
Rothenberg, Adam C.
Tan, Jian
Alexander, Peter G.
Tuan, Rocky S.
author_sort Yagi, Haruyo
collection PubMed
description INTRODUCTION: There have been limited advances in the treatment of bone and joint infections, which currently involves a combination of surgery and antibiotic administration. There is a timely need in orthopedics to develop more effective and less invasive forms of antimicrobial prophylaxis and treatment. The antibacterial effect of adult tissue-derived mesenchymal stem cells (MSCs) has recently been investigated against Escherichia coli and Staphylococcus aureus. The main mechanism of action is postulated to be via MSC production of the cationic antimicrobial peptide, LL-37. METHODS: This study examines the antimicrobial activity of adipose-derived human MSCs (ASCs) on S. aureus, specifically examining the role of LL-37 and regulation of its expression. Bacteria colony-forming unit (CFU) assay was used to assess antimicrobial activity. RESULTS: Our results showed that the ASC-conditioned medium significantly inhibited the growth of S. aureus under standard culture conditions with or without the continued presence of ASCs. Also, the treatment of ASCs with 1,25-dihydroxy vitamin D(3) elevated LL-37 expression and enhanced their antimicrobial activity. In support, treatment with the vitamin D receptor inhibitor, GW0742, blocked the antimicrobial activity of ASCs. CONCLUSION: Our findings clearly demonstrate the antimicrobial activity of adult ASCs against S. aureus and implicate a key regulatory role for vitamin D. Further testing in in vivo models is being pursued to assess the potential application of ASCs as a biocompatible, adjunct treatment for musculoskeletal infections.
format Online
Article
Text
id pubmed-7367313
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-73673132020-07-20 Antimicrobial activity of mesenchymal stem cells against Staphylococcus aureus Yagi, Haruyo Chen, Antonia F. Hirsch, David Rothenberg, Adam C. Tan, Jian Alexander, Peter G. Tuan, Rocky S. Stem Cell Res Ther Research INTRODUCTION: There have been limited advances in the treatment of bone and joint infections, which currently involves a combination of surgery and antibiotic administration. There is a timely need in orthopedics to develop more effective and less invasive forms of antimicrobial prophylaxis and treatment. The antibacterial effect of adult tissue-derived mesenchymal stem cells (MSCs) has recently been investigated against Escherichia coli and Staphylococcus aureus. The main mechanism of action is postulated to be via MSC production of the cationic antimicrobial peptide, LL-37. METHODS: This study examines the antimicrobial activity of adipose-derived human MSCs (ASCs) on S. aureus, specifically examining the role of LL-37 and regulation of its expression. Bacteria colony-forming unit (CFU) assay was used to assess antimicrobial activity. RESULTS: Our results showed that the ASC-conditioned medium significantly inhibited the growth of S. aureus under standard culture conditions with or without the continued presence of ASCs. Also, the treatment of ASCs with 1,25-dihydroxy vitamin D(3) elevated LL-37 expression and enhanced their antimicrobial activity. In support, treatment with the vitamin D receptor inhibitor, GW0742, blocked the antimicrobial activity of ASCs. CONCLUSION: Our findings clearly demonstrate the antimicrobial activity of adult ASCs against S. aureus and implicate a key regulatory role for vitamin D. Further testing in in vivo models is being pursued to assess the potential application of ASCs as a biocompatible, adjunct treatment for musculoskeletal infections. BioMed Central 2020-07-17 /pmc/articles/PMC7367313/ /pubmed/32680544 http://dx.doi.org/10.1186/s13287-020-01807-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Yagi, Haruyo
Chen, Antonia F.
Hirsch, David
Rothenberg, Adam C.
Tan, Jian
Alexander, Peter G.
Tuan, Rocky S.
Antimicrobial activity of mesenchymal stem cells against Staphylococcus aureus
title Antimicrobial activity of mesenchymal stem cells against Staphylococcus aureus
title_full Antimicrobial activity of mesenchymal stem cells against Staphylococcus aureus
title_fullStr Antimicrobial activity of mesenchymal stem cells against Staphylococcus aureus
title_full_unstemmed Antimicrobial activity of mesenchymal stem cells against Staphylococcus aureus
title_short Antimicrobial activity of mesenchymal stem cells against Staphylococcus aureus
title_sort antimicrobial activity of mesenchymal stem cells against staphylococcus aureus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367313/
https://www.ncbi.nlm.nih.gov/pubmed/32680544
http://dx.doi.org/10.1186/s13287-020-01807-3
work_keys_str_mv AT yagiharuyo antimicrobialactivityofmesenchymalstemcellsagainststaphylococcusaureus
AT chenantoniaf antimicrobialactivityofmesenchymalstemcellsagainststaphylococcusaureus
AT hirschdavid antimicrobialactivityofmesenchymalstemcellsagainststaphylococcusaureus
AT rothenbergadamc antimicrobialactivityofmesenchymalstemcellsagainststaphylococcusaureus
AT tanjian antimicrobialactivityofmesenchymalstemcellsagainststaphylococcusaureus
AT alexanderpeterg antimicrobialactivityofmesenchymalstemcellsagainststaphylococcusaureus
AT tuanrockys antimicrobialactivityofmesenchymalstemcellsagainststaphylococcusaureus