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Variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean

Rhizobia–legume symbiosis is an important type of plant–microbe mutualism; however, the establishment of this association is complicated and can be affected by many factors. The soybean rhizosphere has a specific microbial community, yet whether these organisms affect rhizobial nodulation has not be...

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Autores principales: Han, Qin, Ma, Qun, Chen, Yong, Tian, Bing, Xu, Lanxi, Bai, Yang, Chen, Wenfeng, Li, Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367843/
https://www.ncbi.nlm.nih.gov/pubmed/32336748
http://dx.doi.org/10.1038/s41396-020-0648-9
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author Han, Qin
Ma, Qun
Chen, Yong
Tian, Bing
Xu, Lanxi
Bai, Yang
Chen, Wenfeng
Li, Xia
author_facet Han, Qin
Ma, Qun
Chen, Yong
Tian, Bing
Xu, Lanxi
Bai, Yang
Chen, Wenfeng
Li, Xia
author_sort Han, Qin
collection PubMed
description Rhizobia–legume symbiosis is an important type of plant–microbe mutualism; however, the establishment of this association is complicated and can be affected by many factors. The soybean rhizosphere has a specific microbial community, yet whether these organisms affect rhizobial nodulation has not been well investigated. Here, we analyzed the compositions and relationships of soybean rhizocompartment microbiota in three types of soil. First, we found that the rhizosphere community composition of soybean varied significantly in different soils, and the association network between rhizobia and other rhizosphere bacteria was examined. Second, we found that some rhizosphere microbes were correlated with the composition of bradyrhizobia and sinorhizobia in nodules. We cultivated 278 candidate Bacillus isolates from alkaline soil. Finally, interaction and nodulation assays showed that the Bacillus cereus group specifically promotes and suppresses the growth of sinorhizobia and bradyrhizobia, respectively, and alleviates the effects of saline–alkali conditions on the nodulation of sinorhizobia as well as affecting its colonization in nodules. Our findings demonstrate a crucial role of the bacterial microbiota in shaping rhizobia–host interactions in soybean, and provide a framework for improving the symbiotic efficiency of this system of mutualism through the use of synthetic bacterial communities.
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spelling pubmed-73678432020-07-21 Variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean Han, Qin Ma, Qun Chen, Yong Tian, Bing Xu, Lanxi Bai, Yang Chen, Wenfeng Li, Xia ISME J Article Rhizobia–legume symbiosis is an important type of plant–microbe mutualism; however, the establishment of this association is complicated and can be affected by many factors. The soybean rhizosphere has a specific microbial community, yet whether these organisms affect rhizobial nodulation has not been well investigated. Here, we analyzed the compositions and relationships of soybean rhizocompartment microbiota in three types of soil. First, we found that the rhizosphere community composition of soybean varied significantly in different soils, and the association network between rhizobia and other rhizosphere bacteria was examined. Second, we found that some rhizosphere microbes were correlated with the composition of bradyrhizobia and sinorhizobia in nodules. We cultivated 278 candidate Bacillus isolates from alkaline soil. Finally, interaction and nodulation assays showed that the Bacillus cereus group specifically promotes and suppresses the growth of sinorhizobia and bradyrhizobia, respectively, and alleviates the effects of saline–alkali conditions on the nodulation of sinorhizobia as well as affecting its colonization in nodules. Our findings demonstrate a crucial role of the bacterial microbiota in shaping rhizobia–host interactions in soybean, and provide a framework for improving the symbiotic efficiency of this system of mutualism through the use of synthetic bacterial communities. Nature Publishing Group UK 2020-04-27 2020-08 /pmc/articles/PMC7367843/ /pubmed/32336748 http://dx.doi.org/10.1038/s41396-020-0648-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Han, Qin
Ma, Qun
Chen, Yong
Tian, Bing
Xu, Lanxi
Bai, Yang
Chen, Wenfeng
Li, Xia
Variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean
title Variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean
title_full Variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean
title_fullStr Variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean
title_full_unstemmed Variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean
title_short Variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean
title_sort variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367843/
https://www.ncbi.nlm.nih.gov/pubmed/32336748
http://dx.doi.org/10.1038/s41396-020-0648-9
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