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The Impact of Single-Cell Genomics on Adipose Tissue Research

Adipose tissue is an important regulator of whole-body metabolism and energy homeostasis. The unprecedented growth of obesity and metabolic disease worldwide has required paralleled advancements in research on this dynamic endocrine organ system. Single-cell RNA sequencing (scRNA-seq), a highly meti...

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Detalles Bibliográficos
Autores principales: Deutsch, Alana, Feng, Daorong, Pessin, Jeffrey E., Shinoda, Kosaku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7369959/
https://www.ncbi.nlm.nih.gov/pubmed/32635651
http://dx.doi.org/10.3390/ijms21134773
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author Deutsch, Alana
Feng, Daorong
Pessin, Jeffrey E.
Shinoda, Kosaku
author_facet Deutsch, Alana
Feng, Daorong
Pessin, Jeffrey E.
Shinoda, Kosaku
author_sort Deutsch, Alana
collection PubMed
description Adipose tissue is an important regulator of whole-body metabolism and energy homeostasis. The unprecedented growth of obesity and metabolic disease worldwide has required paralleled advancements in research on this dynamic endocrine organ system. Single-cell RNA sequencing (scRNA-seq), a highly meticulous methodology used to dissect tissue heterogeneity through the transcriptional characterization of individual cells, is responsible for facilitating critical advancements in this area. The unique investigative capabilities achieved by the combination of nanotechnology, molecular biology, and informatics are expanding our understanding of adipose tissue’s composition and compartmentalized functional specialization, which underlie physiologic and pathogenic states, including adaptive thermogenesis, adipose tissue aging, and obesity. In this review, we will summarize the use of scRNA-seq and single-nuclei RNA-seq (snRNA-seq) in adipocyte biology and their applications to obesity and diabetes research in the hopes of increasing awareness of the capabilities of this technology and acting as a catalyst for its expanded use in further investigation.
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spelling pubmed-73699592020-07-21 The Impact of Single-Cell Genomics on Adipose Tissue Research Deutsch, Alana Feng, Daorong Pessin, Jeffrey E. Shinoda, Kosaku Int J Mol Sci Review Adipose tissue is an important regulator of whole-body metabolism and energy homeostasis. The unprecedented growth of obesity and metabolic disease worldwide has required paralleled advancements in research on this dynamic endocrine organ system. Single-cell RNA sequencing (scRNA-seq), a highly meticulous methodology used to dissect tissue heterogeneity through the transcriptional characterization of individual cells, is responsible for facilitating critical advancements in this area. The unique investigative capabilities achieved by the combination of nanotechnology, molecular biology, and informatics are expanding our understanding of adipose tissue’s composition and compartmentalized functional specialization, which underlie physiologic and pathogenic states, including adaptive thermogenesis, adipose tissue aging, and obesity. In this review, we will summarize the use of scRNA-seq and single-nuclei RNA-seq (snRNA-seq) in adipocyte biology and their applications to obesity and diabetes research in the hopes of increasing awareness of the capabilities of this technology and acting as a catalyst for its expanded use in further investigation. MDPI 2020-07-05 /pmc/articles/PMC7369959/ /pubmed/32635651 http://dx.doi.org/10.3390/ijms21134773 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Deutsch, Alana
Feng, Daorong
Pessin, Jeffrey E.
Shinoda, Kosaku
The Impact of Single-Cell Genomics on Adipose Tissue Research
title The Impact of Single-Cell Genomics on Adipose Tissue Research
title_full The Impact of Single-Cell Genomics on Adipose Tissue Research
title_fullStr The Impact of Single-Cell Genomics on Adipose Tissue Research
title_full_unstemmed The Impact of Single-Cell Genomics on Adipose Tissue Research
title_short The Impact of Single-Cell Genomics on Adipose Tissue Research
title_sort impact of single-cell genomics on adipose tissue research
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7369959/
https://www.ncbi.nlm.nih.gov/pubmed/32635651
http://dx.doi.org/10.3390/ijms21134773
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