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A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells

Coat protein complex I (COPI)–coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether...

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Autores principales: Jain Goyal, Manu, Zhao, Xiyan, Bozhinova, Mariya, Andrade-López, Karla, de Heus, Cecilia, Schulze-Dramac, Sandra, Müller-McNicoll, Michaela, Klumperman, Judith, Béthune, Julien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Life Science Alliance LLC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368096/
https://www.ncbi.nlm.nih.gov/pubmed/32665377
http://dx.doi.org/10.26508/lsa.202000714
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author Jain Goyal, Manu
Zhao, Xiyan
Bozhinova, Mariya
Andrade-López, Karla
de Heus, Cecilia
Schulze-Dramac, Sandra
Müller-McNicoll, Michaela
Klumperman, Judith
Béthune, Julien
author_facet Jain Goyal, Manu
Zhao, Xiyan
Bozhinova, Mariya
Andrade-López, Karla
de Heus, Cecilia
Schulze-Dramac, Sandra
Müller-McNicoll, Michaela
Klumperman, Judith
Béthune, Julien
author_sort Jain Goyal, Manu
collection PubMed
description Coat protein complex I (COPI)–coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether paralogous COPI proteins have specific functions is currently unknown. Here, we show that the paralogous coatomer subunits γ1-COP and γ2-COP are differentially expressed during the neuronal differentiation of mouse pluripotent cells. Moreover, through a combination of genome editing experiments, we demonstrate that whereas γ-COP paralogs are largely functionally redundant, γ1-COP specifically promotes neurite outgrowth. Our work stresses a role of the COPI pathway in neuronal polarization and provides evidence for distinct functions for coatomer paralogous subunits in this process.
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spelling pubmed-73680962020-07-31 A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells Jain Goyal, Manu Zhao, Xiyan Bozhinova, Mariya Andrade-López, Karla de Heus, Cecilia Schulze-Dramac, Sandra Müller-McNicoll, Michaela Klumperman, Judith Béthune, Julien Life Sci Alliance Research Articles Coat protein complex I (COPI)–coated vesicles mediate membrane trafficking between Golgi cisternae as well as retrieval of proteins from the Golgi to the endoplasmic reticulum. There are several flavors of the COPI coat defined by paralogous subunits of the protein complex coatomer. However, whether paralogous COPI proteins have specific functions is currently unknown. Here, we show that the paralogous coatomer subunits γ1-COP and γ2-COP are differentially expressed during the neuronal differentiation of mouse pluripotent cells. Moreover, through a combination of genome editing experiments, we demonstrate that whereas γ-COP paralogs are largely functionally redundant, γ1-COP specifically promotes neurite outgrowth. Our work stresses a role of the COPI pathway in neuronal polarization and provides evidence for distinct functions for coatomer paralogous subunits in this process. Life Science Alliance LLC 2020-07-14 /pmc/articles/PMC7368096/ /pubmed/32665377 http://dx.doi.org/10.26508/lsa.202000714 Text en © 2020 Goyal et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Jain Goyal, Manu
Zhao, Xiyan
Bozhinova, Mariya
Andrade-López, Karla
de Heus, Cecilia
Schulze-Dramac, Sandra
Müller-McNicoll, Michaela
Klumperman, Judith
Béthune, Julien
A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells
title A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells
title_full A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells
title_fullStr A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells
title_full_unstemmed A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells
title_short A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells
title_sort paralog-specific role of copi vesicles in the neuronal differentiation of mouse pluripotent cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368096/
https://www.ncbi.nlm.nih.gov/pubmed/32665377
http://dx.doi.org/10.26508/lsa.202000714
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