Cargando…

Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases

This study aimed to identify trypanosomes infecting cattle in Malawi in order to understand the importance of cattle in the transmission dynamics of Human African Trypanosomiasis (HAT) and Animal African Trypanosomosis (AAT). A total of 446 DNA samples from cattle blood from three regions of Malawi...

Descripción completa

Detalles Bibliográficos
Autores principales: Marsela, Megasari, Hayashida, Kyoko, Nakao, Ryo, Chatanga, Elisha, Gaithuma, Alex Kiarie, Naoko, Kawai, Musaya, Janelisa, Sugimoto, Chihiro, Yamagishi, Junya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370688/
https://www.ncbi.nlm.nih.gov/pubmed/32686644
http://dx.doi.org/10.1051/parasite/2020043
_version_ 1783561020116041728
author Marsela, Megasari
Hayashida, Kyoko
Nakao, Ryo
Chatanga, Elisha
Gaithuma, Alex Kiarie
Naoko, Kawai
Musaya, Janelisa
Sugimoto, Chihiro
Yamagishi, Junya
author_facet Marsela, Megasari
Hayashida, Kyoko
Nakao, Ryo
Chatanga, Elisha
Gaithuma, Alex Kiarie
Naoko, Kawai
Musaya, Janelisa
Sugimoto, Chihiro
Yamagishi, Junya
author_sort Marsela, Megasari
collection PubMed
description This study aimed to identify trypanosomes infecting cattle in Malawi in order to understand the importance of cattle in the transmission dynamics of Human African Trypanosomiasis (HAT) and Animal African Trypanosomosis (AAT). A total of 446 DNA samples from cattle blood from three regions of Malawi were screened for African trypanosomes by ITS1 PCR. The obtained amplicons were sequenced using a portable next-generation sequencer, MinION, for validation. Comparison of the results from ITS1 PCR and MinION sequencing showed that combining the two methods provided more accurate species identification than ITS1 PCR alone. Further PCR screening targeting the serum resistance-associated (SRA) gene was conducted to detect Trypanosoma brucei rhodesiense. Trypanosoma congolense was the most prevalent Trypanosoma sp., which was found in Nkhotakota (10.8%; 20 of 185), followed by Kasungu (2.5%; 5 of 199). Of note, the prevalence of T. b. rhodesiense detected by SRA PCR was high in Kasungu and Nkhotakota showing 9.5% (19 of 199) and 2.7% (5 of 185), respectively. We report the presence of animal African trypanosomes and T. b. rhodesiense from cattle at the human–livestock–wildlife interface for the first time in Malawi. Our results confirmed that animal trypanosomes are important causes of anemia in cattle and that cattle are potential reservoirs for human African trypanosomiasis in Malawi.
format Online
Article
Text
id pubmed-7370688
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher EDP Sciences
record_format MEDLINE/PubMed
spelling pubmed-73706882020-07-21 Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases Marsela, Megasari Hayashida, Kyoko Nakao, Ryo Chatanga, Elisha Gaithuma, Alex Kiarie Naoko, Kawai Musaya, Janelisa Sugimoto, Chihiro Yamagishi, Junya Parasite Research Article This study aimed to identify trypanosomes infecting cattle in Malawi in order to understand the importance of cattle in the transmission dynamics of Human African Trypanosomiasis (HAT) and Animal African Trypanosomosis (AAT). A total of 446 DNA samples from cattle blood from three regions of Malawi were screened for African trypanosomes by ITS1 PCR. The obtained amplicons were sequenced using a portable next-generation sequencer, MinION, for validation. Comparison of the results from ITS1 PCR and MinION sequencing showed that combining the two methods provided more accurate species identification than ITS1 PCR alone. Further PCR screening targeting the serum resistance-associated (SRA) gene was conducted to detect Trypanosoma brucei rhodesiense. Trypanosoma congolense was the most prevalent Trypanosoma sp., which was found in Nkhotakota (10.8%; 20 of 185), followed by Kasungu (2.5%; 5 of 199). Of note, the prevalence of T. b. rhodesiense detected by SRA PCR was high in Kasungu and Nkhotakota showing 9.5% (19 of 199) and 2.7% (5 of 185), respectively. We report the presence of animal African trypanosomes and T. b. rhodesiense from cattle at the human–livestock–wildlife interface for the first time in Malawi. Our results confirmed that animal trypanosomes are important causes of anemia in cattle and that cattle are potential reservoirs for human African trypanosomiasis in Malawi. EDP Sciences 2020-07-20 /pmc/articles/PMC7370688/ /pubmed/32686644 http://dx.doi.org/10.1051/parasite/2020043 Text en © M. Marsela et al., published by EDP Sciences, 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Marsela, Megasari
Hayashida, Kyoko
Nakao, Ryo
Chatanga, Elisha
Gaithuma, Alex Kiarie
Naoko, Kawai
Musaya, Janelisa
Sugimoto, Chihiro
Yamagishi, Junya
Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases
title Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases
title_full Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases
title_fullStr Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases
title_full_unstemmed Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases
title_short Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases
title_sort molecular identification of trypanosomes in cattle in malawi using pcr methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370688/
https://www.ncbi.nlm.nih.gov/pubmed/32686644
http://dx.doi.org/10.1051/parasite/2020043
work_keys_str_mv AT marselamegasari molecularidentificationoftrypanosomesincattleinmalawiusingpcrmethodsandnanoporesequencingepidemiologicalimplicationsforthecontrolofhumanandanimaltrypanosomiases
AT hayashidakyoko molecularidentificationoftrypanosomesincattleinmalawiusingpcrmethodsandnanoporesequencingepidemiologicalimplicationsforthecontrolofhumanandanimaltrypanosomiases
AT nakaoryo molecularidentificationoftrypanosomesincattleinmalawiusingpcrmethodsandnanoporesequencingepidemiologicalimplicationsforthecontrolofhumanandanimaltrypanosomiases
AT chatangaelisha molecularidentificationoftrypanosomesincattleinmalawiusingpcrmethodsandnanoporesequencingepidemiologicalimplicationsforthecontrolofhumanandanimaltrypanosomiases
AT gaithumaalexkiarie molecularidentificationoftrypanosomesincattleinmalawiusingpcrmethodsandnanoporesequencingepidemiologicalimplicationsforthecontrolofhumanandanimaltrypanosomiases
AT naokokawai molecularidentificationoftrypanosomesincattleinmalawiusingpcrmethodsandnanoporesequencingepidemiologicalimplicationsforthecontrolofhumanandanimaltrypanosomiases
AT musayajanelisa molecularidentificationoftrypanosomesincattleinmalawiusingpcrmethodsandnanoporesequencingepidemiologicalimplicationsforthecontrolofhumanandanimaltrypanosomiases
AT sugimotochihiro molecularidentificationoftrypanosomesincattleinmalawiusingpcrmethodsandnanoporesequencingepidemiologicalimplicationsforthecontrolofhumanandanimaltrypanosomiases
AT yamagishijunya molecularidentificationoftrypanosomesincattleinmalawiusingpcrmethodsandnanoporesequencingepidemiologicalimplicationsforthecontrolofhumanandanimaltrypanosomiases