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Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases
This study aimed to identify trypanosomes infecting cattle in Malawi in order to understand the importance of cattle in the transmission dynamics of Human African Trypanosomiasis (HAT) and Animal African Trypanosomosis (AAT). A total of 446 DNA samples from cattle blood from three regions of Malawi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
EDP Sciences
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370688/ https://www.ncbi.nlm.nih.gov/pubmed/32686644 http://dx.doi.org/10.1051/parasite/2020043 |
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author | Marsela, Megasari Hayashida, Kyoko Nakao, Ryo Chatanga, Elisha Gaithuma, Alex Kiarie Naoko, Kawai Musaya, Janelisa Sugimoto, Chihiro Yamagishi, Junya |
author_facet | Marsela, Megasari Hayashida, Kyoko Nakao, Ryo Chatanga, Elisha Gaithuma, Alex Kiarie Naoko, Kawai Musaya, Janelisa Sugimoto, Chihiro Yamagishi, Junya |
author_sort | Marsela, Megasari |
collection | PubMed |
description | This study aimed to identify trypanosomes infecting cattle in Malawi in order to understand the importance of cattle in the transmission dynamics of Human African Trypanosomiasis (HAT) and Animal African Trypanosomosis (AAT). A total of 446 DNA samples from cattle blood from three regions of Malawi were screened for African trypanosomes by ITS1 PCR. The obtained amplicons were sequenced using a portable next-generation sequencer, MinION, for validation. Comparison of the results from ITS1 PCR and MinION sequencing showed that combining the two methods provided more accurate species identification than ITS1 PCR alone. Further PCR screening targeting the serum resistance-associated (SRA) gene was conducted to detect Trypanosoma brucei rhodesiense. Trypanosoma congolense was the most prevalent Trypanosoma sp., which was found in Nkhotakota (10.8%; 20 of 185), followed by Kasungu (2.5%; 5 of 199). Of note, the prevalence of T. b. rhodesiense detected by SRA PCR was high in Kasungu and Nkhotakota showing 9.5% (19 of 199) and 2.7% (5 of 185), respectively. We report the presence of animal African trypanosomes and T. b. rhodesiense from cattle at the human–livestock–wildlife interface for the first time in Malawi. Our results confirmed that animal trypanosomes are important causes of anemia in cattle and that cattle are potential reservoirs for human African trypanosomiasis in Malawi. |
format | Online Article Text |
id | pubmed-7370688 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-73706882020-07-21 Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases Marsela, Megasari Hayashida, Kyoko Nakao, Ryo Chatanga, Elisha Gaithuma, Alex Kiarie Naoko, Kawai Musaya, Janelisa Sugimoto, Chihiro Yamagishi, Junya Parasite Research Article This study aimed to identify trypanosomes infecting cattle in Malawi in order to understand the importance of cattle in the transmission dynamics of Human African Trypanosomiasis (HAT) and Animal African Trypanosomosis (AAT). A total of 446 DNA samples from cattle blood from three regions of Malawi were screened for African trypanosomes by ITS1 PCR. The obtained amplicons were sequenced using a portable next-generation sequencer, MinION, for validation. Comparison of the results from ITS1 PCR and MinION sequencing showed that combining the two methods provided more accurate species identification than ITS1 PCR alone. Further PCR screening targeting the serum resistance-associated (SRA) gene was conducted to detect Trypanosoma brucei rhodesiense. Trypanosoma congolense was the most prevalent Trypanosoma sp., which was found in Nkhotakota (10.8%; 20 of 185), followed by Kasungu (2.5%; 5 of 199). Of note, the prevalence of T. b. rhodesiense detected by SRA PCR was high in Kasungu and Nkhotakota showing 9.5% (19 of 199) and 2.7% (5 of 185), respectively. We report the presence of animal African trypanosomes and T. b. rhodesiense from cattle at the human–livestock–wildlife interface for the first time in Malawi. Our results confirmed that animal trypanosomes are important causes of anemia in cattle and that cattle are potential reservoirs for human African trypanosomiasis in Malawi. EDP Sciences 2020-07-20 /pmc/articles/PMC7370688/ /pubmed/32686644 http://dx.doi.org/10.1051/parasite/2020043 Text en © M. Marsela et al., published by EDP Sciences, 2020 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Marsela, Megasari Hayashida, Kyoko Nakao, Ryo Chatanga, Elisha Gaithuma, Alex Kiarie Naoko, Kawai Musaya, Janelisa Sugimoto, Chihiro Yamagishi, Junya Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases |
title | Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases |
title_full | Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases |
title_fullStr | Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases |
title_full_unstemmed | Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases |
title_short | Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases |
title_sort | molecular identification of trypanosomes in cattle in malawi using pcr methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7370688/ https://www.ncbi.nlm.nih.gov/pubmed/32686644 http://dx.doi.org/10.1051/parasite/2020043 |
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