Cargando…

Metabolic Characterisation of the Midgut of Bombyx mori Varieties after BmNPV Infection Using GC-MS-Based Metabolite Profiling

Bombyx mori nucleopolyhedrovirus (BmNPV) is a silkworm disease that is especially harmful to cocoon production and seriously restricts sericultural development. Our laboratory successfully cultivated a new highly BmNPV-resistant silkworm variety, Huakang 2; however, its mechanism of BmNPV resistance...

Descripción completa

Detalles Bibliográficos
Autores principales: Qian, Heying, Li, Gang, Zhao, Guodong, Liu, Mingzhu, Xu, Anying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7369710/
https://www.ncbi.nlm.nih.gov/pubmed/32630275
http://dx.doi.org/10.3390/ijms21134707
_version_ 1783560830800887808
author Qian, Heying
Li, Gang
Zhao, Guodong
Liu, Mingzhu
Xu, Anying
author_facet Qian, Heying
Li, Gang
Zhao, Guodong
Liu, Mingzhu
Xu, Anying
author_sort Qian, Heying
collection PubMed
description Bombyx mori nucleopolyhedrovirus (BmNPV) is a silkworm disease that is especially harmful to cocoon production and seriously restricts sericultural development. Our laboratory successfully cultivated a new highly BmNPV-resistant silkworm variety, Huakang 2; however, its mechanism of BmNPV resistance remains unclear. To understand its resistance mechanism, we conducted a metabolomic and transcriptomic study of the midgut of silkworm varieties, Baiyu N and Baiyu after BmNPV infection. We identified 451 differential metabolites, which were mostly comprised of small molecules, such as saccharides, acids, amines, alcohols, and glycosides. We found that the primary differences in disease resistance between the silkworm varieties are metabolic-pathways, tryptophan metabolism, oxidative phosphorylation, ABC-transporters, beta-alanine metabolism, and phenylalanine metabolism. Combined analysis with transcriptomic data suggested that tryptophan metabolism and oxidative phosphorylation are closely related to the silkworms’ BmNPV resistance. We hypothesize that the roles of the two metabolic pathways in the BmNPV resistance mechanism might be the following: Oxidative phosphorylation generates a large amount of adenosine triphosphate (ATP) in response to BmNPV infection to provide silkworms the energy required for establishing BmNPV resistance. Tryptophan metabolism then activates the aryl hydrocarbon receptor (AhR) through the exogenous virus BmNPV, which activates the silkworm’s immune system to defeat BmNPV infections.
format Online
Article
Text
id pubmed-7369710
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73697102020-07-21 Metabolic Characterisation of the Midgut of Bombyx mori Varieties after BmNPV Infection Using GC-MS-Based Metabolite Profiling Qian, Heying Li, Gang Zhao, Guodong Liu, Mingzhu Xu, Anying Int J Mol Sci Article Bombyx mori nucleopolyhedrovirus (BmNPV) is a silkworm disease that is especially harmful to cocoon production and seriously restricts sericultural development. Our laboratory successfully cultivated a new highly BmNPV-resistant silkworm variety, Huakang 2; however, its mechanism of BmNPV resistance remains unclear. To understand its resistance mechanism, we conducted a metabolomic and transcriptomic study of the midgut of silkworm varieties, Baiyu N and Baiyu after BmNPV infection. We identified 451 differential metabolites, which were mostly comprised of small molecules, such as saccharides, acids, amines, alcohols, and glycosides. We found that the primary differences in disease resistance between the silkworm varieties are metabolic-pathways, tryptophan metabolism, oxidative phosphorylation, ABC-transporters, beta-alanine metabolism, and phenylalanine metabolism. Combined analysis with transcriptomic data suggested that tryptophan metabolism and oxidative phosphorylation are closely related to the silkworms’ BmNPV resistance. We hypothesize that the roles of the two metabolic pathways in the BmNPV resistance mechanism might be the following: Oxidative phosphorylation generates a large amount of adenosine triphosphate (ATP) in response to BmNPV infection to provide silkworms the energy required for establishing BmNPV resistance. Tryptophan metabolism then activates the aryl hydrocarbon receptor (AhR) through the exogenous virus BmNPV, which activates the silkworm’s immune system to defeat BmNPV infections. MDPI 2020-07-01 /pmc/articles/PMC7369710/ /pubmed/32630275 http://dx.doi.org/10.3390/ijms21134707 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qian, Heying
Li, Gang
Zhao, Guodong
Liu, Mingzhu
Xu, Anying
Metabolic Characterisation of the Midgut of Bombyx mori Varieties after BmNPV Infection Using GC-MS-Based Metabolite Profiling
title Metabolic Characterisation of the Midgut of Bombyx mori Varieties after BmNPV Infection Using GC-MS-Based Metabolite Profiling
title_full Metabolic Characterisation of the Midgut of Bombyx mori Varieties after BmNPV Infection Using GC-MS-Based Metabolite Profiling
title_fullStr Metabolic Characterisation of the Midgut of Bombyx mori Varieties after BmNPV Infection Using GC-MS-Based Metabolite Profiling
title_full_unstemmed Metabolic Characterisation of the Midgut of Bombyx mori Varieties after BmNPV Infection Using GC-MS-Based Metabolite Profiling
title_short Metabolic Characterisation of the Midgut of Bombyx mori Varieties after BmNPV Infection Using GC-MS-Based Metabolite Profiling
title_sort metabolic characterisation of the midgut of bombyx mori varieties after bmnpv infection using gc-ms-based metabolite profiling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7369710/
https://www.ncbi.nlm.nih.gov/pubmed/32630275
http://dx.doi.org/10.3390/ijms21134707
work_keys_str_mv AT qianheying metaboliccharacterisationofthemidgutofbombyxmorivarietiesafterbmnpvinfectionusinggcmsbasedmetaboliteprofiling
AT ligang metaboliccharacterisationofthemidgutofbombyxmorivarietiesafterbmnpvinfectionusinggcmsbasedmetaboliteprofiling
AT zhaoguodong metaboliccharacterisationofthemidgutofbombyxmorivarietiesafterbmnpvinfectionusinggcmsbasedmetaboliteprofiling
AT liumingzhu metaboliccharacterisationofthemidgutofbombyxmorivarietiesafterbmnpvinfectionusinggcmsbasedmetaboliteprofiling
AT xuanying metaboliccharacterisationofthemidgutofbombyxmorivarietiesafterbmnpvinfectionusinggcmsbasedmetaboliteprofiling