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A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells
Coat protein complex I (COPI)–coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368096/ https://www.ncbi.nlm.nih.gov/pubmed/32665377 http://dx.doi.org/10.26508/lsa.202000714 |
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author | Jain Goyal, Manu Zhao, Xiyan Bozhinova, Mariya Andrade-López, Karla de Heus, Cecilia Schulze-Dramac, Sandra Müller-McNicoll, Michaela Klumperman, Judith Béthune, Julien |
author_facet | Jain Goyal, Manu Zhao, Xiyan Bozhinova, Mariya Andrade-López, Karla de Heus, Cecilia Schulze-Dramac, Sandra Müller-McNicoll, Michaela Klumperman, Judith Béthune, Julien |
author_sort | Jain Goyal, Manu |
collection | PubMed |
description | Coat protein complex I (COPI)–coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether paralogous COPI proteins have specific functions is currently unknown. Here, we show that the paralogous coatomer subunits γ1-COP and γ2-COP are differentially expressed during the neuronal differentiation of mouse pluripotent cells. Moreover, through a combination of genome editing experiments, we demonstrate that whereas γ-COP paralogs are largely functionally redundant, γ1-COP specifically promotes neurite outgrowth. Our work stresses a role of the COPI pathway in neuronal polarization and provides evidence for distinct functions for coatomer paralogous subunits in this process. |
format | Online Article Text |
id | pubmed-7368096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-73680962020-07-31 A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells Jain Goyal, Manu Zhao, Xiyan Bozhinova, Mariya Andrade-López, Karla de Heus, Cecilia Schulze-Dramac, Sandra Müller-McNicoll, Michaela Klumperman, Judith Béthune, Julien Life Sci Alliance Research Articles Coat protein complex I (COPI)–coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether paralogous COPI proteins have specific functions is currently unknown. Here, we show that the paralogous coatomer subunits γ1-COP and γ2-COP are differentially expressed during the neuronal differentiation of mouse pluripotent cells. Moreover, through a combination of genome editing experiments, we demonstrate that whereas γ-COP paralogs are largely functionally redundant, γ1-COP specifically promotes neurite outgrowth. Our work stresses a role of the COPI pathway in neuronal polarization and provides evidence for distinct functions for coatomer paralogous subunits in this process. Life Science Alliance LLC 2020-07-14 /pmc/articles/PMC7368096/ /pubmed/32665377 http://dx.doi.org/10.26508/lsa.202000714 Text en © 2020 Goyal et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Jain Goyal, Manu Zhao, Xiyan Bozhinova, Mariya Andrade-López, Karla de Heus, Cecilia Schulze-Dramac, Sandra Müller-McNicoll, Michaela Klumperman, Judith Béthune, Julien A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells |
title | A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells |
title_full | A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells |
title_fullStr | A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells |
title_full_unstemmed | A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells |
title_short | A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells |
title_sort | paralog-specific role of copi vesicles in the neuronal differentiation of mouse pluripotent cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368096/ https://www.ncbi.nlm.nih.gov/pubmed/32665377 http://dx.doi.org/10.26508/lsa.202000714 |
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