Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)

BACKGROUND: Porcine circovirus type 3 (PCV3) has been an emerging porcine virus spread around the world. The conserved DNA sequence of PCV3 enabled good performance in molecular biological assays. RESULT: In this study, we developed a real-time fluorescence PCR assay for the detection of PCV3. The c...

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Autores principales: Yuan, Lin, Liu, Yingyi, Chen, Yana, Gu, Xiaoxue, Dong, Hao, Zhang, Shuo, Han, Tao, Zhou, Zhi, Song, Xiaohui, Wang, Chuanbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368764/
https://www.ncbi.nlm.nih.gov/pubmed/32680512
http://dx.doi.org/10.1186/s12917-020-02435-y
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author Yuan, Lin
Liu, Yingyi
Chen, Yana
Gu, Xiaoxue
Dong, Hao
Zhang, Shuo
Han, Tao
Zhou, Zhi
Song, Xiaohui
Wang, Chuanbin
author_facet Yuan, Lin
Liu, Yingyi
Chen, Yana
Gu, Xiaoxue
Dong, Hao
Zhang, Shuo
Han, Tao
Zhou, Zhi
Song, Xiaohui
Wang, Chuanbin
author_sort Yuan, Lin
collection PubMed
description BACKGROUND: Porcine circovirus type 3 (PCV3) has been an emerging porcine virus spread around the world. The conserved DNA sequence of PCV3 enabled good performance in molecular biological assays. RESULT: In this study, we developed a real-time fluorescence PCR assay for the detection of PCV3. The conserved region within Capsid genome of PCV3 was selected for the design of primer pairs and probes. After optimizing, a primer pair and probe was screened, providing high sensitivity (10 copies/μL) and specificity (no cross reaction with other porcine viruses or common bacterium). In addition, this method was applied in the detection of 110 clinical samples, and the performance was compared with other previously reported PCR and real-time PCR methods. This method provided higher detection rate. CONCLUSION: A real-time fluorescence PCR assay has been developed for the detection of PCV3, with high sensitivity and specificity, exhibiting good performance in detecting clinical samples.
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spelling pubmed-73687642020-07-20 Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3) Yuan, Lin Liu, Yingyi Chen, Yana Gu, Xiaoxue Dong, Hao Zhang, Shuo Han, Tao Zhou, Zhi Song, Xiaohui Wang, Chuanbin BMC Vet Res Methodology Article BACKGROUND: Porcine circovirus type 3 (PCV3) has been an emerging porcine virus spread around the world. The conserved DNA sequence of PCV3 enabled good performance in molecular biological assays. RESULT: In this study, we developed a real-time fluorescence PCR assay for the detection of PCV3. The conserved region within Capsid genome of PCV3 was selected for the design of primer pairs and probes. After optimizing, a primer pair and probe was screened, providing high sensitivity (10 copies/μL) and specificity (no cross reaction with other porcine viruses or common bacterium). In addition, this method was applied in the detection of 110 clinical samples, and the performance was compared with other previously reported PCR and real-time PCR methods. This method provided higher detection rate. CONCLUSION: A real-time fluorescence PCR assay has been developed for the detection of PCV3, with high sensitivity and specificity, exhibiting good performance in detecting clinical samples. BioMed Central 2020-07-17 /pmc/articles/PMC7368764/ /pubmed/32680512 http://dx.doi.org/10.1186/s12917-020-02435-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Methodology Article
Yuan, Lin
Liu, Yingyi
Chen, Yana
Gu, Xiaoxue
Dong, Hao
Zhang, Shuo
Han, Tao
Zhou, Zhi
Song, Xiaohui
Wang, Chuanbin
Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)
title Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)
title_full Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)
title_fullStr Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)
title_full_unstemmed Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)
title_short Optimized real-time fluorescence PCR assay for the detection of porcine Circovirus type 3 (PCV3)
title_sort optimized real-time fluorescence pcr assay for the detection of porcine circovirus type 3 (pcv3)
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368764/
https://www.ncbi.nlm.nih.gov/pubmed/32680512
http://dx.doi.org/10.1186/s12917-020-02435-y
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