Cargando…
Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury
Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal i...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370182/ https://www.ncbi.nlm.nih.gov/pubmed/32635660 http://dx.doi.org/10.3390/ijms21134774 |
_version_ | 1783560940550094848 |
---|---|
author | Lee, Jun Ho Ha, Dae Hyun Go, Hyeon-kyu Youn, Jinkwon Kim, Hyun-keun Jin, Richard C. Miller, Randy B. Kim, Do-hyung Cho, Byong Seung Yi, Yong Weon |
author_facet | Lee, Jun Ho Ha, Dae Hyun Go, Hyeon-kyu Youn, Jinkwon Kim, Hyun-keun Jin, Richard C. Miller, Randy B. Kim, Do-hyung Cho, Byong Seung Yi, Yong Weon |
author_sort | Lee, Jun Ho |
collection | PubMed |
description | Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal injuries in multiple experimental animal models of kidney diseases including AKI. However, the mass production of exosomes is a challenge not only in preclinical studies with large animals but also for successful clinical applications. In this respect, tangential flow filtration (TFF) is suitable for good manufacturing practice (GMP)-compliant large-scale production of high-quality exosomes. Until now, no studies have been reported on the use of TFF, but rather ultracentrifugation has been almost exclusively used, to isolate exosomes for AKI therapeutic application in preclinical studies. Here, we demonstrated the reproducible large-scale production of exosomes derived from adipose tissue-derived MSC (ASC-exosomes) using TFF and the lifesaving effect of the ASC-exosomes in a lethal model of cisplatin-induced rat AKI. Our results suggest the possibility of large-scale stable production of ASC-exosomes without loss of function and their successful application in life-threatening diseases. |
format | Online Article Text |
id | pubmed-7370182 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73701822020-07-21 Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury Lee, Jun Ho Ha, Dae Hyun Go, Hyeon-kyu Youn, Jinkwon Kim, Hyun-keun Jin, Richard C. Miller, Randy B. Kim, Do-hyung Cho, Byong Seung Yi, Yong Weon Int J Mol Sci Article Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal injuries in multiple experimental animal models of kidney diseases including AKI. However, the mass production of exosomes is a challenge not only in preclinical studies with large animals but also for successful clinical applications. In this respect, tangential flow filtration (TFF) is suitable for good manufacturing practice (GMP)-compliant large-scale production of high-quality exosomes. Until now, no studies have been reported on the use of TFF, but rather ultracentrifugation has been almost exclusively used, to isolate exosomes for AKI therapeutic application in preclinical studies. Here, we demonstrated the reproducible large-scale production of exosomes derived from adipose tissue-derived MSC (ASC-exosomes) using TFF and the lifesaving effect of the ASC-exosomes in a lethal model of cisplatin-induced rat AKI. Our results suggest the possibility of large-scale stable production of ASC-exosomes without loss of function and their successful application in life-threatening diseases. MDPI 2020-07-05 /pmc/articles/PMC7370182/ /pubmed/32635660 http://dx.doi.org/10.3390/ijms21134774 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Jun Ho Ha, Dae Hyun Go, Hyeon-kyu Youn, Jinkwon Kim, Hyun-keun Jin, Richard C. Miller, Randy B. Kim, Do-hyung Cho, Byong Seung Yi, Yong Weon Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury |
title | Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury |
title_full | Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury |
title_fullStr | Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury |
title_full_unstemmed | Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury |
title_short | Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury |
title_sort | reproducible large-scale isolation of exosomes from adipose tissue-derived mesenchymal stem/stromal cells and their application in acute kidney injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370182/ https://www.ncbi.nlm.nih.gov/pubmed/32635660 http://dx.doi.org/10.3390/ijms21134774 |
work_keys_str_mv | AT leejunho reproduciblelargescaleisolationofexosomesfromadiposetissuederivedmesenchymalstemstromalcellsandtheirapplicationinacutekidneyinjury AT hadaehyun reproduciblelargescaleisolationofexosomesfromadiposetissuederivedmesenchymalstemstromalcellsandtheirapplicationinacutekidneyinjury AT gohyeonkyu reproduciblelargescaleisolationofexosomesfromadiposetissuederivedmesenchymalstemstromalcellsandtheirapplicationinacutekidneyinjury AT younjinkwon reproduciblelargescaleisolationofexosomesfromadiposetissuederivedmesenchymalstemstromalcellsandtheirapplicationinacutekidneyinjury AT kimhyunkeun reproduciblelargescaleisolationofexosomesfromadiposetissuederivedmesenchymalstemstromalcellsandtheirapplicationinacutekidneyinjury AT jinrichardc reproduciblelargescaleisolationofexosomesfromadiposetissuederivedmesenchymalstemstromalcellsandtheirapplicationinacutekidneyinjury AT millerrandyb reproduciblelargescaleisolationofexosomesfromadiposetissuederivedmesenchymalstemstromalcellsandtheirapplicationinacutekidneyinjury AT kimdohyung reproduciblelargescaleisolationofexosomesfromadiposetissuederivedmesenchymalstemstromalcellsandtheirapplicationinacutekidneyinjury AT chobyongseung reproduciblelargescaleisolationofexosomesfromadiposetissuederivedmesenchymalstemstromalcellsandtheirapplicationinacutekidneyinjury AT yiyongweon reproduciblelargescaleisolationofexosomesfromadiposetissuederivedmesenchymalstemstromalcellsandtheirapplicationinacutekidneyinjury |