Cargando…
EtMIC3 and its receptors BAG1 and ENDOUL are essential for site-specific invasion of Eimeria tenella in chickens
Avian coccidian parasites exhibit a high degree of site specificity in different Eimeria species. Although the underlying mechanism is unclear, an increasing body of evidence suggests that site specificity is due to the interaction between microneme proteins (MICs) and their receptors on the surface...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367391/ https://www.ncbi.nlm.nih.gov/pubmed/32678057 http://dx.doi.org/10.1186/s13567-020-00809-6 |
_version_ | 1783560416896483328 |
---|---|
author | Li, Wenyu Wang, Mingyue Chen, Yufeng Chen, Chen Liu, Xiaoqian Sun, Xiaoting Jing, Chuanxu Xu, Lixin Yan, Ruofeng Li, Xiangrui Song, Xiaokai |
author_facet | Li, Wenyu Wang, Mingyue Chen, Yufeng Chen, Chen Liu, Xiaoqian Sun, Xiaoting Jing, Chuanxu Xu, Lixin Yan, Ruofeng Li, Xiangrui Song, Xiaokai |
author_sort | Li, Wenyu |
collection | PubMed |
description | Avian coccidian parasites exhibit a high degree of site specificity in different Eimeria species. Although the underlying mechanism is unclear, an increasing body of evidence suggests that site specificity is due to the interaction between microneme proteins (MICs) and their receptors on the surface of target host cells. In this study, the binding ability of E. tenella MICs (EtMICs) to different intestinal tissue was observed by immunofluorescence to identify the key surface molecule on the parasite responsible for the site specificity. Subsequently, we identified the corresponding host-cell receptors by yeast two-hybrid screening and glutathione-S-transferase pull-down experiments and the distribution of these receptors was observed by immunofluorescence in chicken intestinal tissues. Finally, we evaluated the efficacy of receptor antiserum against the infection of E. tenella in chickens. The results showed that EtMIC3 could only bind to the caecum while EtMIC1, EtMIC2, and EtAMA1 did not bind to any other intestinal tissues. Anti-serum to EtMIC3 was able to block the invasion of sporozoites with a blocking rate of 66.3%. The receptors for EtMIC3 were BCL2-associated athanogene 1 (BAG1) and Endonuclease polyU-specific-like (ENDOUL), which were mainly distributed in the caecum. BAG1 and ENDOUL receptor antiserum reduced weight loss and oocyst output following E. tenella infection, showing partial inhibition of E. tenella infection. These data elucidate the mechanism of site specificity for Eimeria infection and reveal a potential therapeutic avenue. |
format | Online Article Text |
id | pubmed-7367391 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-73673912020-07-20 EtMIC3 and its receptors BAG1 and ENDOUL are essential for site-specific invasion of Eimeria tenella in chickens Li, Wenyu Wang, Mingyue Chen, Yufeng Chen, Chen Liu, Xiaoqian Sun, Xiaoting Jing, Chuanxu Xu, Lixin Yan, Ruofeng Li, Xiangrui Song, Xiaokai Vet Res Research Article Avian coccidian parasites exhibit a high degree of site specificity in different Eimeria species. Although the underlying mechanism is unclear, an increasing body of evidence suggests that site specificity is due to the interaction between microneme proteins (MICs) and their receptors on the surface of target host cells. In this study, the binding ability of E. tenella MICs (EtMICs) to different intestinal tissue was observed by immunofluorescence to identify the key surface molecule on the parasite responsible for the site specificity. Subsequently, we identified the corresponding host-cell receptors by yeast two-hybrid screening and glutathione-S-transferase pull-down experiments and the distribution of these receptors was observed by immunofluorescence in chicken intestinal tissues. Finally, we evaluated the efficacy of receptor antiserum against the infection of E. tenella in chickens. The results showed that EtMIC3 could only bind to the caecum while EtMIC1, EtMIC2, and EtAMA1 did not bind to any other intestinal tissues. Anti-serum to EtMIC3 was able to block the invasion of sporozoites with a blocking rate of 66.3%. The receptors for EtMIC3 were BCL2-associated athanogene 1 (BAG1) and Endonuclease polyU-specific-like (ENDOUL), which were mainly distributed in the caecum. BAG1 and ENDOUL receptor antiserum reduced weight loss and oocyst output following E. tenella infection, showing partial inhibition of E. tenella infection. These data elucidate the mechanism of site specificity for Eimeria infection and reveal a potential therapeutic avenue. BioMed Central 2020-07-16 2020 /pmc/articles/PMC7367391/ /pubmed/32678057 http://dx.doi.org/10.1186/s13567-020-00809-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Li, Wenyu Wang, Mingyue Chen, Yufeng Chen, Chen Liu, Xiaoqian Sun, Xiaoting Jing, Chuanxu Xu, Lixin Yan, Ruofeng Li, Xiangrui Song, Xiaokai EtMIC3 and its receptors BAG1 and ENDOUL are essential for site-specific invasion of Eimeria tenella in chickens |
title | EtMIC3 and its receptors BAG1 and ENDOUL are essential for site-specific invasion of Eimeria tenella in chickens |
title_full | EtMIC3 and its receptors BAG1 and ENDOUL are essential for site-specific invasion of Eimeria tenella in chickens |
title_fullStr | EtMIC3 and its receptors BAG1 and ENDOUL are essential for site-specific invasion of Eimeria tenella in chickens |
title_full_unstemmed | EtMIC3 and its receptors BAG1 and ENDOUL are essential for site-specific invasion of Eimeria tenella in chickens |
title_short | EtMIC3 and its receptors BAG1 and ENDOUL are essential for site-specific invasion of Eimeria tenella in chickens |
title_sort | etmic3 and its receptors bag1 and endoul are essential for site-specific invasion of eimeria tenella in chickens |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367391/ https://www.ncbi.nlm.nih.gov/pubmed/32678057 http://dx.doi.org/10.1186/s13567-020-00809-6 |
work_keys_str_mv | AT liwenyu etmic3anditsreceptorsbag1andendoulareessentialforsitespecificinvasionofeimeriatenellainchickens AT wangmingyue etmic3anditsreceptorsbag1andendoulareessentialforsitespecificinvasionofeimeriatenellainchickens AT chenyufeng etmic3anditsreceptorsbag1andendoulareessentialforsitespecificinvasionofeimeriatenellainchickens AT chenchen etmic3anditsreceptorsbag1andendoulareessentialforsitespecificinvasionofeimeriatenellainchickens AT liuxiaoqian etmic3anditsreceptorsbag1andendoulareessentialforsitespecificinvasionofeimeriatenellainchickens AT sunxiaoting etmic3anditsreceptorsbag1andendoulareessentialforsitespecificinvasionofeimeriatenellainchickens AT jingchuanxu etmic3anditsreceptorsbag1andendoulareessentialforsitespecificinvasionofeimeriatenellainchickens AT xulixin etmic3anditsreceptorsbag1andendoulareessentialforsitespecificinvasionofeimeriatenellainchickens AT yanruofeng etmic3anditsreceptorsbag1andendoulareessentialforsitespecificinvasionofeimeriatenellainchickens AT lixiangrui etmic3anditsreceptorsbag1andendoulareessentialforsitespecificinvasionofeimeriatenellainchickens AT songxiaokai etmic3anditsreceptorsbag1andendoulareessentialforsitespecificinvasionofeimeriatenellainchickens |