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Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans

BACKGROUND: Sweet osmanthus (Osmanthus fragrans Lour.) is one of the top ten traditional ornamental flowers in China. The flowering time of once-flowering cultivars in O. fragrans is greatly affected by the relatively low temperature, but there are few reports on its molecular mechanism to date. A h...

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Autores principales: Fu, Jianxin, Zhang, Chao, Liu, Yucheng, Pang, Tianhong, Dong, Bin, Gao, Xiaoyue, Zhu, Yimin, Zhao, Hongbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367400/
https://www.ncbi.nlm.nih.gov/pubmed/32677959
http://dx.doi.org/10.1186/s12870-020-02549-3
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author Fu, Jianxin
Zhang, Chao
Liu, Yucheng
Pang, Tianhong
Dong, Bin
Gao, Xiaoyue
Zhu, Yimin
Zhao, Hongbo
author_facet Fu, Jianxin
Zhang, Chao
Liu, Yucheng
Pang, Tianhong
Dong, Bin
Gao, Xiaoyue
Zhu, Yimin
Zhao, Hongbo
author_sort Fu, Jianxin
collection PubMed
description BACKGROUND: Sweet osmanthus (Osmanthus fragrans Lour.) is one of the top ten traditional ornamental flowers in China. The flowering time of once-flowering cultivars in O. fragrans is greatly affected by the relatively low temperature, but there are few reports on its molecular mechanism to date. A hypothesis had been raised that genes related with flower opening might be up-regulated in response to relatively low temperature in O. fragrans. Thus, our work was aimed to explore the underlying molecular mechanism of flower opening regulated by relatively low temperature in O. fragrans. RESULTS: The cell size of adaxial and abaxial petal epidermal cells and ultrastructural morphology of petal cells at different developmental stages were observed. The cell size of adaxial and abaxial petal epidermal cells increased gradually with the process of flower opening. Then the transcriptomic sequencing was employed to analyze the differentially expressed genes (DEGs) under different number of days’ treatments with relatively low temperatures (19 °C) or 23 °C. Analysis of DEGs in Gene Ontology analysis showed that “metabolic process”, “cellular process”, “binding”, “catalytic activity”, “cell”, “cell part”, “membrane”, “membrane part”, “single-organism process”, and “organelle” were highly enriched. In KEGG analysis, “metabolic pathways”, “biosynthesis of secondary metabolites”, “plant-pathogen interaction”, “starch and sucrose metabolism”, and “plant hormone signal transduction” were the top five pathways containing the greatest number of DEGs. The DEGs involved in cell wall metabolism, phytohormone signal transduction pathways, and eight kinds of transcription factors were analyzed in depth. CONCLUSIONS: Several unigenes involved in cell wall metabolism, phytohormone signal transduction pathway, and transcription factors with highly variable expression levels between different temperature treatments may be involved in petal cell expansion during flower opening process in response to the relatively low temperature. These results could improve our understanding of the molecular mechanism of relatively-low-temperature-regulated flower opening of O. fragrans, provide practical information for the prediction and regulation of flowering time in O. fragrans, and ultimately pave the way for genetic modification in O. fragrans.
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spelling pubmed-73674002020-07-20 Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans Fu, Jianxin Zhang, Chao Liu, Yucheng Pang, Tianhong Dong, Bin Gao, Xiaoyue Zhu, Yimin Zhao, Hongbo BMC Plant Biol Research Article BACKGROUND: Sweet osmanthus (Osmanthus fragrans Lour.) is one of the top ten traditional ornamental flowers in China. The flowering time of once-flowering cultivars in O. fragrans is greatly affected by the relatively low temperature, but there are few reports on its molecular mechanism to date. A hypothesis had been raised that genes related with flower opening might be up-regulated in response to relatively low temperature in O. fragrans. Thus, our work was aimed to explore the underlying molecular mechanism of flower opening regulated by relatively low temperature in O. fragrans. RESULTS: The cell size of adaxial and abaxial petal epidermal cells and ultrastructural morphology of petal cells at different developmental stages were observed. The cell size of adaxial and abaxial petal epidermal cells increased gradually with the process of flower opening. Then the transcriptomic sequencing was employed to analyze the differentially expressed genes (DEGs) under different number of days’ treatments with relatively low temperatures (19 °C) or 23 °C. Analysis of DEGs in Gene Ontology analysis showed that “metabolic process”, “cellular process”, “binding”, “catalytic activity”, “cell”, “cell part”, “membrane”, “membrane part”, “single-organism process”, and “organelle” were highly enriched. In KEGG analysis, “metabolic pathways”, “biosynthesis of secondary metabolites”, “plant-pathogen interaction”, “starch and sucrose metabolism”, and “plant hormone signal transduction” were the top five pathways containing the greatest number of DEGs. The DEGs involved in cell wall metabolism, phytohormone signal transduction pathways, and eight kinds of transcription factors were analyzed in depth. CONCLUSIONS: Several unigenes involved in cell wall metabolism, phytohormone signal transduction pathway, and transcription factors with highly variable expression levels between different temperature treatments may be involved in petal cell expansion during flower opening process in response to the relatively low temperature. These results could improve our understanding of the molecular mechanism of relatively-low-temperature-regulated flower opening of O. fragrans, provide practical information for the prediction and regulation of flowering time in O. fragrans, and ultimately pave the way for genetic modification in O. fragrans. BioMed Central 2020-07-16 /pmc/articles/PMC7367400/ /pubmed/32677959 http://dx.doi.org/10.1186/s12870-020-02549-3 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Fu, Jianxin
Zhang, Chao
Liu, Yucheng
Pang, Tianhong
Dong, Bin
Gao, Xiaoyue
Zhu, Yimin
Zhao, Hongbo
Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans
title Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans
title_full Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans
title_fullStr Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans
title_full_unstemmed Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans
title_short Transcriptomic analysis of flower opening response to relatively low temperatures in Osmanthus fragrans
title_sort transcriptomic analysis of flower opening response to relatively low temperatures in osmanthus fragrans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367400/
https://www.ncbi.nlm.nih.gov/pubmed/32677959
http://dx.doi.org/10.1186/s12870-020-02549-3
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