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Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis

Host genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional vari...

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Autores principales: Borilova Linhartova, Petra, Danek, Zdenek, Deissova, Tereza, Hromcik, Filip, Lipovy, Bretislav, Szaraz, David, Janos, Julius, Fassmann, Antonin, Bartova, Jirina, Drizhal, Ivo, Izakovicova Holla, Lydie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370291/
https://www.ncbi.nlm.nih.gov/pubmed/32630798
http://dx.doi.org/10.3390/ijms21134728
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author Borilova Linhartova, Petra
Danek, Zdenek
Deissova, Tereza
Hromcik, Filip
Lipovy, Bretislav
Szaraz, David
Janos, Julius
Fassmann, Antonin
Bartova, Jirina
Drizhal, Ivo
Izakovicova Holla, Lydie
author_facet Borilova Linhartova, Petra
Danek, Zdenek
Deissova, Tereza
Hromcik, Filip
Lipovy, Bretislav
Szaraz, David
Janos, Julius
Fassmann, Antonin
Bartova, Jirina
Drizhal, Ivo
Izakovicova Holla, Lydie
author_sort Borilova Linhartova, Petra
collection PubMed
description Host genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional variants in selected interleukin (IL) genes in patients with the generalized form of AgP in a homogenous population. Twelve polymorphisms in IL-1 gene cluster, IL-6 and its receptor, IL-10, IL-17A, and IL-18 were determined in 91 AgP patients and 210 controls. Analysis of seven selected periodontal bacteria in subgingival sulci/pockets was performed with a commercial DNA-microarray kit in a subgroup of 76 individuals. The pilot in vitro study included stimulation of peripheral blood monocytes (PBMC) from 20 individuals with periodontal bacteria and measurement of IL-10 levels using the Luminex method. Only the unctional polymorphism IL-10 −1087 A/G (rs1800896) and specific IL-10 haplotypes were associated with the development of the disease (p < 0.05, P(corr) > 0.05). Four bacterial species occurred more frequently in AgP than in controls (p < 0.01, P(corr) < 0.05). Elevated IL-10 levels were found in AgP patients, carriers of IL-10 −1087GG genotype, and PBMCs stimulated by periodontal bacteria (p < 0.05, P(corr) > 0.05). We therefore conclude that a combination of genetic predisposition to the altered expression of IL-10 and the presence of specific periodontal bacteria may contribute to Th1/Th2 balance disruption and AgP development.
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spelling pubmed-73702912020-08-07 Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis Borilova Linhartova, Petra Danek, Zdenek Deissova, Tereza Hromcik, Filip Lipovy, Bretislav Szaraz, David Janos, Julius Fassmann, Antonin Bartova, Jirina Drizhal, Ivo Izakovicova Holla, Lydie Int J Mol Sci Article Host genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional variants in selected interleukin (IL) genes in patients with the generalized form of AgP in a homogenous population. Twelve polymorphisms in IL-1 gene cluster, IL-6 and its receptor, IL-10, IL-17A, and IL-18 were determined in 91 AgP patients and 210 controls. Analysis of seven selected periodontal bacteria in subgingival sulci/pockets was performed with a commercial DNA-microarray kit in a subgroup of 76 individuals. The pilot in vitro study included stimulation of peripheral blood monocytes (PBMC) from 20 individuals with periodontal bacteria and measurement of IL-10 levels using the Luminex method. Only the unctional polymorphism IL-10 −1087 A/G (rs1800896) and specific IL-10 haplotypes were associated with the development of the disease (p < 0.05, P(corr) > 0.05). Four bacterial species occurred more frequently in AgP than in controls (p < 0.01, P(corr) < 0.05). Elevated IL-10 levels were found in AgP patients, carriers of IL-10 −1087GG genotype, and PBMCs stimulated by periodontal bacteria (p < 0.05, P(corr) > 0.05). We therefore conclude that a combination of genetic predisposition to the altered expression of IL-10 and the presence of specific periodontal bacteria may contribute to Th1/Th2 balance disruption and AgP development. MDPI 2020-07-02 /pmc/articles/PMC7370291/ /pubmed/32630798 http://dx.doi.org/10.3390/ijms21134728 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Borilova Linhartova, Petra
Danek, Zdenek
Deissova, Tereza
Hromcik, Filip
Lipovy, Bretislav
Szaraz, David
Janos, Julius
Fassmann, Antonin
Bartova, Jirina
Drizhal, Ivo
Izakovicova Holla, Lydie
Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis
title Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis
title_full Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis
title_fullStr Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis
title_full_unstemmed Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis
title_short Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis
title_sort interleukin gene variability and periodontal bacteria in patients with generalized aggressive form of periodontitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370291/
https://www.ncbi.nlm.nih.gov/pubmed/32630798
http://dx.doi.org/10.3390/ijms21134728
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