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Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti

BACKGROUND: The wide distribution of Aedes aegypti, the main vector of dengue and yellow fever viruses, currently puts three billion people in the world at risk of infection with these viruses. Continuous transmission of these and other viruses despite aggressive efforts to prevent this emphasizes t...

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Autores principales: Kojin, Bianca B., Biedler, James K., Tu, Zhijian, Adelman, Zach N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367395/
https://www.ncbi.nlm.nih.gov/pubmed/32680549
http://dx.doi.org/10.1186/s13071-020-04216-w
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author Kojin, Bianca B.
Biedler, James K.
Tu, Zhijian
Adelman, Zach N.
author_facet Kojin, Bianca B.
Biedler, James K.
Tu, Zhijian
Adelman, Zach N.
author_sort Kojin, Bianca B.
collection PubMed
description BACKGROUND: The wide distribution of Aedes aegypti, the main vector of dengue and yellow fever viruses, currently puts three billion people in the world at risk of infection with these viruses. Continuous transmission of these and other viruses despite aggressive efforts to prevent this emphasizes the need to develop new control strategies. Proposals to control disease transmission based on vector engineering, including both population suppression and population replacement, rely on the development of transgenes under the control of regulatory elements able to drive molecules in a specific tissue, time and strength. METHODS: Here we report the characterization of a promoter active in both the female germline and early zygote, derived from the transcription factor bZip1 in the mosquito Ae. aegypti, using transposon-based methods and RT-qPCR. RESULTS: We generated seven transgenic lines carrying AabZip1-reporter constructs and observed expression in both the ovary and early embryo. RT-qPCR analysis was performed to evaluate transcript expression patterns for each line, confirming that transgenic expression from the AabZip1 promoter largely recapitulated the endogenous expression pattern, albeit the strength of maternal expression appeared to be strongly influenced by chromosomal position. CONCLUSIONS: This study provides a new regulatory sequence that can be useful for generating transgenic lines that can become a tool in vector control strategies. [Image: see text]
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spelling pubmed-73673952020-07-20 Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti Kojin, Bianca B. Biedler, James K. Tu, Zhijian Adelman, Zach N. Parasit Vectors Research BACKGROUND: The wide distribution of Aedes aegypti, the main vector of dengue and yellow fever viruses, currently puts three billion people in the world at risk of infection with these viruses. Continuous transmission of these and other viruses despite aggressive efforts to prevent this emphasizes the need to develop new control strategies. Proposals to control disease transmission based on vector engineering, including both population suppression and population replacement, rely on the development of transgenes under the control of regulatory elements able to drive molecules in a specific tissue, time and strength. METHODS: Here we report the characterization of a promoter active in both the female germline and early zygote, derived from the transcription factor bZip1 in the mosquito Ae. aegypti, using transposon-based methods and RT-qPCR. RESULTS: We generated seven transgenic lines carrying AabZip1-reporter constructs and observed expression in both the ovary and early embryo. RT-qPCR analysis was performed to evaluate transcript expression patterns for each line, confirming that transgenic expression from the AabZip1 promoter largely recapitulated the endogenous expression pattern, albeit the strength of maternal expression appeared to be strongly influenced by chromosomal position. CONCLUSIONS: This study provides a new regulatory sequence that can be useful for generating transgenic lines that can become a tool in vector control strategies. [Image: see text] BioMed Central 2020-07-17 /pmc/articles/PMC7367395/ /pubmed/32680549 http://dx.doi.org/10.1186/s13071-020-04216-w Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kojin, Bianca B.
Biedler, James K.
Tu, Zhijian
Adelman, Zach N.
Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti
title Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti
title_full Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti
title_fullStr Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti
title_full_unstemmed Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti
title_short Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti
title_sort characterization of a female germline and early zygote promoter from the transcription factor bzip1 in the dengue mosquito aedes aegypti
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367395/
https://www.ncbi.nlm.nih.gov/pubmed/32680549
http://dx.doi.org/10.1186/s13071-020-04216-w
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