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Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury

Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal i...

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Autores principales: Lee, Jun Ho, Ha, Dae Hyun, Go, Hyeon-kyu, Youn, Jinkwon, Kim, Hyun-keun, Jin, Richard C., Miller, Randy B., Kim, Do-hyung, Cho, Byong Seung, Yi, Yong Weon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370182/
https://www.ncbi.nlm.nih.gov/pubmed/32635660
http://dx.doi.org/10.3390/ijms21134774
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author Lee, Jun Ho
Ha, Dae Hyun
Go, Hyeon-kyu
Youn, Jinkwon
Kim, Hyun-keun
Jin, Richard C.
Miller, Randy B.
Kim, Do-hyung
Cho, Byong Seung
Yi, Yong Weon
author_facet Lee, Jun Ho
Ha, Dae Hyun
Go, Hyeon-kyu
Youn, Jinkwon
Kim, Hyun-keun
Jin, Richard C.
Miller, Randy B.
Kim, Do-hyung
Cho, Byong Seung
Yi, Yong Weon
author_sort Lee, Jun Ho
collection PubMed
description Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal injuries in multiple experimental animal models of kidney diseases including AKI. However, the mass production of exosomes is a challenge not only in preclinical studies with large animals but also for successful clinical applications. In this respect, tangential flow filtration (TFF) is suitable for good manufacturing practice (GMP)-compliant large-scale production of high-quality exosomes. Until now, no studies have been reported on the use of TFF, but rather ultracentrifugation has been almost exclusively used, to isolate exosomes for AKI therapeutic application in preclinical studies. Here, we demonstrated the reproducible large-scale production of exosomes derived from adipose tissue-derived MSC (ASC-exosomes) using TFF and the lifesaving effect of the ASC-exosomes in a lethal model of cisplatin-induced rat AKI. Our results suggest the possibility of large-scale stable production of ASC-exosomes without loss of function and their successful application in life-threatening diseases.
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spelling pubmed-73701822020-07-21 Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury Lee, Jun Ho Ha, Dae Hyun Go, Hyeon-kyu Youn, Jinkwon Kim, Hyun-keun Jin, Richard C. Miller, Randy B. Kim, Do-hyung Cho, Byong Seung Yi, Yong Weon Int J Mol Sci Article Acute kidney injury (AKI) is a fatal medical episode caused by sudden kidney damage or failure, leading to the death of patients within a few hours or days. Previous studies demonstrated that exosomes derived from various mesenchymal stem/stromal cells (MSC-exosomes) have positive effects on renal injuries in multiple experimental animal models of kidney diseases including AKI. However, the mass production of exosomes is a challenge not only in preclinical studies with large animals but also for successful clinical applications. In this respect, tangential flow filtration (TFF) is suitable for good manufacturing practice (GMP)-compliant large-scale production of high-quality exosomes. Until now, no studies have been reported on the use of TFF, but rather ultracentrifugation has been almost exclusively used, to isolate exosomes for AKI therapeutic application in preclinical studies. Here, we demonstrated the reproducible large-scale production of exosomes derived from adipose tissue-derived MSC (ASC-exosomes) using TFF and the lifesaving effect of the ASC-exosomes in a lethal model of cisplatin-induced rat AKI. Our results suggest the possibility of large-scale stable production of ASC-exosomes without loss of function and their successful application in life-threatening diseases. MDPI 2020-07-05 /pmc/articles/PMC7370182/ /pubmed/32635660 http://dx.doi.org/10.3390/ijms21134774 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Jun Ho
Ha, Dae Hyun
Go, Hyeon-kyu
Youn, Jinkwon
Kim, Hyun-keun
Jin, Richard C.
Miller, Randy B.
Kim, Do-hyung
Cho, Byong Seung
Yi, Yong Weon
Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury
title Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury
title_full Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury
title_fullStr Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury
title_full_unstemmed Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury
title_short Reproducible Large-Scale Isolation of Exosomes from Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Their Application in Acute Kidney Injury
title_sort reproducible large-scale isolation of exosomes from adipose tissue-derived mesenchymal stem/stromal cells and their application in acute kidney injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370182/
https://www.ncbi.nlm.nih.gov/pubmed/32635660
http://dx.doi.org/10.3390/ijms21134774
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