Cargando…

An Image Analysis Solution For Quantification and Determination of Immunohistochemistry Staining Reproducibility

With immunohistochemical (IHC) staining increasingly being used to guide clinical decisions, variability in staining quality and reproducibility are becoming essential factors in generating diagnoses using IHC tissue preparations. The current study tested a method to track and quantify the interrun,...

Descripción completa

Detalles Bibliográficos
Autores principales: Chlipala, Elizabeth A., Bendzinski, Christine M., Dorner, Charlie, Sartan, Raili, Copeland, Karen, Pearce, Roger, Doherty, Faye, Bolon, Brad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368846/
https://www.ncbi.nlm.nih.gov/pubmed/31082827
http://dx.doi.org/10.1097/PAI.0000000000000776
_version_ 1783560672117784576
author Chlipala, Elizabeth A.
Bendzinski, Christine M.
Dorner, Charlie
Sartan, Raili
Copeland, Karen
Pearce, Roger
Doherty, Faye
Bolon, Brad
author_facet Chlipala, Elizabeth A.
Bendzinski, Christine M.
Dorner, Charlie
Sartan, Raili
Copeland, Karen
Pearce, Roger
Doherty, Faye
Bolon, Brad
author_sort Chlipala, Elizabeth A.
collection PubMed
description With immunohistochemical (IHC) staining increasingly being used to guide clinical decisions, variability in staining quality and reproducibility are becoming essential factors in generating diagnoses using IHC tissue preparations. The current study tested a method to track and quantify the interrun, intrarun, and intersite variability of IHC staining intensity. Our hypothesis was that staining precision between laboratory sites, staining runs, and individual slides may be verified quantitatively, efficiently and effectively utilizing algorithm-based, automated image analysis. To investigate this premise, we tested the consistency of IHC staining in 40 routinely processed (formalin-fixed, paraffin-embedded) human tissues using 10 common antibiomarker antibodies on 2 Dako Omnis instruments at 2 locations (Carpinteria, CA: 30 m above sea level and Longmont, CO: 1500 m above sea level) programmed with identical, default settings and sample pretreatments. Digital images of IHC-labeled sections produced by a whole slide scanner were analyzed by a simple commercially available algorithm and compared with a board-certified veterinary pathologist’s semiquantitative scoring of staining intensity. The image analysis output correlated well with pathology scores but had increased sensitivity for discriminating subtle variations and providing reproducible digital quantification across sites as well as within and among staining runs at the same site. Taken together, our data indicate that digital image analysis offers an objective and quantifiable means of verifying IHC staining parameters as a part of laboratory quality assurance systems.
format Online
Article
Text
id pubmed-7368846
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Lippincott Williams & Wilkins
record_format MEDLINE/PubMed
spelling pubmed-73688462020-08-05 An Image Analysis Solution For Quantification and Determination of Immunohistochemistry Staining Reproducibility Chlipala, Elizabeth A. Bendzinski, Christine M. Dorner, Charlie Sartan, Raili Copeland, Karen Pearce, Roger Doherty, Faye Bolon, Brad Appl Immunohistochem Mol Morphol Research Articles With immunohistochemical (IHC) staining increasingly being used to guide clinical decisions, variability in staining quality and reproducibility are becoming essential factors in generating diagnoses using IHC tissue preparations. The current study tested a method to track and quantify the interrun, intrarun, and intersite variability of IHC staining intensity. Our hypothesis was that staining precision between laboratory sites, staining runs, and individual slides may be verified quantitatively, efficiently and effectively utilizing algorithm-based, automated image analysis. To investigate this premise, we tested the consistency of IHC staining in 40 routinely processed (formalin-fixed, paraffin-embedded) human tissues using 10 common antibiomarker antibodies on 2 Dako Omnis instruments at 2 locations (Carpinteria, CA: 30 m above sea level and Longmont, CO: 1500 m above sea level) programmed with identical, default settings and sample pretreatments. Digital images of IHC-labeled sections produced by a whole slide scanner were analyzed by a simple commercially available algorithm and compared with a board-certified veterinary pathologist’s semiquantitative scoring of staining intensity. The image analysis output correlated well with pathology scores but had increased sensitivity for discriminating subtle variations and providing reproducible digital quantification across sites as well as within and among staining runs at the same site. Taken together, our data indicate that digital image analysis offers an objective and quantifiable means of verifying IHC staining parameters as a part of laboratory quality assurance systems. Lippincott Williams & Wilkins 2020-07 2019-05-06 /pmc/articles/PMC7368846/ /pubmed/31082827 http://dx.doi.org/10.1097/PAI.0000000000000776 Text en Copyright © 2019 The Author(s). Published by Wolters Kluwer Health, Inc. This is an open access article distributed under the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0/) (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Articles
Chlipala, Elizabeth A.
Bendzinski, Christine M.
Dorner, Charlie
Sartan, Raili
Copeland, Karen
Pearce, Roger
Doherty, Faye
Bolon, Brad
An Image Analysis Solution For Quantification and Determination of Immunohistochemistry Staining Reproducibility
title An Image Analysis Solution For Quantification and Determination of Immunohistochemistry Staining Reproducibility
title_full An Image Analysis Solution For Quantification and Determination of Immunohistochemistry Staining Reproducibility
title_fullStr An Image Analysis Solution For Quantification and Determination of Immunohistochemistry Staining Reproducibility
title_full_unstemmed An Image Analysis Solution For Quantification and Determination of Immunohistochemistry Staining Reproducibility
title_short An Image Analysis Solution For Quantification and Determination of Immunohistochemistry Staining Reproducibility
title_sort image analysis solution for quantification and determination of immunohistochemistry staining reproducibility
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7368846/
https://www.ncbi.nlm.nih.gov/pubmed/31082827
http://dx.doi.org/10.1097/PAI.0000000000000776
work_keys_str_mv AT chlipalaelizabetha animageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT bendzinskichristinem animageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT dornercharlie animageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT sartanraili animageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT copelandkaren animageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT pearceroger animageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT dohertyfaye animageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT bolonbrad animageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT chlipalaelizabetha imageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT bendzinskichristinem imageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT dornercharlie imageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT sartanraili imageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT copelandkaren imageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT pearceroger imageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT dohertyfaye imageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility
AT bolonbrad imageanalysissolutionforquantificationanddeterminationofimmunohistochemistrystainingreproducibility