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VOC fingerprints: metabolomic signatures of biothreat agents with and without antibiotic resistance

Category A and B biothreat agents are deemed to be of great concern by the US Centers for Disease Control and Prevention (CDC) and include the bacteria Francisella tularensis, Yersinia pestis, Burkholderia mallei, and Brucella species. Underscored by the impact of the 2020 SARS-CoV-2 outbreak, 2016...

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Autores principales: Dailey, Allyson, Saha, Jessica, Zaidi, Fatima, Abdirahman, Hafsa, Haymond, Amanda, Alem, Farhang, Hakami, Ramin, Couch, Robin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367350/
https://www.ncbi.nlm.nih.gov/pubmed/32678173
http://dx.doi.org/10.1038/s41598-020-68622-x
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author Dailey, Allyson
Saha, Jessica
Zaidi, Fatima
Abdirahman, Hafsa
Haymond, Amanda
Alem, Farhang
Hakami, Ramin
Couch, Robin
author_facet Dailey, Allyson
Saha, Jessica
Zaidi, Fatima
Abdirahman, Hafsa
Haymond, Amanda
Alem, Farhang
Hakami, Ramin
Couch, Robin
author_sort Dailey, Allyson
collection PubMed
description Category A and B biothreat agents are deemed to be of great concern by the US Centers for Disease Control and Prevention (CDC) and include the bacteria Francisella tularensis, Yersinia pestis, Burkholderia mallei, and Brucella species. Underscored by the impact of the 2020 SARS-CoV-2 outbreak, 2016 Zika pandemic, 2014 Ebola outbreak, 2001 anthrax letter attacks, and 1984 Rajneeshee Salmonella attacks, the threat of future epidemics/pandemics and/or terrorist/criminal use of pathogenic organisms warrants continued exploration and development of both classic and alternative methods of detecting biothreat agents. Volatile organic compounds (VOCs) comprise a large and highly diverse group of carbon-based molecules, generally related by their volatility at ambient temperature. Recently, the diagnostic potential of VOCs has been realized, as correlations between the microbial VOC metabolome and specific bacterial pathogens have been identified. Herein, we describe the use of microbial VOC profiles as fingerprints for the identification of biothreat-relevant microbes, and for differentiating between a kanamycin susceptible and resistant strain. Additionally, we demonstrate microbial VOC profiling using a rapid-throughput VOC metabolomics method we refer to as ‘simultaneous multifiber headspace solid-phase microextraction’ (simulti-hSPME). Finally, through VOC analysis, we illustrate a rapid non-invasive approach to the diagnosis of BALB/c mice infected with either F. tularensis SCHU S4 or Y. pestis CO92.
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spelling pubmed-73673502020-07-20 VOC fingerprints: metabolomic signatures of biothreat agents with and without antibiotic resistance Dailey, Allyson Saha, Jessica Zaidi, Fatima Abdirahman, Hafsa Haymond, Amanda Alem, Farhang Hakami, Ramin Couch, Robin Sci Rep Article Category A and B biothreat agents are deemed to be of great concern by the US Centers for Disease Control and Prevention (CDC) and include the bacteria Francisella tularensis, Yersinia pestis, Burkholderia mallei, and Brucella species. Underscored by the impact of the 2020 SARS-CoV-2 outbreak, 2016 Zika pandemic, 2014 Ebola outbreak, 2001 anthrax letter attacks, and 1984 Rajneeshee Salmonella attacks, the threat of future epidemics/pandemics and/or terrorist/criminal use of pathogenic organisms warrants continued exploration and development of both classic and alternative methods of detecting biothreat agents. Volatile organic compounds (VOCs) comprise a large and highly diverse group of carbon-based molecules, generally related by their volatility at ambient temperature. Recently, the diagnostic potential of VOCs has been realized, as correlations between the microbial VOC metabolome and specific bacterial pathogens have been identified. Herein, we describe the use of microbial VOC profiles as fingerprints for the identification of biothreat-relevant microbes, and for differentiating between a kanamycin susceptible and resistant strain. Additionally, we demonstrate microbial VOC profiling using a rapid-throughput VOC metabolomics method we refer to as ‘simultaneous multifiber headspace solid-phase microextraction’ (simulti-hSPME). Finally, through VOC analysis, we illustrate a rapid non-invasive approach to the diagnosis of BALB/c mice infected with either F. tularensis SCHU S4 or Y. pestis CO92. Nature Publishing Group UK 2020-07-16 /pmc/articles/PMC7367350/ /pubmed/32678173 http://dx.doi.org/10.1038/s41598-020-68622-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dailey, Allyson
Saha, Jessica
Zaidi, Fatima
Abdirahman, Hafsa
Haymond, Amanda
Alem, Farhang
Hakami, Ramin
Couch, Robin
VOC fingerprints: metabolomic signatures of biothreat agents with and without antibiotic resistance
title VOC fingerprints: metabolomic signatures of biothreat agents with and without antibiotic resistance
title_full VOC fingerprints: metabolomic signatures of biothreat agents with and without antibiotic resistance
title_fullStr VOC fingerprints: metabolomic signatures of biothreat agents with and without antibiotic resistance
title_full_unstemmed VOC fingerprints: metabolomic signatures of biothreat agents with and without antibiotic resistance
title_short VOC fingerprints: metabolomic signatures of biothreat agents with and without antibiotic resistance
title_sort voc fingerprints: metabolomic signatures of biothreat agents with and without antibiotic resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367350/
https://www.ncbi.nlm.nih.gov/pubmed/32678173
http://dx.doi.org/10.1038/s41598-020-68622-x
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